Code | CSB-YP012580HU |
Size | $436 |
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CSB-YP012580HU in HEPES buffer, - How does the purification of the keratin take place?
- Which buffers would be used for the purification in this case?
We used nickel column affinity chromatography for protein separation and purification. The general steps are: first equilibrate the column with buffer, load the sample, and then elute with different gradient eluents, collect the corresponding elution peaks, and detect the target bands.
The eluent used was NTA-0 buffer: 20 mM Tris-HCl, 0.5 M NaCl, containing the corresponding concentration of imidazole. You specified HEPES buffer, so we will arrange for the dialysis buffer to be shipped after passing the quality control.
By default, we proceed with regular NTA-0 buffering followed by dialysis;
Of course, we can also directly use the buffer specified by the customer as the eluent for elution.