Recombinant Human Histone-lysine N-methyltransferase ASH1L (ASH1L), partial

Code CSB-YP865114HU
MSDS
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Source Yeast
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Code CSB-EP865114HU
MSDS
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Source E.coli
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Code CSB-EP865114HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP865114HU
MSDS
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Source Baculovirus
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Code CSB-MP865114HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ASH1L
Uniprot No.
Alternative Names
Absent small and homeotic disks protein 1 homolog; Absent Small or Homeotic Like ; Absent Small or Homeotic Like; ASH 1; ASH 1L; ash1 (absent small or homeotic 1); Ash1 (absent small or homeotic) like; ASH1-like protein; ASH1L; ASH1L_HUMAN; ASH1L1; FLJ10504; huASH1; KIAA1420; KMT2H / ASH1; Lysine N-methyltransferase 2H; Probable histone-lysine N-methyltransferase ASH1L
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Histone methyltransferase specifically trimethylating 'Lys-36' of histone H3 forming H3K36me3. Also monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro. The physiological significance of the H3K9me1 activity is unclear.
Gene References into Functions
  1. DDB2 promotes cyclobutane pyrimidine dimer excision by recruiting the histone methyltransferase ASH1L, which methylates lysine 4 of histone H3. In turn, methylated H3 facilitates the docking of the XPC complex to nucleosomal histone octamers. PMID: 29109511
  2. suppresses matrix metalloproteinase through mitogen-activated protein kinase signaling pathway in pulpitis PMID: 28041684
  3. There has been increasing evidence that heterozygous mutation of ASH1L is associated with ID and autism spectrum disorders. We suggest that ASH1L abnormalities may cause a novel MCA/ID syndrome. PMID: 28394464
  4. Epigenetic regulators play vital roles in cancer pathogenesis and represent a new frontier in therapeutic targeting. Our studies provide basic mechanistic insight into the role of H3K36me2 in transcription activation and MLL leukemia pathogenesis and implicate ASH1L histone methyltransferase as a promising target for novel molecular therapy. PMID: 27154821
  5. Our data suggest a role for ASH1L, a methyl transferase protein and member of the trithorax (Trx) family, in regulation of the HBB gene and as a potential modifier of beta-thalassaemia severity. PMID: 27434206
  6. Structural features were identified in ASH1L related to its' function and enzymatic activity. PMID: 26292256
  7. Both ASH1L and SETD2 are H3K36 specific methyltransferases but only SETD2 can trimethylate this mark. PMID: 26002201
  8. These data demonstrate that miR-142-3p downregulation has a role in thyroid tumorigenesis, by regulating ASH1L and MLL1. PMID: 25238203
  9. Our results uncover a novel regulatory cascade orchestrated by Ash1l with RAR and provide insights into mechanisms underlying the establishment of the transcriptional activation that counteracts Polycomb silencing PMID: 24244179
  10. all of the H3K36-specific methyltransferases, including ASH1L, HYPB, NSD1, and NSD2 were inhibited by ubH2A, whereas the other histone methyltransferases, including PRC2, G9a, and Pr-Set7 were not affected by ubH2A. PMID: 24019522
  11. induction of Cdk5 activity is a novel mechanism through which hASH1 may regulate migration in lung carcinogenesis PMID: 22696682
  12. Long Non-coding RNA, DBE-T recruits the Trithorax group protein Ash1L to the FSHD locus, driving histone H3 lysine 36 dimethylation, chromatin remodeling, and 4q35 gene transcription. PMID: 22541069
  13. ASH1 and MLL1 synergize in activation of Hox genes. PMID: 22140534
  14. human ASH1L specifically methylates histone H3 Lys-36. PMID: 21239497
  15. These data suggest that ASH1L occupies most, if not all, active genes and methylates histone H3 in a nonredundant fashion at a subset of genes. PMID: 17923682

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Subcellular Location
Nucleus. Cell junction, tight junction. Chromosome.
Protein Families
Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, SET2 subfamily
Tissue Specificity
Widely expressed, with highest level in brain, heart and kidney.
Database Links

HGNC: 19088

OMIM: 607999

KEGG: hsa:55870

STRING: 9606.ENSP00000376204

UniGene: Hs.491060

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