Recombinant Human Inner centromere protein (INCENP), partial

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

INCENP

Uniprot No.

Alternative Names

binds and activates aurora B and C in vivo and in vitro; Chromosomal passenger protein; INCE_HUMAN; INCENP; Inner centromere protein; Inner centromere protein antigens 135/155kDa; Inner centromere protein antigens 135kD 155kD; Inner centromere protein INCENP

Species

Homo sapiens (Human)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Acts as a scaffold regulating CPC localization and activity. The C-terminus associates with AURKB or AURKC, the N-terminus associated with BIRC5/survivin and CDCA8/borealin tethers the CPC to the inner centromere, and the microtubule binding activity within the central SAH domain directs AURKB/C toward substrates near microtubules. The flexibility of the SAH domain is proposed to allow AURKB/C to follow substrates on dynamic microtubules while ensuring CPC docking to static chromatin. Activates AURKB and AURKC. Required for localization of CBX5 to mitotic centromeres. Controls the kinetochore localization of BUB1.

Gene References into Functions

Aurora-C interactions with members of the Chromosome Passenger Complex (CPC), Survivin and Inner Centromere Protein (INCENP) in reference to known Aurora-B interactions to understand the functional significance of Aurora-C overexpression in human cancer cells, is reported. PMID: 27332895
The data suggest that INCENP in the chromosomal passenger complex pathway contributes to estrogen receptor-negative breast cancer susceptibility in the European population. PMID: 25586992
Our results provide the structural basis and energetics of the human Aurora-A(G198N) - INCENP complex PMID: 23848594
v-Src induces the failure of cytokinesis and the delocalization of Mklp1, Aurora B, and INCENP from the spindle midzone. PMID: 23562843
HP1alpha binding by INCENP or Shugoshin 1 (Sgo1) is dispensable for centromeric cohesion protection during mitosis of human cells, but might regulate yet unknown interphase functions of the chromosome passenger complex (CPC) or Sgo1 at the centromeres. PMID: 21346195
Results indicate that INCENP-Aurora B localized at centromeres/inner kinetochores is sufficient to mediate SAC activity upon spindle disruption. PMID: 20372054
Dephosphorylation of INCENP at anaphase and the concomitant relocation of the chromosomal passenger protein complex prevents kinetochore recruitment of mitotic checkpoint proteins. PMID: 20619651
Data show that binding to INCENP is alone critical to the distinct function of Aurora B, and although G198 of Aurora A is required for TPX2 binding, N142G Aurora B retains INCENP binding and Aurora B function. PMID: 19494039
Aurora B kinase activity is stimulated by INCENP and C-terminal region of INCENP is sufficient for activation PMID: 12925766
association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C PMID: 15316025
Recruitment of MKLP1 to the midzone/midbody by INCENP is a crucial step for the midbody formation and completion of cytokinesis in mammalian cells. PMID: 15796717
Aurora-C is a chromosomal passenger protein that disrupts the association of INCENP with Aurora-B and may serve as a key regulator in cell division PMID: 15917996
Data show that INCENP has an important role in stabilizing the chromosomal passenger complex, and that Borealin acts to promote binding of Survivin to INCENP. PMID: 16239925
INCENP phosphorylation by Cdk1 is necessary for the recruitment of Plk1 to the kinetochore. PMID: 16378098
A functional module within the chromosomal passenger complex involving the inner centromere protein INCENP, Survivin, and Borealin. PMID: 16571674
protein truncation and in vitro mutagenesis,have identified the nucleolar localization sequences on INCENP PMID: 17623812
Borealin and INCENP associate with the helical domain of Survivin to form a tight three-helical bundle. PMID: 17956729
High INCENP expression is associated with high grade non-Hodgkin B-cell lymphomas. PMID: 18752045

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Subcellular Location

Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Midbody. Chromosome, centromere, kinetochore.

Protein Families

INCENP family

Database Links

HGNC: 6058

OMIM: 604411

KEGG: hsa:3619

STRING: 9606.ENSP00000378295

UniGene: Hs.142179

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Recombinant Human Inner centromere protein (INCENP), partial

Product Details

Customer Reviews and Q&A

Target Background

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