Recombinant Human G patch domain-containing protein 3 (GPATCH3)

Code CSB-YP822227HU
MSDS
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Source Yeast
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Code CSB-EP822227HU
MSDS
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Source E.coli
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Code CSB-EP822227HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP822227HU
MSDS
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Source Baculovirus
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Code CSB-MP822227HU
MSDS
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Source Mammalian cell
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Product Details

Purity
≥85% (SDS-PAGE)
Target Names
GPATCH3
Uniprot No.
Alternative Names
DKFZp686E0221; FLJ12455; G patch domain containing 3; G patch domain containing protein 3; G patch domain-containing protein 3; GPATC3; GPATCH3; GPTC3_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-525
Target Protein Sequence
MAVPGEAEEE ATVYLVVSGI PSVLRSAHLR SYFSQFREER GGGFLCFHYR HRPERAPPQA APNSALIPTD PAAEGQLLSQ TSATDVRPLS TRDSTPIQTR TCCCVISVRG LAQAQRLIRM YSGRRWLDSH GTWLPGRCLI RRLRLPTEAS GLGSFPFKTR KELQSWKAEN EAFTLADLKQ LPELNPPVLM PRGNVGTPLR VFLELIRACR LPPRIITQLQ LQFPKTGSSR RYGNVPFEYE DSETVEQEEL VYTAEGEEIP QGTYLADIPA SPCGEPEEEV GKEEEEESHS DEDDDRGEEW ERHEALHEDV TGQERTTEQL FEEEIELKWE KGGSGLVFYT DAQFWQEEEG DFDEQTADDW DVDMSVYYDR DGGDKDARDS VQMRLEQRLR DGQEDGSVIE RQVGTFERHT KGIGRKVMER QGWAEGQGLG CRCSGVPEAL DSDGQHPRCK RGLGYHGEKL QPFGQLKRPR RNGLGLISTI YDEPLPQDQT ESLLRRQPPT SMKFRTDMAF VRGSSCASDS PSLPD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in transcriptional regulation. It is able to activate transcription from the CXCR4 promoter and therefore it might control neural crest cell migration involved in ocular and craniofacial development. Is a negative regulator of immune antiviral response, acting via down-regulation of RIG-I-like receptors signaling and inhibition of type I interferon production. The control mechanism involves interaction with mitochondrial MAVS and inhibition of MAVS assembly with downstream proteins implicated in antiviral response, such as TBK1 and TRAF6.
Gene References into Functions
  1. The results show the existence of high genetic heterogeneity in congenital glaucoma and the role of GPATCH3 in this disease. GPATCH3 is a new gene involved in ocular and craniofacial development. PMID: 28397860
Involvement in disease
GPATCH3 variants may be involved in the pathogenesis of congenital glaucoma, an ocular disease characterized by marked increase of intraocular pressure at birth or early childhood, large ocular globes (buphthalmos) and corneal edema. It results from developmental defects of the trabecular meshwork and anterior chamber angle of the eye that prevent adequate drainage of aqueous humor.
Subcellular Location
Nucleus. Cytoplasm.
Tissue Specificity
Expressed in ocular tissues including retinal pigment epithelium, cornea, ciliary muscle and non-pigmented ciliary epithelium. Also expressed in optic nerve, cartilage, skin and lymph node.
Database Links

HGNC: 25720

OMIM: 617486

KEGG: hsa:63906

UniGene: Hs.10903

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