Product Details

Purity

>85% (SDS-PAGE)

Target Names

LIPF

Uniprot No.

P07098

Alternative Names

Gastric lipase; Gastric triacylglycerol lipase; gL; HGL; HLAL; LIPF; LIPG_HUMAN

Species

Homo sapiens (Human)

Expression Region

20-398

Target Protein Sequence

L FGKLHPGSPE VTMNISQMIT YWGYPNEEYE VVTEDGYILE VNRIPYGKKN SGNTGQRPVV FLQHGLLASA TNWISNLPNN SLAFILADAG YDVWLGNSRG NTWARRNLYY SPDSVEFWAF SFDEMAKYDL PATIDFIVKK TGQKQLHYVG HSQGTTIGFI AFSTNPSLAK RIKTFYALAP VATVKYTKSL INKLRFVPQS LFKFIFGDKI FYPHNFFDQF LATEVCSREM LNLLCSNALF IICGFDSKNF NTSRLDVYLS HNPAGTSVQN MFHWTQAVKS GKFQAYDWGS PVQNRMHYDQ SQPPYYNVTA MNVPIAVWNG GKDLLADPQD VGLLLPKLPN LIYHKEIPFY NHLDFIWAMD APQEVYNDIV SMISEDKK

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

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■ Q&A

Please can you provide sequence, pricing, availability and tag information for all available sizes?
Have you received any customer feedback regarding activity of this protein?

Thanks for your inquiry.
Expression Region: 20-398aa; Full length of the mature protein.
Tag information:Tag type will be determined during the manufacturing process.
The expected tag is N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:
You can view the full sequence and tag information on this page.
Regarding the activity, sorry, we haven't received the feedback from other customers, and we also haven't detected the activity before, it's not 100% guaranteed.
However, as what we provide is the full length of the mature protein and we use the affinity chromatography method to purify in a mild mild condition, theoretically, it should be active. We can share a literature for your reference.

Target Background

Function

Catalyzes the hydrolysis of triacylglycerols to yield free fatty acids, diacylglycerol, monoacylglycerol, and glycerol. Shows a preferential hydrolysis at the sn-3 position of triacylglycerol.

Gene References into Functions

site-directed mutagenesis the role of three key residues (K4, E225, R229) involved in salt bridges stabilizing the lid domain that controls the access to the active site and is part of the interfacial recognition site. Their substitution has an impact on the pH-dependent activity of rHGL and its relative activities on medium and long chain triglycerides. PMID: 28694218
LIPFDGKA might serve as a potential possible biomarkers for diagnosis of gastric cancer, and their downregulation may bring new perspective into the investigation of gastric cancer prognosis PMID: 27498782
In patients with intrahepatic cholestasis of pregnancy, there was no elevation in LIPF mRNA in maternal circulation compared with controls. PMID: 25059952
Recombinant human gastric lipase (hGL) was transiently expressed in Nicotiana benthamiana leaves using the CPMV-HT expression system. PMID: 21945702
insights into the domain movements PMID: 20965171
down-regulated KLF4, CHGA, GPX3, SST and LIPF, together with up-regulated SERPINH1, THY1 and INHBA is an 8-gene signature for gastric cancer PMID: 20043075
Mechanisms of inhibition of triacylglycerol hydrolysis PMID: 11940604
identidfied the cap and the lid residues involved in the binding with the lipidic substrate PMID: 12941646

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Subcellular Location

Secreted.

Protein Families

AB hydrolase superfamily, Lipase family

Database Links

HGNC: 6622

OMIM: 601980

KEGG: hsa:8513

STRING: 9606.ENSP00000377900

UniGene: Hs.523130

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Code	CSB-BP012976HU
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Code	CSB-EP012976HU-B
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Recombinant Human Gastric triacylglycerol lipase (LIPF)

Product Details

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Target Background

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