Recombinant Human Importin-5 (IPO5), partial

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

IPO5

Uniprot No.

Alternative Names

DKFZp686O1576; FLJ43041; IMB3; Imp5; Importin 5; Importin beta 3 subunit; Importin subunit beta 3; Importin subunit beta-3; Importin-5; IPO 5; IPO5; IPO5_HUMAN; Karyopherin beta 3; Karyopherin beta-3; KPNB3; MGC2068; Pse1; Ran binding protein 5; Ran GTP binding protein 5; Ran-binding protein 5; RanBP 5; RanBP5

Species

Homo sapiens (Human)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. Binds to CPEB3 and mediates its nuclear import following neuronal stimulation. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.

Gene References into Functions

Results indicate that the interaction between FLIL33 and IPO5 is localized to a specific segment of the FLIL33 protein, is not required for nuclear localization of FLIL33, and protects FLIL33 from proteasome-dependent degradation. PMID: 29127199
importin-alpha5, which is a key regulator of interferon signaling following Ebola virus infection, as one putative target of miRNA. PMID: 27094387
The nuclear importin IPO5 was identified as a novel interacting protein of SMAD1. Overexpression of IPO5 in various cell lines specifically increases nuclear localization of BMP receptor-activated SMADs (R-SMADs) confirming a functional relationship between IPO5 and BMP but not TGF-beta R-SMADs. PMID: 27703004
Single nucleotide polymorphism in IPO5 gene is associated with Peripheral Arterial Disease. PMID: 26488411
IQGAP1 interacts with human importin-beta5 in HEK 293T cells. PMID: 24196961
The N-terminal of influenza A virus PB1 mediates its binding to host RanBP5. PMID: 21562121
The results of this study suggested that abnormal expression and alternative splicing of the IPO5 gene may be involved in the pathophysiology of schizophrenia. PMID: 20542336
importin beta3 is essential for the nuclear import of RPL7. The import is mediated via the multifaceted basic amino acid clusters present in the NH(2)-region of RPL7, and is RanGTP-dependent PMID: 20828572
L1 major capsid protein of human papillomavirus type 11 interacts with Kap beta3 nuclear import receptors PMID: 12620808
The KPNB3 locus may contain a disease-causing variant for schizophrenia. PMID: 15364420
RanBP5 acts as an import factor for the newly synthesized influenza A virus RNA-dependent-RNA polymerase by targeting the PB1-PA dimer to the nucleus. PMID: 17005651
Lack of association of the KPNB3 locus in schizophrenia. PMID: 18295457
HPV-16 E5 protein binds to karyopherin beta3. PMID: 18455505
These results suggest that karyopherin beta3 plays a crucial role in apo A-I secretion. PMID: 18562802
Impaired p53 binding to importin: a novel mechanism of cytoplasmic sequestration identified in oxaliplatin-resistant cells. PMID: 19581934

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Subcellular Location

Cytoplasm. Nucleus. Nucleus, nucleolus. Note=Nucleus; nuclear rim. Found particularly in the nuclear rim and nucleolus.

Protein Families

Importin beta family, Importin beta-3 subfamily

Database Links

HGNC: 6402

OMIM: 602008

KEGG: hsa:3843

STRING: 9606.ENSP00000261574

UniGene: Hs.712598

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Recombinant Human Importin-5 (IPO5), partial

Product Details

Customer Reviews and Q&A

Target Background

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