Recombinant Human Homeobox protein Nkx-2.3 (NKX2-3)

Code CSB-YP848391HU
MSDS
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Source Yeast
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Code CSB-EP848391HU
MSDS
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Source E.coli
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Code CSB-EP848391HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP848391HU
MSDS
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Source Baculovirus
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Code CSB-MP848391HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NKX2-3
Uniprot No.
Alternative Names
NKX2-3; NKX23; NKX2CHomeobox protein Nkx-2.3; Homeobox protein NK-2 homolog C
Species
Homo sapiens (Human)
Expression Region
1-364
Target Protein Sequence
MMLPSPVTST PFSVKDILNL EQQHQHFHGA HLQADLEHHF HSAPCMLAAA EGTQFSDGGE EDEEDEGEKL SYLNSLAAAD GHGDSGLCPQ GYVHTVLRDS CSEPKEHEEE PEVVRDRSQK SCQLKKSLET AGDCKAAEES ERPKPRSRRK PRVLFSQAQV FELERRFKQQ RYLSAPEREH LASSLKLTST QVKIWFQNRR YKCKRQRQDK SLELGAHAPP PPPRRVAVPV LVRDGKPCVT PSAQAYGAPY SVGASAYSYN SFPAYGYGNS AAAAAAAAAA AAAAAAYSSS YGCAYPAGGG GGGGGTSAAT TAMQPACSAA GGGPFVNVSN LGGFGSGGSA QPLHQGTAAG AACAQGTLQG IRAW
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor.
Gene References into Functions
  1. Studies suggest oncogenic NK2 homeobox 3 homeobox protein (NKX2-3) in lymphomagenesis, and show a mouse model for studying the biology and therapy of human marginal-zone B-cell lymphomas. PMID: 27297662
  2. NKX2-3 is a key mediator for maintaining myofibroblast characteristics, and our results show that colorectal myofibroblasts, as defined by the expression of AOC3, NKX2-3, and other markers, are a distinctly different cell type from TGFbeta-activated fibroblasts PMID: 27036009
  3. The persons with a NKX2-3 G or T allele may have a moderately increased risk of CD. PMID: 24473197
  4. NKX2-3 may play different roles in ulcerative colitis and Crohn's disease pathogenesis by differential regulation of EGR1. PMID: 21968973
  5. A positive correlation was observed between mRNA expression of PTPN2 and NKX2-3 in B cells and in intestinal tissues from both Crohn's disease and ulcerative colitis patients. PMID: 22377701
  6. The binding of NFAT1 to the NKX2-3 promoter region with rs1190140 was confirmed by chromatin immunoprecipitation assay. PMID: 21803625
  7. the risk haplotype of NKX2.3 confers susceptibility to UC through increasing expression of NKX2.3 mRNA in the colonic mucosa. PMID: 21514341
  8. NKX2-3 may play an important role in inflammatory bowel disease pathogenesis by regulating endothelin-1 and VEGF signaling in human intestinal microvascular endothelial cells. PMID: 21637825
  9. Data suggest that NKX2-3 and IRGM are susceptibility loci for inflammatory bowel disease in Eastern European patients. PMID: 21049557
  10. Nkx2-3 may contribute to the pathogenesis of inflammatory bowel disease-associated colorectal cancer and sporadic colorectal cancer by regulating the Wnt signaling pathway. PMID: 20165982
  11. a decrease in Nkx2-3 gene expression level can profoundly alter the expression of genes and cellular functions relevant to the pathogenesis and progression of inflammatory bowel disease, such as EDN1. PMID: 20188614
  12. Single nucleotide polymorphism in NKX2-3 gene is associated with Crohn's disease and ulcerative colitis PMID: 18438405
  13. A positive association signal presumably common to different ethnic groups for rs10883365 was detected in the upstream region of NKX2-3. PMID: 18936107

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Subcellular Location
Nucleus.
Protein Families
NK-2 homeobox family
Database Links

HGNC: 7836

OMIM: 606727

KEGG: hsa:159296

STRING: 9606.ENSP00000342828

UniGene: Hs.243272

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