Recombinant Human Intraflagellar transport protein 88 homolog (IFT88), partial

Code CSB-YP618756HU
MSDS
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Source Yeast
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Code CSB-EP618756HU
MSDS
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Source E.coli
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Code CSB-EP618756HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP618756HU
MSDS
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Source Baculovirus
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Code CSB-MP618756HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
IFT88
Uniprot No.
Alternative Names
D13S1056E; DAF19; hTg737; Ift88; IFT88_HUMAN; Intraflagellar transport 88 homolog; Intraflagellar transport protein 88 homolog; MGC26259; Polaris homolog; Probe hTg737 (polycystic kidney disease; autosomal recessive); Recessive polycystic kidney disease protein Tg737 homolog; RP11-172H24.2; Tetratricopeptide repeat domain 10; Tetratricopeptide repeat protein 10; TG737; TPR repeat protein 10; TTC10
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in primary cilium biogenesis. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment.
Gene References into Functions
  1. BFGF also upregulated the mRNA and protein expression of IFT88 in primary cultured chondrocytes. PMID: 28901443
  2. Data suggest that the function of the fusion transcript crystallin, lambda 1 protein - intraflagellar transport 88 (CRYL1-IFT88) is closed to CRYL1 because it contained most of domain of CRYL1. PMID: 28489570
  3. multivariate Cox regression analyses demonstrated that Tg737 expression was an independent factor for predicting the overall survival of hepatocellular carcinoma patients PMID: 28663060
  4. MiR-548a-5p negatively regulates the tumor inhibitor gene Tg737 and promotes tumorigenesis in vitro and in vivo, indicating its potential as a novel therapeutic target for hepatocellular carcinoma. PMID: 27340352
  5. subtle regulation of IFT and associated cilia structure, tunes the wnt response controlling stem cell differentiation. PMID: 24346024
  6. this work suggests that Tg737 is involved in the invasion and migration of hepatoma cells under hypoxia, with the involvement of the polycystin-1, IL-8, and TGF-beta1 signaling pathway PMID: 22974282
  7. A mutation in IFT88 causes a hitherto unknown human ciliopathy. PMID: 22941275
  8. Data show that IFT88 depletion induces mitotic defects in human cultured cells, in kidney cells from the IFT88 mouse mutant Tg737(orpk) and in zebrafish embryos. PMID: 21441926
  9. The results indicate that loss of heterozygosity of the tumor suppressor gene Tg737 may play an important role in the carcinogenetic mechanism of liver cancer stem cells. PMID: 20300861
  10. IFT88 is a centrosomal protein regulating G1-S transition in non-ciliated cells. PMID: 17264151

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Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cell projection, cilium. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm. Cell projection, cilium, flagellum.
Tissue Specificity
Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas.
Database Links

HGNC: 20606

OMIM: 600595

KEGG: hsa:8100

STRING: 9606.ENSP00000323580

UniGene: Hs.187376

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