Recombinant Human High mobility group protein 20A (HMG20A)

Code CSB-YP865090HU
MSDS
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Source Yeast
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Code CSB-EP865090HU
MSDS
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Source E.coli
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Code CSB-EP865090HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP865090HU
MSDS
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Source Baculovirus
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Code CSB-MP865090HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HMG20A
Uniprot No.
Alternative Names
FLJ10739; High mobility group 20A; High mobility group protein 20A; HM20A_HUMAN; HMG box containing protein 20A; HMG box domain containing 1; HMG box-containing protein 20A; HMG domain protein HMGX1 ; HMG domain-containing protein 1; HMG domain-containing protein HMGX1; hmg20a; HMGX1 ; HMGXB1; Hypothetical protein FLJ10739; iBRAF; Inhibitor of BRAF35
Species
Homo sapiens (Human)
Expression Region
1-347
Target Protein Sequence
MENLMTSSTL PPLFADEDGS KESNDLATTG LNHPEVPYSS GATSSTNNPE FVEDLSQGQL LQSESSNAAE GNEQRHEDEQ RSKRGGWSKG RKRKKPLRDS NAPKSPLTGY VRFMNERREQ LRAKRPEVPF PEITRMLGNE WSKLPPEEKQ RYLDEADRDK ERYMKELEQY QKTEAYKVFS RKTQDRQKGK SHRQDAARQA THDHEKETEV KERSVFDIPI FTEEFLNHSK AREAELRQLR KSNMEFEERN AALQKHVESM RTAVEKLEVD VIQERSRNTV LQQHLETLRQ VLTSSFASMP LPGSGETPTV DTIDSYMNRL HSIILANPQD NENFIATVRE VVNRLDR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a role in neuronal differentiation as chromatin-associated protein. Acts as inhibitor of HMG20B. Overcomes the repressive effects of the neuronal silencer REST and induces the activation of neuronal-specific genes. Involved in the recruitment of the histone methyltransferase KMT2A/MLL1 and consequent increased methylation of histone H3 lysine 4.
Gene References into Functions
  1. HMG20A together with LSD1 are required for SNAI1-dependent repression of epithelial genes. PMID: 25639869
  2. ectopic expression of BRAP2 inhibits nuclear localization of HMG20A and NuMA1, and prevents nuclear envelope accumulation of SYNE2. PMID: 23707952
  3. HMG20A/iBRAF forms heterodimers with HMG20B/BRAF35. Heterodimerization impairs HMG20B/BRAF35 sumoylation and interaction with the LSD1-CoREST complex. PMID: 22570500
  4. we report associations with the LAMA1 and HMG20A (not previously associated at genome-wide significance in Europeans) gene regions with type 2 diabetes risk PMID: 22693455
Subcellular Location
Nucleus.
Tissue Specificity
Ubiquitous.
Database Links

HGNC: 5001

OMIM: 605534

KEGG: hsa:10363

STRING: 9606.ENSP00000336856

UniGene: Hs.69594

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