Recombinant Human Kelch-like protein 9 (KLHL9)

Code CSB-YP879070HU
MSDS
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Source Yeast
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Code CSB-EP879070HU
MSDS
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Source E.coli
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Code CSB-EP879070HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP879070HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP879070HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KLHL9
Uniprot No.
Alternative Names
KLHL9; KIAA1354; Kelch-like protein 9
Species
Homo sapiens (Human)
Expression Region
1-617
Target Protein Sequence
MKVSLGNGEM GVSAHLQPCK AGTTRFFTSN THSSVVLQGF DQLRIEGLLC DVTLVPGDGD EIFPVHRAMM ASASDYFKAM FTGGMKEQDL MCIKLHGVNK VGLKKIIDFI YTAKLSLNMD NLQDTLEAAS FLQILPVLDF CKVFLISGVS LDNCVEVGRI ANTYNLIEVD KYVNNFILKN FPALLSTGEF LKLPFERLAF VLSSNSLKHC TELELFKAAC RWLRLEDPRM DYAAKLMKNI RFPLMTPQDL INYVQTVDFM RTDNTCVNLL LEASNYQMMP YMQPVMQSDR TAIRSDSTHL VTLGGVLRQQ LVVSKELRMY DERAQEWRSL APMDAPRYQH GIAVIGNFLY VVGGQSNYDT KGKTAVDTVF RFDPRYNKWM QVASLNEKRT FFHLSALKGH LYAVGGRSAA GELATVECYN PRMNEWSYVA KMSEPHYGHA GTVYGGLMYI SGGITHDTFQ NELMCFDPDT DKWMQKAPMT TVRGLHCMCT VGDKLYVIGG NHFRGTSDYD DVLSCEYYSP TLDQWTPIAA MLRGQSDVGV AVFENKIYVV GGYSWNNRCM VEIVQKYDPE KDEWHKVFDL PESLGGIRAC TLTVFPPEEN PGSPSRESPL SAPSDHS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex mediates the ubiquitination of AURKB and controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis.
Gene References into Functions
  1. A unique form of early onset autosomal dominant distal myopathy is associated with a kelch-like homologue 9 mutation and interferes with normal skeletal muscle through a novel pathogenetic mechanism. PMID: 20554658
Database Links

HGNC: 18732

OMIM: 611201

KEGG: hsa:55958

STRING: 9606.ENSP00000351933

UniGene: Hs.701778

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