Recombinant Human Intraflagellar transport protein 80 homolog (IFT80)

Code CSB-YP885802HU
MSDS
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Source Yeast
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Code CSB-EP885802HU
MSDS
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Source E.coli
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Code CSB-EP885802HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP885802HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP885802HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
IFT80
Uniprot No.
Alternative Names
ATD2; Ift80; IFT80_HUMAN; Intraflagellar transport 80 homolog (Chlamydomonas); Intraflagellar transport protein 80 homolog; KIAA1374; WD repeat domain 56; WD repeat-containing protein 56; WDR56
Species
Homo sapiens (Human)
Expression Region
1-777
Target Protein Sequence
MRLKISLLKE PKHQELVSCV GWTTAEELYS CSDDHQIVKW NLLTSETTQI VKLPDDIYPI DFHWFPKSLG VKKQTQAESF VLTSSDGKFH LISKLGRVEK SVEAHCGAVL AGRWNYEGTA LVTVGEDGQI KIWSKTGMLR STLAQQGTPV YSVAWGPDSE KVLYTAGKQL IIKPLQPNAK VLQWKAHDGI ILKVDWNSVN DLILSAGEDC KYKVWDSYGR PLYNSQPHEH PITSVAWAPD GELFAVGSFH TLRLCDKTGW SYALEKPNTG SIFNIAWSID GTQIAGACGN GHVVFAHVVE QHWEWKNFQV TLTKRRAMQV RNVLNDAVDL LEFRDRVIKA SLNYAHLVVS TSLQCYVFST KNWNTPIIFD LKEGTVSLIL QAERHFLLVD GSSIYLYSYE GRFISSPKFP GMRTDILNAQ TVSLSNDTIA IRDKADEKII FLFEASTGKP LGDGKFLSHK NEILEIALDQ KGLTNDRKIA FIDKNRDLCI TSVKRFGKEE QIIKLGTMVH TLAWNDTCNI LCGLQDTRFI VWYYPNTVYV DRDILPKTLY ERDASEFSKN PHIVSFVGNQ VTIRRADGSL VHISITPYPA ILHEYVSSSK WEDAVRLCRF VKEQTMWACL AAMAVANRDM TTAEIAYAAI GEIDKVQYIN SIKNLPSKES KMAHILLFSG NIQEAEIVLL QAGLVYQAIQ ININLYNWER ALELAVKYKT HVDTVLAYRQ KFLETFGKQE TNKRYLHYAE GLQIDWEKIK AKIEMEITKE REQSSSSQSS KSIGLKP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of the intraflagellar transport (IFT) complex B, which is essential for the development and maintenance of motile and sensory cilia.
Gene References into Functions
  1. Mutations in IFT80 can be responsible for a lethal form of short-rib polydactyly and provide the molecular basis for the Jeune-Verma-Naumoff dysplasia spectrum. PMID: 19648123
  2. Mutations in IFT80 is associated with Jeune asphyxiating thoracic dystrophy PMID: 17468754
  3. Identification and characterization of a human IFT80 long isoform (namely IFT80-L), the carboxyl terminus of which shares the protein sequence of IFT80, is reported. PMID: 18601909
Involvement in disease
Short-rib thoracic dysplasia 2 with or without polydactyly (SRTD2)
Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, cilium axoneme.
Tissue Specificity
Isoform IFT80-L is widely expressed.
Database Links

HGNC: 29262

OMIM: 611177

KEGG: hsa:57560

STRING: 9606.ENSP00000312778

UniGene: Hs.478095

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