Recombinant Human GTP-binding protein 10 (GTPBP10)

Code CSB-YP010032HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP010032HU
MSDS
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Source E.coli
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Code CSB-EP010032HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP010032HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP010032HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GTPBP10
Uniprot No.
Alternative Names
GTPBP10; OBGH2; UG0751c10GTP-binding protein 10; Protein obg homolog 2; ObgH2
Species
Homo sapiens (Human)
Expression Region
1-387
Target Protein Sequence
MVHCSCVLFR KYGNFIDKLR LFTRGGSGGM GYPRLGGEGG KGGDVWVVAQ NRMTLKQLKD RYPRKRFVAG VGANSKISAL KGSKGKDCEI PVPVGISVTD ENGKIIGELN KENDRILVAQ GGLGGKLLTN FLPLKGQKRI IHLDLKLIAD VGLVGFPNAG KSSLLSCVSH AKPAIADYAF TTLKPELGKI MYSDFKQISV ADLPGLIEGA HMNKGMGHKF LKHIERTRQL LFVVDISGFQ LSSHTQYRTA FETIILLTKE LELYKEELQT KPALLAVNKM DLPDAQDKFH ELMSQLQNPK DFLHLFEKNM IPERTVEFQH IIPISAVTGE GIEELKNCIR KSLDEQANQE NDALHKKQLL NLWISDTMSS TEPPSKHAVT TSKMDII
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May be involved in the ribosome maturation process. Complements an ObgE(CgtA) function in E.coli ribosome maturation. Plays a role of GTPase in vitro. When missing, disorganization of the nucleolar architecture is observed.
Gene References into Functions
  1. Knock-down of ObgH1 by RNAi induced mitochondria elongation, whereas knock-down of ObgH2 resulted in the disorganization of the nucleolar architecture. PMID: 17054726
Subcellular Location
Nucleus, nucleolus. Chromosome. Note=Found in the dense fibrillar compartment region of the nucleolus. At the onset of mitosis moves to the chromosome surface and remains there until anaphase. Gradually re-assembles into the nucleolus at late anaphase to telophase.
Protein Families
TRAFAC class OBG-HflX-like GTPase superfamily, OBG GTPase family
Database Links

HGNC: 25106

OMIM: 610920

KEGG: hsa:85865

STRING: 9606.ENSP00000222511

UniGene: Hs.593547

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