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Recombinant Human Glucosamine-6-phosphate isomerase 1 (GNPDA1)

Recombinant Human Glucosamine-6-phosphate isomerase 1 (GNPDA1)

Code	CSB-EP009630HU
Abbreviation	Recombinant Human GNPDA1 protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

GNPDA1

Uniprot No.

P46926

Research Area

Metabolism

Alternative Names

EC 3.5.99.6; GlcN6P deaminase 1; Glucosamine 6 phosphate deaminase 1; Glucosamine 6 phosphate isomerase 1; Glucosamine-6-phosphate deaminase 1; Glucosamine-6-phosphate isomerase 1; GNPDA 1; GNPDA1; GNPI; GNPI1_HUMAN; HLN; KIAA0060; Oscillin; Putative glucosamine 6 phosphate isomerase

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-289aa

Target Protein Sequence

MKLIILEHYSQASEWAAKYIRNRIIQFNPGPEKYFTLGLPTGSTPLGCYKKLIEYYKNGDLSFKYVKTFNMDEYVGLPRDHPESYHSFMWNNFFKHIDIHPENTHILDGNAVDLQAECDAFEEKIKAAGGIELFVGGIGPDGHIAFNEPGSSLVSRTRVKTLAMDTILANARFFDGELTKVPTMALTVGVGTVMDAREVMILITGAHKAFALYKAIEEGVNHMWTVSAFQQHPRTVFVCDEDATLELKVKTVKYFKGLMLVHNKLVDPLYSIKEKETEKSQSSKKPYSD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

36.7kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This Human GNPDA1 recombinant protein was produced in E.coli, where the gene sequence encoding Human GNPDA1 (1-289aa) was expressed with the N-terminal 6xHis tag. The purity of this GNPDA1 protein was greater than 90% by SDS-PAGE.
Glucosamine-6-phosphate isomerase 1 (GNPDA1) is an enzyme that is involved in energy metabolism. It has been studied in the context of hepatocellular carcinoma (HCC), a type of liver cancer. High expression of GNPDA1 has been found to be associated with advanced tumor stage, TNM stage, and grade in HCC patients. In HCC cells, GNPDA1 promotes proliferation, migration, and invasion, while inhibiting apoptosis. This suggests that GNPDA1 may serve as a prognostic biomarker and potential therapeutic target for HCC.

Reference:
Li D, Cheng X, Zheng W, Chen J. Glucosamine-6-Phosphate Isomerase 1 Promotes Tumor Progression and Indicates Poor Prognosis in Hepatocellular Carcinoma. Cancer Manag Res. 2020 Jun 24;12:4923-4935.

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Target Background

Function

Seems to trigger calcium oscillations in mammalian eggs. These oscillations serve as the essential trigger for egg activation and early development of the embryo.

Gene References into Functions

Allosteric kinetics of the isoform 1 of human glucosamine-6-phosphate deaminase. PMID: 21807125
identification, chromosomal localization and functional analysis of cDNA encoding GNPI/Oscillin homolog named GNPI2. PMID: 12616532
Sequence analysis and crystallographic structure of GNP1. PMID: 12965206

Subcellular Location

Cytoplasm.

Protein Families

Glucosamine/galactosamine-6-phosphate isomerase family

Database Links

HGNC: 4417

OMIM: 601798

KEGG: hsa:10007

STRING: 9606.ENSP00000311876

UniGene: Hs.633853

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