Recombinant Human Fructose-bisphosphate aldolase B (ALDOB)

In Stock
Code CSB-EP001586HU
Abbreviation Recombinant Human ALDOB protein
MSDS
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Not Test
Target Names
ALDOB
Uniprot No.
Research Area
Cancer
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
2-364aa
Target Protein Sequence
AHRFPALTQEQKKELSEIAQSIVANGKGILAADESVGTMGNRLQRIKVENTEENRRQFREILFSVDSSINQSIGGVILFHETLYQKDSQGKLFRNILKEKGIVVGIKLDQGGAPLAGTNKETTIQGLDGLSERCAQYKKDGVDFGKWRAVLRIADQCPSSLAIQENANALARYASICQQNGLVPIVEPEVIPDGDHDLEHCQYVTEKVLAAVYKALNDHHVYLEGTLLKPNMVTAGHACTKKYTPEQVAMATVTALHRTVPAAVPGICFLSGGMSEEDATLNLNAINLCPLPKPWKLSFSYGRALQASALAAWGGKAANKEATQEAFMKRAMANCQAAKGQYVHTGSSGAASTQSLFTACYTY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Mol. Weight
46.3 kDa
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Gene References into Functions
  1. Silencing Aldolase B activated epithelial markers and repressed mesenchymal markers, indicating inactivation of Aldolase B may lead to inhibition of epithelial-mesenchymal transition PMID: 28558381
  2. The downregulation of ALDOB could indicate a poor prognosis for HCC patients, and therefore, ALDOB might be considered a prognostic biomarker for HCC, especially at the early stage. PMID: 26376879
  3. Single nucleotide polymorphisms in ALDOB, MAP3K1, and MEF2C are associated with cataract. PMID: 25352737
  4. both of exogenous and endogenous ALDOB proteins bind to hepatitis B surface antigen and colocalize in the cytoplasm in vitro and inhibit apoptosis of cisplatin-induced HepG2 cells. PMID: 25072145
  5. Efficient inhibition of aldolase B can prevent high glucose-induced overproduction of methylglyoxal and related cellular dysfunction in endothelial cells. PMID: 22911800
  6. Aldolase B with the A149P substitution has activity that is <100-fold that of the wild type. PMID: 21166391
  7. These novel mutations in ALDOB represent 2% of alleles in American HFI (hereditary fructose intolerance) patients, with IVS1+1G>C representing a significantly higher allele frequency (6%) among HFI patients of Hispanic and African-American ethnicity. PMID: 20882353
  8. This is the first report of six unrelated patients sharing the same ALDOB deletion, thus indicating a founder effect for this allele. PMID: 20848650
  9. Biochemical study of defective aldolase B enzymes is key to revealing the molecular basis of the disease and providing a stronger basis for improved treatment and diagnosis PMID: 20162364
  10. Structural and functional analysis of aldolase B mutants related to hereditary fructose intolerance. PMID: 12417303
  11. The enzyme's structure and function is investigated as a function of temperature.The implications of these structural alterations are discussed with regard to the HFI disease PMID: 12464284
  12. expression of three (Beclin 1, RbAp48 and Pir51) were increased and one (aldolase b) was decreased in liver tumor tissues PMID: 14966907
  13. six new aldolase B mutations in seven unrelated hereditary fructose intolerant (HFI) Italian patients PMID: 15532022
  14. Based on these data and after correction for less common and private ALDOB mutations, hereditary fructose intolerance (HFI) prevalence in central Europe is estimated to be 1:26,100 (95% confidence interval 1: 12,600-79,000). PMID: 15880727
  15. Reverse-hybridization assay tested for an accurate and robust screening tool to identify common ALDOB mutations. PMID: 17292585
  16. Usefulness of ALDOB mutation in screening for diagnosis of hereditary fructose intolerance. PMID: 17457694
  17. there is an important role for physical association between aldolase and the A, B and E subunits of V-ATPase in the regulation of the proton pump PMID: 17576770
  18. Hereditary fructose intolerance with the mutation c.479_482 del AACA PMID: 17955389
  19. Sixteen different mutations of the aldolase B (ALDOB) gene were identified in hereditary fructose intolerance patients. PMID: 18541450
  20. The five gene transcripts (aldolase B, elafin, MST-1, simNIPhom and SLC6A14) were changed in patients with ulcerative colitis, and were related to the disease activity. PMID: 18700007

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Involvement in disease
Hereditary fructose intolerance (HFI)
Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite.
Protein Families
Class I fructose-bisphosphate aldolase family
Database Links

HGNC: 417

OMIM: 229600

KEGG: hsa:229

STRING: 9606.ENSP00000363988

UniGene: Hs.530274

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