Recombinant Human Glucose-6-phosphate 1-dehydrogenase(G6PD)

Code CSB-YP009121HU
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Source Yeast
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Code CSB-EP009121HU
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Source E.coli
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Code CSB-EP009121HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009121HU
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Source Baculovirus
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Code CSB-MP009121HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names G6PD
Uniprot No. P11413
Alternative Names G6PD; G6PD_HUMAN; G6PD1; G6pdx; Glucose 6 phosphate 1 dehydrogenase; Glucose 6 phosphate dehydrogenase; Glucose 6 phosphate dehydrogenase, G6PD; Glucose-6-phosphate 1-dehydrogenase; MET19; POS10; Zwf1p
Species Homo sapiens (Human)
Expression Region 2-515
Target Protein Sequence AEQVALSRT QVCGILREEL FQGDAFHQSD THIFIIMGAS GDLAKKKIYP TIWWLFRDGL LPENTFIVGY ARSRLTVADI RKQSEPFFKA TPEEKLKLED FFARNSYVAG QYDDAASYQR LNSHMNALHL GSQANRLFYL ALPPTVYEAV TKNIHESCMS QIGWNRIIVE KPFGRDLQSS DRLSNHISSL FREDQIYRID HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP FGTEGRGGYF DEFGIIRDVM QNHLLQMLCL VAMEKPASTN SDDVRDEKVK VLKCISEVQA NNVVLGQYVG NPDGEGEATK GYLDDPTVPR GSTTATFAAV VLYVENERWD GVPFILRCGK ALNERKAEVR LQFHDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPG MFFNPEESEL DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH QIELEKPKPI PYIYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
Gene References into Functions
  1. Study identified G6PD as a downstream target of miR1 in pituitary tumor cells and suggested that G6PD plays important roles for mediating the inhibitory effect of miR1 on the growth of pituitary tumor cells. PMID: 30272333
  2. Study demonstrated that high G6PD expression is a poor prognostic factor in bladder cancer, and the levels of G6PD expression increase with increasing tumor stage. Patients with bladder cancer with high G6PD expression had worse survival rates compared with those with lower G6PD expression in resected tumors. PMID: 30066842
  3. G6PD-dificient patients were found to have an increased risk for cancers, especially for brain tumor; higher tumor G6PD expression was associated with poor patient survival in low grade glioma (LGG), but not in Glioblastoma multiforme. A prognostication model using expression levels of G6PD and 9 related genes (PSMA2, PSMB8, SHFM1, GSS, GSTK1, MGST2, POLD3, MSH2, MSH6) could independently predict LGG patient survival. PMID: 29845423
  4. G6PD contributes to HCC migration and invasion of hepatocellular carcinoma cells by inducing epithelial-mesenchymal transition through activation of signal transducer and activator of transcription 3 PMID: 29471502
  5. Study provided detailed genotypes of G6PD deficiency in Guangdong province with minorities populations and identified various point mutations in G6PD gene. PMID: 30077011
  6. Results showed that not only G6PD expression but also G6PD activity was significantly lowered along with 3D MCF-7 cells culture time. PMID: 29291545
  7. An aggregate analysis of mosaic G6PD expression in two distinct ethnic cohorts has been reported. PMID: 29240263
  8. Proportion of mutational types in G6PD and the degree of enzyme activity change in various mutational types were found in the neonates of Fujian Province. Three most common mutation types were c.1376G > T, c.1388G > A, and c.95A >G. PMID: 30045279
  9. Chemical inhibitors against SIRT2 suppress G6PD activity, leading to reduced cell proliferation of leukaemia cells, but not normal hematopoietic stem and progenitor cells. Importantly, SIRT2 is overexpressed in clinical acute myeloid leukaemia samples, while K403 acetylation is downregulated and G6PD catalytic activity is increased comparing to that of normal control. PMID: 27586085
  10. Treatment of erythrocytes with Bay 11-7082, parthenolide or DMF led to concentration-dependent eryptosis resulting from complete depletion of GSH. GSH depletion was due to strong inhibition of G6PDH activity. PMID: 27353740
  11. Studies indicate that the activities of glucose-6-phosphate dehydrogenase (the rate-limiting enzyme in the pentose shunt) and glucose flux through the shunt pathway is increased in various lung cells including, the stem cells, in pulmonary hypertension. PMID: 29047080
  12. These findings revealed a novel glucose metabolism-related mechanism of PAK4 in promoting colon cancer cell growth, suggesting that PAK4 and/or G6PD blockage might be a potential therapeutic strategy for colon cancer. PMID: 28542136
  13. Aggregated across all genotypes, we find that increasing levels of G6PD deficiency are associated with decreasing risk of cerebral malaria, but with increased risk of severe malarial anaemia. PMID: 28067620
  14. We concluded that the MeltPro G6PD assay is useful as a diagnostic or screening tool for G6PD deficiency in clinical settings PMID: 27495838
  15. The glucose-6-phosphate dehydrogenase enzymatic deficiency was significantly higher in males compared to females in Burkina Faso. (Review) PMID: 29169341
  16. This work expands the current understanding of the biochemical underpinnings of G6PD variant pathogenicity. PMID: 28297664
  17. This study assessed quantitatively the hemolytic risk with tafenoquine in female healthy volunteers heterozygous for the Mahidol(487A) glucose-6-phosphate dehydrogenase (G6PD)-deficient variant versus G6PD-normal females. PMID: 28749773
  18. only in the case of G6PD and TALDO, the ratio of BrdU incorporation to DNA was significantly changed. The presented results together with our previously published studies illustrate the complexity of the influence of genes coding for central carbon metabolism on the control of DNA replication in human fibroblasts, and indicate which of them are especially important in this process. PMID: 28887160
  19. Data suggest that G6PD PT materials can be stored at 4 degrees C and used for up to one month and can be stored at -20 degrees C for one year and yield >90% enzyme activity. Exposure to warm temperatures, especially with elevated humidity, should be avoided. Desiccant should always be used to mitigate humidity effects PMID: 28479150
  20. We found that 2 G6PD variant genotypes were associated with elevated sTfR concentrations, which limits the accuracy of sTfR as a biomarker of iron status in this population. PMID: 28768839
  21. immature reticulocytes (CD71+) targeted by P. vivax invasion are enzymatically normal, even in hemizygous G6PD-Mahidol G487A mutants; thus, allowing the normal growth, development, and high parasite density in severely deficient samples PMID: 28591790
  22. review of the state of the art in G6PD deficiency, describing 217 mutations in the g6pd gene; also compiled information about 31 new mutations, 16 that were not recognized and 15 more that have recently been reported; found that class I mutations have the most deleterious effects on the structure and stability of the protein PMID: 27941691
  23. We now provide an additional evidence form Palestinian G6PD-deficient subjects for a possible role of 3' UTR c.*+357 A>G, c.1365-13T>C, and/or c.1311C>T polymorphism for G6PD deficiency, suggesting that not only a single variation in the exonic or exonic intronic boundaries, but also a haplotype of G6PD should considered as a cause for G6PD deficiency. PMID: 28059001
  24. This work reviews and discusses rationales and challenges of G6PD screening program in Gaza Strip. We advocate adopting a national neonatal G6PD screening program in Gaza Strip to identify children at risk and promote wellness and health for Palestine. PMID: 27064064
  25. From the study it appears that Ala44Gly and Gly163Ser are the most common G6PD mutations in Dhaka, Bangladesh. This is the first study of G6PD mutations in Bangladesh. PMID: 27880809
  26. the anti-cervix cancer mechanism of G6PD deficiency may be involved with the decreased cancer cells migration and proliferation ability as a result of abnormal reorganization of cell cytoskeleton and abnormal biomechanical properties caused by the increased reactive oxygen species. PMID: 27217331
  27. A novel pathogenic missense mutation in G6PD is associated with BCGitis in two patients with G6PD deficiency. PMID: 27914961
  28. Results show that G6PD mutations in the Mexican population affect the catalytic properties and structural parameters regardless of the distance from the active site changing the three-dimensional structure which correlates with a more severe clinically phenotype. Also, the global stability of the protein is affected. PMID: 27213370
  29. G6PD may function as an important regulator in development and progression of esophageal squamous cell carcinoma by manipulating STAT3 signaling pathway PMID: 26250461
  30. One novel mutation (p.Cys385Gly, labeled G6PD Kangnam) and two known mutations [p.Ile220Met (G6PD Sao Paulo) and p.Glu416Lys (G6PD Tokyo)] were identified. glucose-6-phosphate dehydrogenase (G6PD) PMID: 28028996
  31. Increased advanced lipoxidation end products generation was associated with decreased G6PD activity in mild non-proliferative diabetic retinopathy. PMID: 27916496
  32. Increased G6PD expression is associated with triple-negative breast cancer. PMID: 26715276
  33. Glucose-6-phosphate dehydrogenase variants are associated with glucose-6-phosphate dehydrogenase deficiency. PMID: 26827633
  34. Common G6PD variant identification using Amplification resistant modified system (ARMS)-PCR in a Tunisian population. PMID: 27029726
  35. erythrocyte G6PD and their correlation with GSH provides evidence of a higher oxidative stress in old age population PMID: 26711700
  36. Data indicate a tumor suppressor-like function of Bcl-2 associated athanogene 3 (BAG3) via direct interaction with glucose 6 phosphate dehydrogenase (G6PD) in hepatocellular carcinomas (HCCs) at the cellular level. PMID: 26621836
  37. This is the first report of G6PD deficiency among Chinese Hakka population in Jiangxi province. PMID: 26823837
  38. These findings suggest that the increased susceptibility of the G6PD-knockdown cells to viral infection was due to impaired NF-kappaB signaling and antiviral response mediated by HSCARG. PMID: 26694452
  39. differential expression in children with allergic asthma PMID: 25979194
  40. G6PD deficiency affects the cellular inflammatory response and the decreased TNF-alpha-mediated antiviral response in G6PD-kd A549 cells is a result of dysregulated NOX/MAPK/NF-kappaB/COX-2 signaling PMID: 27097228
  41. study demonstrated that G6PD molecular deficiency was not associated either with clinical ischemic stroke PMID: 26840990
  42. Transgenic mice moderately overexpressing G6PD have higher levels of NADPH, lower levels of ROS-derived damage, and better protection from ageing-associated functional decline, including extended median lifespan in females. PMID: 26976705
  43. Overexpression of G6PD is associated with high risks of recurrent metastasis in primary breast carcinoma. PMID: 26607846
  44. G6PD glycosylation is increased in human lung cancers. Glycosylation activates G6PD activity and increases glucose flux through the PPP, thereby providing precursors for nucleotide and lipid biosynthesis, and reducing equivalents for antioxidant defense. PMID: 26399441
  45. To understand the sporadic Alzheimer's disease, the writer of this paper thinks that, looking into a crystal ball might not yield much of a benefit but glucose-6-phosphate dehydrogenase deficiency could effortlessly give some clues. PMID: 26004559
  46. Mutation of G6PD is associated with G6PD deficiency in Chinese population. PMID: 26829728
  47. G6PD deficiency is an example of balanced polymorphism, in which high rate of mortality caused by this disorder is offset by the protection that it offers against Plasmodium falciparum malaria PMID: 26139767
  48. G6PD, GGCT, IDH1, isocitrate dehydrogenase 2 (NADP+,mitochondrial) (IDH2) and glutathione S-transferase pi 1(GSTP1), five of the critical components of GSH pathway, contribute to chemoresistance. PMID: 25818003
  49. Proteomics results showed that G6PD was highly expressed in cervical cancer cells and its down regulation reduced reduced the capacity of HeLa cells to migrate and invade in vitro. PMID: 25633909
  50. Studied G6PD mutations present in a Sri Lankan population and their association with uncomplicated malaria. PMID: 25885177

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Involvement in disease Anemia, non-spherocytic hemolytic, due to G6PD deficiency (NSHA)
Subcellular Location Cytoplasm, cytosol. Membrane; Peripheral membrane protein.
Protein Families Glucose-6-phosphate dehydrogenase family
Tissue Specificity Isoform Long is found in lymphoblasts, granulocytes and sperm.
Database Links

HGNC: 4057

OMIM: 300908

KEGG: hsa:2539

STRING: 9606.ENSP00000377192

UniGene: Hs.461047

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