Recombinant Human Histone H3.1t (HIST3H3)

Code CSB-YP623092HU
MSDS
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Source Yeast
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Code CSB-EP623092HU
MSDS
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Source E.coli
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Code CSB-EP623092HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP623092HU
MSDS
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Source Baculovirus
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Code CSB-MP623092HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HIST3H3
Uniprot No.
Alternative Names
HIST3H3; H3FT; Histone H3.1t; H3/t; H3t; H3/g
Species
Homo sapiens (Human)
Expression Region
2-136
Target Protein Sequence
ARTKQTARK STGGKAPRKQ LATKVARKSA PATGGVKKPH RYRPGTVALR EIRRYQKSTE LLIRKLPFQR LMREIAQDFK TDLRFQSSAV MALQEACESY LVGLFEDTNL CVIHAKRVTI MPKDIQLARR IRGERA
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Gene References into Functions
  1. Data show that phosphatase and tensin homolog (PTEN) interacts with death domain associated protein (DAXX) and, in turn PTEN directly regulates oncogene expression by modulating DAXX-histone H3.3 (H3.3) association on the chromatin. PMID: 28497778
  2. Data suggest TCF19 interacts with histone 3 lysine 4 trimethylation through its plant homeodomain finger; TCF19 expression appears to regulate gluconeogenesis in hepatocytes; TCF19 interacts with CHD4 causing NuRD complex recruitment to gene promoters of enzymes involved in gluconeogenesis. (TCF19 = transcription factor 19; CHD4 = chromodomain helicase DNA binding protein 4; NuRD = nucleosome-remodeling-deacetylase) PMID: 29042441
  3. Data suggest that, during monocyte-into-macrophage differentiation, extensive trimethylation of histone H3 lysine 4 as well as histone H3 lysine 27 promotes occupancy of histone H3 at promoters of transcription factors such as HOXA (homeodomain proteins) and FOXO (forkhead transcription factors). PMID: 28304152
  4. Data suggest post-translational modifications of histones, trimethylation of lysine 36 in H3 (H3K36me3) and acetylation of lysine 16 in H4 (H4K16ac), have roles in DNA damage repair; H3K36me3 stimulates H4K16ac upon DNA double-strand break; SETD2, LEDGF, and KAT5 are required for these epigenetic changes. (SETD2 = SET domain containing 2; LEDGF = lens epithelium-derived growth factor; KAT5 = lysine acetyltransferase 5) PMID: 28546430
  5. Data show that tet oncogene family member 2 (TET2) cysteine-rich (CR) domain mutations disrupt the recognition of histone H3 lysine 36 (H3K36) methylation, its cellular localization, and enzyme activity. PMID: 28130413
  6. Screening for H3.3 G34 mutation should therefore be recommended as a routine diagnostic marker for supratentorial central nervous system tumors PMID: 26482474
  7. Data suggest chromatin exhibits histone 3 methylation markers (H3K4me3, H3K27me3) in fetal brain, heart, and liver, probably to keep progenitor cells in these organs ready for immediate differentiation. (study of 12-week aborted embryo tissues, China) PMID: 26719341
  8. Regulators of the histone H3-trimethyl lysine-4 (H3K4me3) mark are significantly associated with the genetic risk architecture of common neurodevelopmental disease. PMID: 26575220
  9. EP400 deposits H3.3 into chromatin alongside H2AZ and contributes to gene regulation after Pol II pre-initiation complex assembly. PMID: 26669263
  10. Concurrent acetylation and methylation at H3K27 occurs in hepatocellular carcinoma cells in association with p53 abnormalities. PMID: 24614346
  11. TIP60 interacted with H3K4me3 in response to TNF-alpha signaling. PMID: 25560918
  12. Our data highlights the complex interplay between Nrf2 and H3S10 phosphorylation in arsenite-activated HO-1 transcription. PMID: 26291278
  13. This study showed that Pediatric brainstem oligodendroglial tumors can include histone H3.3-mutated tumors and have a tendency to disseminate throughout the neuroaxis at the time of relapse. PMID: 25281433
  14. Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications. PMID: 25519718
  15. Histone H3.3. mutations drive pediatric glioblastoma through upregulation of MYCN. PMID: 23539269
  16. our data show that histone variant H3.3 occupies distinct intranuclear chromatin domains and that these genomic loci are associated with gene expression PMID: 25482197
  17. Findings suggest a role of checkpoint kinase 1 (CHK1) as an histone H3.3 serine 31 kinase. PMID: 25690891
  18. histone H3 lysine 9 acetylation has a dual role in human embryonic stem cell pluripotency and neural differentiation PMID: 25519907
  19. Posttranslational modifications of H3 histone (methylation) does not correlate with the sox2 expression in malignant gliomas. PMID: 25696994
  20. Reptin and Pontin oligomerization and activity are modulated through histone H3 N-terminal tail interaction. PMID: 25336637
  21. Deregulation of histone H3 trimethylation at lysine 27 in diffuse large B-cell lymphoma may be another high-risk phenotype PMID: 25149548
  22. Data indicate that S6 kinase 2 (S6K2) can phosphorylate histone H3 at position Thr45, which may play a role during cell proliferation and/or differentiation. PMID: 23564320
  23. Our data suggest that the modification and release of histones could serve markers of apoptosis in human cancer cells. PMID: 24952159
  24. The aim of the present study was to evaluate the prognostic value of the proliferation factors mitotic activity index (MAI), phosphohistone H3 (PPH3), cyclin B1, cyclin A and Ki67, alone and in combinations. PMID: 24324728
  25. Data suggest riboflavin (RF) status regulates hepatocyte gene expression; in HepG2 cells, depletion of LSD1 (lysine (K)-specific demethylase 1A) causes aberrant gene regulation in RF-deficient cells via changes in H3 methylation at albumin promoter. PMID: 24744315
  26. full-length PHF1 in HEK293 cells co-localizes with histone K27me3, but not with K36me3, and this co-localization depends on the trimethyllysine binding pocket indicating that K27me3 is an in vivo target for the PHF1 Tudor domain PMID: 23954330
  27. H3K4 monomethylation establishes boundaries that restrict the recruitment of chromatin-modifying enzymes to defined regions within promoters. PMID: 24656132
  28. Data indicate that MLL1 methylates Ash2L in the absence of histone H3, but only when assembled within a complex with WDR5 and RbBP5. PMID: 24235145
  29. Disruption of neocortical histone H3 homeostasis by soluble Abeta implicates Alzheimer's disease. PMID: 23582659
  30. Ki-67 staining is stronger than PHH3, making 'hot spots' easier to identify on ACIS. Ki-67 is more ideal than PHH3( Phosphohistone H3 ) for staining NENs, especially in tumors with borderline grades. PMID: 24021213
  31. histone H3 lysine 79 dimethylation (H3K79me2) and a novel identified site, H2bK5 monomethylation (H2bK5me1), were completely absent in individuals with Neural tube defects PMID: 23376398
  32. PRAME expression in leukaemic cell lines is upregulated by IFN gamma and LPS, suggesting a possible role in immune responses. Nuclear PRAME interacts with Histone H3, suggesting a role in gene regulation in the nucleus. PMID: 23460923
  33. Menin-dependent transcriptional repression of histone H3 lysine 9 methylation might play an important role in preventing tumors. PMID: 23579270
  34. Data using recombinant proteins in cell-free lysates suggest that interaction of survivin with phosphorylated histone 3 (H3T3-Phos; a mitotic biological marker appearing during cell division) is abolished by trimethylation at neighboring lysine. PMID: 23281010
  35. SIRT2-mediated H3K18 deacetylation plays a critical role during infection, which reveals an epigenetic mechanism imposed by a pathogenic bacterium to reprogram its host. PMID: 23908241
  36. The SUV39H1 chromodomain was shown to recognize histone H3K9me2/3 specifically. PMID: 23285239
  37. Results suggest that histone modification in H3K27 detected using immunohistochemistry can be successfully used as an independent prognostic factor for colorectal cancer patients with metachronous liver metastasis. PMID: 23523318
  38. C-kinase-activated protein phosphatase (CPI)-17 inhibitor knockdown in human pancreatic cancer cells results in dephosphorylation of histone H3. PMID: 23541585
  39. Results suggest that GLP may play a significant role in the maintenance of HIV-1 latency by catalyzing dimethylation of H3K9. PMID: 23541084
  40. Study shows that H3K4me3-TAF3 interactions direct global TFIID recruitment to active genes, some of which are p53 targets. Further analyses show that H3K4me3 enhances p53-dependent transcription by stimulating preinitiation complex formation. PMID: 23452851
  41. Histone H3 Ser-10 phosphorylation can be designated a new 'apoptotic histone code' mediated by PKCdelta. PMID: 22984491
  42. Histone H3 is increased during amino acid response that is associated with active transcription. PMID: 22978410
  43. The levels of H3K4me3 and H3K27me3 show dynamic changes during human oocyte maturation and preimplantation embryonic development. PMID: 22818287
  44. Increased levels of decondensed chromatin in both normal progenitor cells and cancer cells are associated with global loss of H3K27me3, which is linked to MYC overexpression. PMID: 22713676
  45. Elongated telomeres show increased trimethylated histone H3 Lys9 (H3K9me3)density. PMID: 22922742
  46. 17beta-estradiol stimulation induces the recruitment of PAD2 to target promoters by ERalpha, whereby PAD2 then citrullinates H3R26, which leads to local chromatin decondensation and transcriptional activation. PMID: 22853951
  47. Trimethylation of lysine 27 on histone H3 expression, as examined by immunohistochemistry, has the potential to be used as an immunomarker to predict nasopharyngeal carcinoma chemoradiotherapy response and patient prognosis. PMID: 21738951
  48. aberration of the global H3K9me2 level is an important epigenetic event in colorectal tumorigenesis and carcinogenesis PMID: 21917293
  49. our data suggest that global histone H3 and H4 modification patterns are potential markers of tumor recurrence and disease-free survival in non-small cell lung cancer PMID: 22360506
  50. O-GlcNAcylation regulates mitosis-specific phosphorylations on H3, providing a mechanistic switch that orchestrates the G2-M transition of the cell cycle. PMID: 22371497

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Subcellular Location
Nucleus. Chromosome.
Protein Families
Histone H3 family
Tissue Specificity
Expressed in testicular cells.
Database Links

HGNC: 4778

OMIM: 602820

KEGG: hsa:8290

STRING: 9606.ENSP00000355657

UniGene: Hs.248171

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