Recombinant Human Kinesin-associated protein 3 (KIFAP3)

Code CSB-YP012347HU
MSDS
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Source Yeast
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Code CSB-EP012347HU
MSDS
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Source E.coli
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Code CSB-EP012347HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP012347HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP012347HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KIFAP3
Uniprot No.
Alternative Names
FLJ22818; KAP 3; KAP-3; KAP3; KIF3AP; KIFA3_HUMAN; Kifap3; Kinesin associated protein 3; Kinesin-associated protein 3; SMAP; Smg GDS; Smg GDS associated protein; Smg GDS-associated protein
Species
Homo sapiens (Human)
Expression Region
1-792
Target Protein Sequence
MQGEDARYLK RKVKGGNIDV HPSEKALIVH YEVEATILGE MGDPMLGERK ECQKIIRLKS LNANTDITSL ARKVVEECKL IHPSKLNEVE QLLYYLQNRR DSLSGKEKKE KSSKPKDPPP FEGMEIDEVA NINDMDEYIE LLYEDIPDKV RGSALILQLA RNPDNLEELL LNETALGALA RVLREDWKQS VELATNIIYI FFCFSSFSQF HGLITHYKIG ALCMNIIDHE LKRHELWQEE LSKKKKAVDE DPENQTLRKD YEKTFKKYQG LVVKQEQLLR VALYLLLNLA EDTRTELKMR NKNIVHMLVK ALDRDNFELL ILVVSFLKKL SIFMENKNDM VEMDIVEKLV KMIPCEHEDL LNITLRLLLN LSFDTGLRNK MVQVGLLPKL TALLGNDNYK QIAMCVLYHI SMDDRFKSMF AYTDCIPQLM KMLFECSDER IDLELISFCI NLAANKRNVQ LICEGNGLKM LMKRALKFKD PLLMKMIRNI SQHDGPTKNL FIDYVGDLAA QISNDEEEEF VIECLGTLAN LTIPDLDWEL VLKEYKLVPY LKDKLKPGAA EDDLVLEVVI MIGTVSMDDS CAALLAKSGI IPALIELLNA QQEDDEFVCQ IIYVFYQMVF HQATRDVIIK ETQAPAYLID LMHDKNNEIR KVCDNTLDII AEYDEEWAKK IQSEKFRWHN SQWLEMVESR QMDESEQYLY GDDRIEPYIH EGDILERPDL FYNSDGLIAS EGAISPDFFN DYHLQNGDVV GQHSFPGSLG MDGFGQPVGI LGRPATAYGF RPDEPYYYGY GS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in tethering the chromosomes to the spindle pole and in chromosome movement. Binds to the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex and may regulate the membrane binding of this complex.
Gene References into Functions
  1. This study does not show an influence of KIFAP3 variants on survival in the studied Swiss and Swedish cohort PMID: 28140676
  2. Common genetic variation in the Kinesin-Associated Protein 3 (KIFAP3) gene (rs1541160) was reported to have a significant effect on survival in amyotrophic lateral sclerosis patients. PMID: 24838185
  3. The incidence of the motor neuron-predominant amyotrophic lateral sclerosis phenotype in the patients of this cohort supports the hypothesis that the SNP within the KIFAP3 gene is a potential modifier of the amyotrophic lateral sclerosis phenotype PMID: 21659726
  4. Data clearly show that SNP rs1541160 does not modify survival within cohort of 504 Italian ALS cases. PMID: 20566859
  5. Kinesin-2 plays a role in the KDEL-R-/COPI-dependent retrograde transport pathway from the Golgi complex to the ER. PMID: 17113389
  6. KAP3 sequestration by misfolded SOD1 and the inhibition of ChAT transport play a role in the dysfunction of amyotrophic lateral sclerosis. PMID: 19088126
  7. a variant within the KIFAP3 gene that is associated with decreased KIFAP3 expression and increased survival in sporadic ALS was identified. PMID: 19451621

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Database Links

HGNC: 17060

OMIM: 601836

KEGG: hsa:22920

STRING: 9606.ENSP00000354560

UniGene: Hs.433442

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