Recombinant Human Katanin p60 ATPase-containing subunit A-like 1 (KATNAL1)

Code CSB-YP863134HU
MSDS
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Source Yeast
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Code CSB-EP863134HU
MSDS
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Source E.coli
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Code CSB-EP863134HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863134HU
MSDS
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Source Baculovirus
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Code CSB-MP863134HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KATNAL1
Uniprot No.
Alternative Names
Katanin p60 ATPase containing subunit A like 1; Katanin p60 ATPase-containing subunit A-like 1; Katanin p60 subunit A like 1; Katanin p60 subunit A-like 1; KATL1_HUMAN; KATNAL1; MGC2599; OTTHUMP00000018194; OTTHUMP00000042415; OTTHUMP00000061637; p60 katanin like 1; p60 katanin-like 1
Species
Homo sapiens (Human)
Expression Region
1-490
Target Protein Sequence
MNLAEICDNA KKGREYALLG NYDSSMVYYQ GVMQQIQRHC QSVRDPAIKG KWQQVRQELL EEYEQVKSIV STLESFKIDK PPDFPVSCQD EPFRDPAVWP PPVPAEHRAP PQIRRPNREV RPLRKEMAGV GARGPVGRAH PISKSEKPST SRDKDYRARG RDDKGRKNMQ DGASDGEMPK FDGAGYDKDL VEALERDIVS RNPSIHWDDI ADLEEAKKLL REAVVLPMWM PDFFKGIRRP WKGVLMVGPP GTGKTMLAKA VATECGTTFF NVSSSTLTSK YRGESEKLVR LLFEMARFYA PTTIFIDEID SICSRRGTSD EHEASRRVKS ELLIQMDGVG GALENDDPSK MVMVLAATNF PWDIDEALRR RLEKRIYIPL PTAKGRAELL KINLREVELD PDIQLEDIAE KIEGYSGADI TNVCRDASLM AMRRRINGLS PEEIRALSKE ELQMPVTKGD FELALKKIAK SVSAADLEKY EKWMVEFGSA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulates microtubule dynamics in Sertoli cells, a process that is essential for spermiogenesis and male fertility. Severs microtubules in an ATP-dependent manner, promoting rapid reorganization of cellular microtubule arrays. Has microtubule-severing activity in vitro.
Gene References into Functions
  1. microdeletion 13q12.3 represents a novel clinically recognizable condition and that the microtubule severing gene KATNAL1 and the chromatin-associated gene HMGB1 are candidate genes for intellectual disability inherited in an autosomal dominant pattern. PMID: 24664804
  2. KATNAL1 gene sequence variants are not associated with azoospermia. PMID: 24913027
  3. KL1-mediated microtubule severing is utilized to generate microtubule seeds within the poles and loss of this activity alters the normal balance of motor-generated forces that determine spindle length. PMID: 20519956
Subcellular Location
Cytoplasm, cytoskeleton. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, spindle.
Protein Families
AAA ATPase family, Katanin p60 subunit A1 subfamily, A-like 1 sub-subfamily
Tissue Specificity
Expressed in testis, restricted to Sertoli cells (at protein level).
Database Links

HGNC: 28361

OMIM: 614764

KEGG: hsa:84056

STRING: 9606.ENSP00000369989

UniGene: Hs.243596

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