Recombinant Human Interactor protein for cytohesin exchange factors 1 (IPCEF1)

Code CSB-YP840994HU
MSDS
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Source Yeast
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Code CSB-EP840994HU
MSDS
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Source E.coli
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Code CSB-EP840994HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP840994HU
MSDS
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Source Baculovirus
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Code CSB-MP840994HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
IPCEF1
Uniprot No.
Alternative Names
ICEF1_HUMAN; Interaction protein for cytohesin exchange factors 1; Interactor protein for cytohesin exchange factors 1; Ipcef1; Phosphoinositide binding protein PIP3 E; Phosphoinositide binding protein PIP3E; Phosphoinositide-binding protein PIP3-E; RP3402L9
Species
Homo sapiens (Human)
Expression Region
1-437
Target Protein Sequence
MTSYMAIDGS ALVPLRQKPR RKTQGFLTMS RRRISCKDLG HADCQGWLYK KKEKGSFLSN KWKKFWVILK GSSLYWYSNQ MAEKADGFVN LPDFTVERAS ECKKKHAFKI SHPQIKTFYF AAENVQEMNV WLNKLGSAVI HQESTTKDEE CYSESEQEDP EIAAETPPPP HASQTQSLTA QQASSSSPSL SGTSYSFSSL ENTVKTPSSF PSSLSKERQS LPDTVNSLSA AEDEGQPITF AVQVHSPVPS EAGIHKALEN SFVTSESGFL NSLSSDDTSS LSSNHDHLTV PDKPAGSKIM DKEETKVSED DEMEKLYKSL EQASLSPLGD RRPSTKKELR KSFVKRCKNP SINEKLHKIR TLNSTLKCKE HDLAMINQLL DDPKLTARKY REWKVMNTLL IQDIYQQQRA SPAPDDTDDT PQELKKSPSS PSVENSI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Enhances the promotion of guanine-nucleotide exchange by PSCD2 on ARF6 in a concentration-dependent manner.
Gene References into Functions
  1. CNK3 and IPCEF1 produce a single protein that is required for HGF dependent Arf6 activation and migration. PMID: 22085542
  2. protein-protein interaction mediated by ARNO coiled-coil domain required for ARNO induced motility; coiled-coil domain promotes assembly of multiprotein complex containing ARNO and Dock180; assembly of complex requires coiled-coil domain, GRASP and IPCEF PMID: 20016009
  3. IPCEF1 increases the in vitro and in vivo stimulation of ARFGTP formation by cytohesin 2 PMID: 12920129
  4. PTEN may down-regulate the expression of PIP3, and then down-regulate the expression of cyclin D1, which leads to the suppression of cell growth. PMID: 17067457
Subcellular Location
Cytoplasm. Cell membrane.
Database Links

HGNC: 21204

KEGG: hsa:26034

STRING: 9606.ENSP00000356189

UniGene: Hs.146100

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