Recombinant Human Kinetochore protein NDC80 homolog (NDC80)

1. Aurora A kinase regulates kinetochore-microtubule dynamics of metaphase chromosomes, and Hec1 S69, a previously uncharacterized phosphorylation target site in the Hec1 tail, is a critical Aurora A substrate for this regulation. PMID: 29187526
2. Ska complex directly binds Ndc80 complex through interactions between the Ska3 unstructured C-terminal region and the coiled-coil regions of each Ndc80 complex subunit. PMID: 29487209
3. Hec1 tail phosphorylation tunes friction along polymerizing microtubules and yet does not compromise the kinetochore's ability to grip depolymerizing microtubules. PMID: 28552353
4. We provided novel evidence that NDC80 expression is upregulated in osteosarcoma tissues, where it is positively correlated with advanced tumor stage and distant metastasis PMID: 28537682
5. Ndc80 recruits Bod1 to kinetochores which directly feeds forward to regulate Ndc80 PMID: 29142109
6. The authors show that the Astrin-SKAP complex binds synergistically to microtubules with the Ndc80 complex to form an integrated interface. PMID: 28841134
7. Elevated expression of NDC80 may play a role in promoting the development of hepatocellular carcinoma PMID: 28611520
8. Study shows that Cdk1 phosphorylates Ska3 to promote its direct binding to the Ndc80 complex (Ndc80C), a core outer kinetochore component, also show that this phosphorylation occurs specifically during mitosis and is required for the kinetochore localization of the Ska complex. PMID: 28479321
9. This study suggests that Ndc80 may play an important role in the process of hepatitis B virus-related hepatocellular carcinoma, and that it may be a potential biological treatment target in the future. PMID: 26382282
10. Whereas CENP-C recruits a single MIS12:NDC80 complex, the authors show here that CENP-T binds one MIS12:NDC80 and two NDC80 complexes upon phosphorylation by the mitotic CDK1:Cyclin B complex at three distinct CENP-T sites. PMID: 28012276
11. Ska is recruited to kinetochores by clusters of Ndc80 proteins. PMID: 28535377
12. Expression of NDC80 in colon cancer cells and tissues was higher than that in controls. NDC80 promotes the proliferation and metastasis of colon cancer cells. PMID: 27173328
13. Ndc80 complex bound to microtubules binds every tubulin monomer along the microtubule protofilament. PMID: 26941333
14. we conclude that Hec1 is consistently overexpressed in human PCa and Hec1 is closely linked with human PCa progression through the meditator LncRNA BX647187 PMID: 26612002
15. Independent molecular binding events to microtubules (MTs) by individual NDC80 complexes, rather than their structured oligomers, regulate the dynamics and stability of kinetochore-MT attachments in dividing cells. PMID: 25808492
16. The Ndc80 complex binds straight microtubules by recognizing the dimeric interface of tubulin. PMID: 24413531
17. Overproduction of Ndc80 in cancer cells may unfavourably absorb protein interactors through the internal loop domain and lead to a change in the equilibrium of microtubule-associated proteins. [Review] PMID: 25557589
18. N-terminus-modified Hec1 suppresses tumour growth by interfering with kinetochore-microtubule dynamics PMID: 25132262
19. Mechanisms of mitosis-specific assembly of the checkpoint platform Knl1/MIS12/NDC80 at human kinetochores. PMID: 25601404
20. Certain clinical subtypes of breast cancer more likely to respond to Hec1-targeted therapy were identified and these subtypes are the ones associated with poor prognosis. PMID: 24694948
21. Hec1 is critical in maintaining the in vitro and in vivo growth of gastric cancer cells; elevated Hec1 levels may occur at the early stage of gastric tumorigenesis PMID: 23591767
22. Growth inhibition following knockdown of NDC80, CDK1 and PLK1. PMID: 24327015
23. Hec1 expression was highest in paclitaxel-resistant A2780/Taxol cells. PMID: 23474708
24. these findings demonstrated that three buried glutamic acid-lysine pairs, in concert with hydrophobic interactions of core residues, provide the major specificity and stability requirements for Hec1-Nuf2 dimerization PMID: 24129578
25. a novel role for Aurora B-Hec1-Mps1 signaling axis in governing accurate chromosome segregation in mitosis PMID: 24187132
26. NDC80, NUF2 and PTN were significantly aberrantly overexpressed in serous adenocarcinomas. PMID: 23056589
27. Ndc80's interaction with either growing or shrinking microtubule ends can be tuned by the phosphorylation state of its tail. PMID: 23085714
28. The Ndc80 internal loop is essential for end-on microtubule attachment to kinetochores. PMID: 22454517
29. The Ndc80 kinetochore complex directly modulates microtubule dynamics. PMID: 22908300
30. results reveal the molecular function of CENP-T proteins and demonstrate how the Ndc80 complex is anchored to centromeres in a manner that couples chromosome movement to spindle dynamics PMID: 22561346
31. results support the conclusion that Cdt1 binding to Hec1 is essential for an extended Ndc80 configuration and stable kinetochore-microtubule attachment PMID: 22581055
32. Hec1 serine 165 (S165) is preferentially phosphorylated at kinetochores by Nek2 and it serves as an important mechanism in modulating spindle assemble checkpoint signaling and chromosome alignment. PMID: 21832156
33. In vertebrates there is a tripartite attachment point facilitating the interaction between Hec1/Ndc80 and microtubules. PMID: 21325630
34. N-terminally modified Hec1 promotes spindle pole fragmentation by CENP-E-mediated plus-end directed kinetochore pulling forces that disrupt the fine balance of kinetochore- and centrosome-associated forces regulating spindle bipolarity PMID: 21297979
35. These data suggest that the CH and tail domains of Hec1 generate essential contacts between kinetochores and microtubules in cells, whereas the Nuf2 CH domain does not. PMID: 21270439
36. Hec1 phosphorylation control kinetochore-microtubule attachment stability during mitosis. PMID: 21266467
37. Alteration in NDC80, were also detected in benign breast tumors. PMID: 21352579
38. Data provide evidence for a functional link between Hec1 expression and the pRb pathway. PMID: 20948316
39. CENP-U is a novel microtubule binding protein and plays an important role in kinetochore-microtubule attachment through its interaction with Hec1 PMID: 21056971
40. subnanometre-resolution cryo-electron microscopy reconstruction of the human Ndc80 complex bound to microtubules PMID: 20944740
41. Hec 1 was highhy expressed in cancer tissues with lymph node metastasis and poor differentiation. PMID: 18782526
42. Results suggest that Hec1, through cooperation with Hice1, contributes to centrosome-directed microtubule growth to facilitate establishing a proper mitotic spindle. PMID: 19776357
43. cell growths of colorectal and gastric cancers after the siRNA-mediated knockdown of either CDCA1 or KNTC2 were significantly suppressed. PMID: 19878654
44. role in spindle checkpoint signaling; required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores PMID: 12351790
45. cell cycle-regulated serine phosphorylation of Hec1 by Nek2 is essential for faithful chromosome segregation PMID: 12386167
46. HEC1 is a core component of the kinetochore outer plate essential for organizing microtubule attachment sites. PMID: 15548592
47. the Spc24, Spc25, Nuf2, and Ndc80/Hec1 complex is a faithful copy of the endogenous Ndc80 complex PMID: 15961401
48. These findings reveal a key role for the Hec1 N terminus in controlling dynamic behavior of kinetochore microtubules. PMID: 17129782
49. Study describes the crystal structure of the most conserved region of HEC1, which lies at one end of the rod and near the N terminus of the polypeptide chain, it folds into a calponin-homology domain. PMID: 17195848
50. overexpression of either CREB or ATF4 enhanced the activation of the HEC1 promoter and overexpression of both of them had an additive effect on the activation of the HEC1 transcription. PMID: 17822787

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HGNC: 16909

OMIM: 607272

KEGG: hsa:10403

STRING: 9606.ENSP00000261597

UniGene: Hs.414407