Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

HAO2

Uniprot No.

Q9NYQ3

Research Area

Tags & Cell Markers

Alternative Names

(S)-2-hydroxy-acid oxidase; (S)-2-hydroxy-acid oxidase, peroxisomal; 2 hydroxy acid oxidase peroxisomal; Cell growth-inhibiting gene 16 protein; GIG16; Glycolate oxidase; HAO 2; Hao2; HAOX2; HAOX2_HUMAN; Hydroxyacid oxidase 2 (long chain); Hydroxyacid oxidase 2; Long chain alpha hydroxy acid oxidase; Long chain alpha-hydroxy acid oxidase; Long chain L 2 hydroxy acid oxidase ; Long-chain L-2-hydroxy acid oxidase; peroxisomal

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

2-351aa

Target Protein Sequence

SLVCLTDFQAHAREQLSKSTRDFIEGGADDSITRDDNIAAFKRIRLRPRYLRDVSEVDTRTTIQGEEISAPICIAPTGFHCLVWPDGEMSTARAAQAAGICYITSTFASCSLEDIVIAAPEGLRWFQLYVHPDLQLNKQLIQRVESLGFKALVITLDTPVCGNRRHDIRNQLRRNLTLTDLQSPKKGNAIPYFQMTPISTSLCWNDLSWFQSITRLPIILKGILTKEDAELAVKHNVQGIIVSNHGGRQLDEVLASIDALTEVVAAVKGKIEVYLDGGVRTGNDVLKALALGAKCIFLGRPILWGLACKGEHGVKEVLNILTNEFHTSMALTGCRSVAEINRNLVQFSRL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

54.7kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Human HAO2 protein expression in e.coli cells requires the insertion of a DNA fragment encoding the Human HAO2 protein (2-351aa) into a plasmid vector and the transferral of this vector into e.coli cells. The positive cells are screened, cultured, and then induced to express the HAO2 protein. The protein carries a N-terminal 6xHis-SUMO tag. The cells are lysed to harvest the recombinant Human HAO2 protein, which is purified through affinity purification and then detected by SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of this recombinant Human HAO2 protein is over 85%.

Customer Reviews and Q&A

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■ Q&A

Have any of your HAO2 purified proteins been assayed for activity?

Very nice to receive your inquiry.
Sorry, we haven't expressed the activity of these proteins. For the time being, we can't find any literature about the activity of its exogenous expression proteins.
There is one literature introducing the activity of the protein expressed in vivo, https://www.ncbi.nlm.nih.gov/pubmed/10777549
What we provide is the full length protein, we recommend the protein expressed by Mammalian cell expression system, the host cell is HEK293.
It has a higher post-translational modification mechanism and the structure is very close to the natural protein, so it is most likely to be active.

Target Background

Function

Catalyzes the oxidation of L-alpha-hydroxy acids as well as, more slowly, that of L-alpha-amino acids.

Gene References into Functions

Dysregulation of HAO2 is a very early event in the development of hepatocellular carcinoma (HCC). PMID: 26658681

Subcellular Location

Peroxisome.

Protein Families

FMN-dependent alpha-hydroxy acid dehydrogenase family

Tissue Specificity

Liver and kidney.

Database Links

HGNC: 4810

OMIM: 605176

KEGG: hsa:51179

STRING: 9606.ENSP00000316339

UniGene: Hs.659767

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