Recombinant Human GRB2-related adapter protein 2 (GRAP2)

Code CSB-YP009884HU
MSDS
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Source Yeast
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Code CSB-EP009884HU
MSDS
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Source E.coli
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Code CSB-EP009884HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009884HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP009884HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Adapter protein GRID; GADS; GADS protein; GRAP-2; Grap2; GRAP2_HUMAN; GRB-2-like protein; GRB2-related adapter protein 2; GRB2-related adaptor protein 2; GRB2-related protein with insert domain ; GRB2L; GRBLG; GRBX; Grf-40; Grf40 adapter protein; Grf40; GRID; Growth factor receptor-binding protein; Growth factor receptor-bound protein 2-related adaptor protein 2 ; GRPL; Hema; Hematopoietic cell-associated adapter protein GrpL; Hematopoietic cell-associated adaptor protein GRPL ; Mona; p38; Protein GADS; SH3-SH2-SH3 adapter Mona; SH3-SH2-SH3 adaptor molecule
Species
Homo sapiens (Human)
Expression Region
1-330
Target Protein Sequence
MEAVAKFDFT ASGEDELSFH TGDVLKILSN QEEWFKAELG SQEGYVPKNF IDIQFPKWFH EGLSRHQAEN LLMGKEVGFF IIRASQSSPG DFSISVRHED DVQHFKVMRD NKGNYFLWTE KFPSLNKLVD YYRTNSISRQ KQIFLRDRTR EDQGHRGNSL DRRSQGGPHL SGAVGEEIRP SMNRKLSDHP PTLPLQQHQH QPQPPQYAPA PQQLQQPPQQ RYLQHHHFHQ ERRGGSLDIN DGHCGTGLGS EMNAALMHRR HTDPVQLQAA GRVRWARALY DFEALEDDEL GFHSGEVVEV LDSSNPSWWT GRLHNKLGLF PANYVAPMTR
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc.
Gene References into Functions
  1. These data are consistent with a model in which bivalent recruitment of a GADS/SLP-76 complex is required for costimulation by CD6. PMID: 28289074
  2. Following phosphorylation of the tyrosine, the proteins growth factor receptor-bound protein 2 (Grb2), Grb2-related adaptor downstream of Shc (Gads), and p85 subunit of phosphoinositide 3-kinase may bind to pYMNM (where pY is phosphotyrosine) via their Src homology 2 (SH2) domains, leading to downstream signaling to distinct immune pathways. These three adaptor proteins bind to the same site on CD28 with variable affinity PMID: 27927989
  3. GADS deficient T cells displayed similar levels of T cell receptor -induced SLP-76 and PLC-gamma1 phosphorylation but exhibited substantial decrease in TCR-induced IL-2 and IFN-gamma release. PMID: 25636200
  4. Gads was dispensable for TCR-induced phosphorylation of SLP-76, but was a dose-dependent amplifier of TCR-induced CD69 expression. PMID: 25452106
  5. GADS mediates lymphoid disease downstream of BCR-ABL through the recruitment of specific signaling intermediates. PMID: 23399893
  6. Histidine domain-protein tyrosine phosphatase interacts with Grb2 and GrpL PMID: 21179510
  7. The results show that Bcr-Abl regulates the actin cytoskeleton and non-apoptotic membrane blebbing via a GADS/Slp-76/Nck1 adaptor protein pathway. PMID: 20079431
  8. Upstream open reading frames regulate translation of this protein's mRNA in megakaryocytes. PMID: 12487779
  9. Exogenous expression of GrpL in a GrpL-negative B cell line leads to enhanced antigen receptor-induced extracellular signal-related kinase and p38 mitogen-activated protein kinase phosphorylation. PMID: 12496419
  10. AML-1 plays a role in driving Mona protein expression in T and myelomonocytic cells. PMID: 12554803
  11. Plays a tissue-specific role as an inhibitor of RET receptor tyrosine kinase mitogenic signaling PMID: 12917638
  12. Gads plays a dominant role in CD28-mediated IL-2 promoter activation. PMID: 16818765
  13. The integrity of T-cell receptor signaling in vivo is sustained both by strong selection of SLP-76 for the Gads C-SH3 domain and by a capacity to buffer intrinsic crossreactivity. PMID: 17235283
  14. Consistent Grap-2 expression suggests a specific role for this adaptor in human medullary thyroid carcinoma, while qualitative alterations do not appear to influence RET signaling PMID: 19027225

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Subcellular Location
Nucleus. Cytoplasm. Endosome.
Protein Families
GRB2/sem-5/DRK family
Database Links

HGNC: 4563

OMIM: 604518

KEGG: hsa:9402

STRING: 9606.ENSP00000339186

UniGene: Hs.517499

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