Recombinant Human Leucine-rich repeat serine/threonine-protein kinase 1 (LRRK1), partial

Code CSB-YP655748HU
MSDS
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Source Yeast
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Code CSB-EP655748HU
MSDS
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Source E.coli
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Code CSB-EP655748HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP655748HU
MSDS
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Source Baculovirus
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Code CSB-MP655748HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
LRRK1
Uniprot No.
Alternative Names
KIAA1790; Leucine rich repeat serine/threonine protein kinase 1; Leucine-rich repeat serine/threonine-protein kinase 1; LRRK 1; Lrrk1; LRRK1_HUMAN; RIPK6; Roco1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Gene References into Functions
  1. data suggest that LRRK1 variant (rs2924835) and LRRK2 variants (rs34594498, rs34410987, and rs33949390) are not associated with essential tremor in this Han Chinese population PMID: 29812962
  2. LRRK1 plays a critical role in the regulation of bone mass in humans. PMID: 27055475
  3. A novel LRRK1 mutation has been identified in two siblings in an Indian family with osteosclerotic metaphyseal dysplasia. PMID: 27829680
  4. We find that LRRK1-mediated phosphorylation of CLIP-170 causes the accumulation of p150(Glued) (also known as DCTN1) a subunit of dynactin, at microtubule plus ends, thereby facilitating the migration of EGFR-containing endosomes. PMID: 25413345
  5. LRRK1 carries out distinct functions from LRRK2 by interacting with different cellular proteins. PMID: 24947832
  6. LRRK1 mutations may have a role in Parkinson's disease PMID: 24241507
  7. The function of LRRK1 and both the physiological and the pathological roles of LRRK2 are only beginning to unfold. PMID: 22988872
  8. purified and active LRRK1 and LRRK2 can form dimers in their full-length conformation PMID: 22952686
  9. feedback down-regulation of LRRK1 kinase activity by EGFR plays an important role in the appropriate endosomal trafficking of EGFR PMID: 22337768
  10. We report the molecular characterization of human LRRK1 and show for the first time that LRRK1 is a functional protein kinase and a GDP/GTP-binding protein. We propose a model in which LRRK1 cycles between a GTP-bound active and a GDP-bound inactive state PMID: 16243488
  11. Comparison of evolutionary interspecies sequences of LRRK1 and LRRK2 suggests they diverged from a common founder gene implicated in Parkinson disease. PMID: 17225181
  12. Two rare coding variants ss65713826 and ss65713830 were more frequent in patients than controls in norwegain Parkinson's disease. PMID: 17324517
  13. mutations in LRRK2 are more prone to form inclusion bodies in transfected cells and are more toxic than equivalent mutations in LRRK1 PMID: 17394548
  14. LRRK1 genes encode low levels of expressed mRNA corresponding to low levels of protein during development. PMID: 18045479
  15. LRRK1 are active in the adult human cortex cerebri, hippocampus and also seen in the young human thymus. PMID: 18272292
  16. LRRK1 binds GTP and GDP via its Roc domain and autophosphorylation activity is selectively triggered by GTP. Kinase activity was decreased following introduction of mutations homologous to those of LRRK2 that have been associated with Parkinson's disease. PMID: 16243488

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Subcellular Location
Cytoplasm.
Protein Families
Protein kinase superfamily, TKL Ser/Thr protein kinase family, ROCO subfamily
Database Links

HGNC: 18608

OMIM: 610986

KEGG: hsa:79705

STRING: 9606.ENSP00000373600

UniGene: Hs.407918

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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