Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

HBD

Uniprot No.

P02042

Research Area

Transport

Alternative Names

Delta-globin Hemoglobin delta chain

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

2-147aa

Target Protein Sequence

VHLTPEEKTAVNALWGKVNVDAVGGEALGRLLVVYPWTQRFFESFGDLSSPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFSQLSELHCDKLHVDPENFRLLGNVLVCVLARNFGKEFTPQMQAAYQKVVAGVANALAHKYH
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

47.5 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 2-147 constitute the expression domain of recombinant Human HBD. The calculated molecular weight for this HBD protein is 47.5 kDa. This HBD recombinant protein is manufactured in e.coli. The HBD gene fragment has been modified by fusing the N-terminal 6xHis-GST tag, providing convenience in detecting and purifying the recombinant HBD protein during the following stages.

The human hemoglobin subunit delta (HBD) is a protein that is part of the hemoglobin molecule, responsible for oxygen transport in red blood cells. HBD is located on chromosome 11. Hemoglobin is composed of four subunits – two alpha globin chains and two beta globin chains. The HBD expression is primarily restricted to the embryonic and fetal stages of development. In adults, the delta globin chain is replaced by the beta-globin chain. Hemoglobin containing the delta subunit is known as fetal hemoglobin (HbF). Understanding the regulation of hemoglobin subunit expression is crucial for comprehending normal hemoglobin function and disorders like thalassemias and sickle cell disease.

Target Background

Function

Involved in oxygen transport from the lung to the various peripheral tissues.

Gene References into Functions

Blood donor homozygous for Hb D Los Angeles. PMID: 25217459
The expression of the activated delta-globin gene in a beta-thalassemia mice model greatly improves the phenotype, validating the delta-globin chain as a therapeutic target for beta-hemoglobinopathies. PMID: 23872310
Data indicate that a delta-globin mutation was suspected in both patients. PMID: 23806011
Twenty-one different mutations were detected, and of these 12 have not been previously described. PMID: 23215833
A functional promoter polymorphism of the delta-globin gene is a specific marker of the Arab-Indian haplotype PMID: 22641479
This report describes a novel missense mutation in delta-globin (HBD: c.323G>A, Gly > Asp) in an Indian family with heterozygous beta-thalassemia with normal HbA(2) levels. PMID: 22477537
The 5' breakapoint of the (deltabeta)(0) thalassemia deletion in a compound heterozygote was located in the second intron of the delta globin gene. PMID: 11860449
Hb A2-Monreale [delta146(HC3)His-->Arg]is a novel delta chain variant. PMID: 11939506
alternate mRNA species in adult erythroid cells; mRNA encodes an additional 145 nt in the upstream untranslated region, suggesting an alternative site of transcriptional initiation and transcription through the previously defined promoter PMID: 15234005
The atomic coordinates of the delta-chain of hemoglobin A2 (R2 state) are used to model the structure of hemoglobin homotetramer delta 4, which occurs in rare hemoglobin H disease. PMID: 15449937
Deletion pf this geneis a common, and possibly the predominant beta-thalassemia mutation of the Austroasiatic Lao Theung population. PMID: 15757827
characterization of the delta-globin gene alleles and of RFLP-haplotypes, SNPs and one microsatellite associated with them in 231 carriers originating principally from East Sicily; Seventeen alleles were identified, of which five were new PMID: 18221842
Borderline HbA(2) is not a rare event in a population with a high prevalence of beta-thalassemia carriers PMID: 18603555
Unusually low levels of HBA2 in a compound heterozygote can be explained from the functional inhibition of the HBD gene, in cis to the HBG1 gene, bearing the nd-HPFH mutation. PMID: 18615450
Hb A2 IVS-I-5 (G > C) beta-thalassemia mutation phenotype in 289 carriers was reported. PMID: 18932068

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Protein Families

Globin family

Tissue Specificity

Red blood cells.

Database Links

HGNC: 4829

OMIM: 142000

KEGG: hsa:3045

STRING: 9606.ENSP00000369654

UniGene: Hs.699280

Code	CSB-EP010152HU
Abbreviation	Recombinant Human HBD protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Hemoglobin subunit delta (HBD)

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