Recombinant Human Linker for activation of T-cells family member 2 (LAT2), partial

Code CSB-YP872419HU
MSDS
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Source Yeast
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Code CSB-EP872419HU
MSDS
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Source E.coli
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Code CSB-EP872419HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP872419HU
MSDS
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Source Baculovirus
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Code CSB-MP872419HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
LAT2
Uniprot No.
Alternative Names
HSPC046; LAB; LAT2; Linker for activation of B cells; Linker for activation of B-cells; Linker for activation of T cells family member 2 ; Linker for activation of T cells transmembrane adaptor 2; Linker for activation of T-cells family member 2; Membrane associated adapter molecule; Membrane-associated adapter molecule; Non T cell activation linker; Non-T-cell activation linker; NTAL_HUMAN; WBSCR 5; Wbscr15; Williams Beuren syndrome chromosome region 15; Williams Beuren syndrome chromosome region 5; Williams-Beuren syndrome chromosomal region 15 protein; Williams-Beuren syndrome chromosomal region 5 protein; WSCR5
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2.
Gene References into Functions
  1. Its overexpression represents a negative prognostic marker that has been linked to tumor grade, proliferating potential and angiogenesis and mediates intracellular transport of anticancer agents. PMID: 28815339
  2. The AML1/ETO target gene LAT2 interferes with differentiation of normal hematopoietic precursor cells. PMID: 24456692
  3. LAT2 is an early mediator of the anti-leukemic activity of alkylphospholipids and arsenic trioxide. Thus, LAT2 may be used as a target for the design of drugs for cancer therapy. PMID: 23001822
  4. NTAL acts as a tumor suppressor that enhances the proximal signaling of leukemic blasts. The key downstream molecule responsible for the biological effect of TCR signaling is ERK. PMID: 22269118
  5. LAB has the ability to modulate signaling in virtually every type of leukocyte. PMID: 20643813
  6. LAT2 protein (NTAL) participates in the activation of the c-Met-Grb2-ERK-cPLA2 signalling cascade at early stages of H. pylori infection. PMID: 19879355
  7. data show that NTAL (non-T cell activation linker) appears to be a structural and possibly also functional homologue of LAT (linker for activation of T cells)in non-T cells [non-T cell activation linker] PMID: 12486104
  8. LAB links BCR engagement to downstream signaling pathways. PMID: 12514734
  9. LAB was primarily phosphorylated on three membrane-distal tyrosines, Tyr(136), Tyr(193), and Tyr(233). Mutation of these three tyrosines abolished Grb2 binding and LAB function PMID: 14722116
  10. LAB resembled a LAT molecule unable to bind phospholipase C-gamma1. PMID: 15153499
  11. The NTAL/LAB is expressed in a variety of immune cells. PMID: 15745852
  12. NTAL acts as a negative regulator of TNF-alpha and IL-8 production after stimulation via TREM-1 PMID: 17277102
  13. observations reported herein support the conclusion that NTAL may be differentially utilized by specific receptors for relaying alternative signals and this suggests a flexibility in the function of TRAPs not previously appreciated. PMID: 17993265
  14. These data show that natural killer cell immunoreceptor tyrosine-based activation motifs preferentially use a signaling cassette regulated by interplay between LAT and LAB. PMID: 18645037
  15. expression separates T lineage leukemias into two subgroups with good and poor prognostic outcome to prednisone therapy in acute lymphoblastic leukemia in children PMID: 19183565

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Involvement in disease
LAT2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of LAT2 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
Subcellular Location
Cell membrane; Single-pass type III membrane protein. Note=Present in lipid rafts.
Tissue Specificity
Highly expressed in spleen, peripheral blood lymphocytes, and germinal centers of lymph nodes. Also expressed in placenta, lung, pancreas and small intestine. Present in B-cells, NK cells and monocytes. Absent from T-cells (at protein level).
Database Links

HGNC: 12749

OMIM: 605719

KEGG: hsa:7462

STRING: 9606.ENSP00000275635

UniGene: Hs.741249

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