Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Signal Transduction
Alternative Names
1D myo inositol trisphosphate 3 kinase B; 4; 5-trisphosphate 3-kinase B; Inositol 1 4 5 trisphosphate 3 kinase B; Inositol 1; Inositol trisphosphate 3 kinase B; Inositol-trisphosphate 3-kinase B; InsP 3-kinase B; IP3 3 kinase; IP3 3 kinase B; IP3 3-kinase B; IP3K; IP3K B; IP3KB; IP3KB_HUMAN; Itpkb
Species
Homo sapiens (Human)
Expression Region
442-946aa
Target Protein Sequence
RVEGGSPTLGLLGGSPSAQPGTGNVEAGIPSGRMLEPLPCWDAAKDLKEPQCPPGDRVGVQPGNSRVWQGTMEKAGLAWTRGTGVQSEGTWESQRQDSDALPSPELLPQDPDKPFLRKACSPSNIPAVIITDMGTQEDGALEETQGSPRGNLPLRKLSSSSASSTGFSSSYEDSEEDISSDPERTLDPNSAFLHTLDQQKPRVSKSWRKIKNMVHWSPFVMSFKKKYPWIQLAGHAGSFKAAANGRILKKHCESEQRCLDRLMVDVLRPFVPAYHGDVVKDGERYNQMDDLLADFDSPCVMDCKMGIRTYLEEELTKARKKPSLRKDMYQKMIEVDPEAPTEEEKAQRAVTKPRYMQWRETISSTATLGFRIEGIKKEDGTVNRDFKKTKTREQVTEAFREFTKGNHNILIAYRDRLKAIRTTLEVSPFFKCHEVIGSSLLFIHDKKEQAKVWMIDFGKTTPLPEGQTLQHDVPWQEGNREDGYLSGLNNLVDILTEMSQDAPLA
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
This Human ITPKB recombinant protein was produced in E.coli, where the gene sequence encoding Human ITPKB (442-946aa) was expressed with the N-terminal 6xHis-SUMO tag. The purity of this ITPKB protein was greater than 90% by SDS-PAGE.
ITPKB is an enzyme, and its primary function is to phosphorylate inositol trisphosphate (IP3), converting it into inositol tetrakisphosphate (IP4). IP3 and IP4 are important cellular signaling molecules that play a crucial role in the regulation of intracellular calcium ions (Ca2+). In particular, IP3 is considered a trigger for the release of intracellular Ca2+. ITPKB is involved in intracellular calcium ion signaling pathways by regulating the concentrations of IP3 and IP4. These signaling pathways are essential for various cellular processes, including cell proliferation, differentiation, migration, and apoptosis. ITPKB is also believed to be associated with cell cycle regulation. Research has shown that the activity of ITPKB can be influenced by cell cycle regulatory proteins, thereby affecting cell division and proliferation.