Recombinant Human MMS19 nucleotide excision repair protein homolog (MMS19), partial

Code CSB-YP836306HU
MSDS
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Source Yeast
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Code CSB-EP836306HU
MSDS
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Source E.coli
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Code CSB-EP836306HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP836306HU
MSDS
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Source Baculovirus
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Code CSB-MP836306HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MMS19
Uniprot No.
Alternative Names
hMMS19; homolog of yeast MMS19; MET18; MET18 homolog; MET18MMS19 nucleotide excision repair homolog (S. cerevisiae); mms19; MMS19 nucleotide excision repair protein homolog; MMS19 nucleotide excision repair, S. cerevisiae, homolog of; MMS19-like (MET18 homolog, S. cerevisiae); MMS19-like protein; MMS19_HUMAN; MMS19L
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins such as ERCC2/XPD, FANCJ and RTEL1, thereby playing a key role in nucleotide excision repair (NER), homologous recombination-mediated double-strand break DNA repair, DNA replication and RNA polymerase II (POL II) transcription. As part of the mitotic spindle-associated MMXD complex, plays a role in chromosome segregation, probably by facilitating iron-sulfur (Fe-S) cluster assembly into ERCC2/XPD. Together with CIAO2, facilitates the transfer of Fe-S clusters to the motor protein KIF4A, which ensures proper localization of KIF4A to mitotic machinery components to promote the progression of mitosis. Indirectly acts as a transcriptional coactivator of estrogen receptor (ER), via its role in iron-sulfur insertion into some component of the TFIIH-machinery.
Gene References into Functions
  1. findings suggest that MMS19 plays an essential role in maintaining mitochondrial genome stability PMID: 29035693
  2. POLE1 is phosphorylated at serine-1940 after DNA damage and interacts with the iron-sulfur complex chaperones CIAO1 and MMS19. PMID: 27235625
  3. We therefore propose the expression level of MMS19 as a candidate predictive marker of ACT benefit in resected NSCLC patients. PMID: 27802208
  4. Suggest that MMS19 may be a potential new predictor of metastasis and chemoradiotherapy response in esophageal squamous cell carcinoma. PMID: 25892874
  5. MMS19L polymorphisms are associated with response to chemotherapy in osteosarcoma. PMID: 23886164
  6. Single nucleotide polymorphisms in the MMS19L gene is associated with bone malignant tumors. PMID: 23679317
  7. Polymorphisms in ERCC1, codon-118 and MMS19 genes are not associated with clinical response to platinum or survival. PMID: 23632208
  8. MMS19 interacts with target proteins. MIP18 has a role to bridge MMS19 and CIAO1. CIAO1 also binds IOP1. PMID: 23585563
  9. The mammalian proteins MMS19, MIP18, and ANT2 are involved in cytoplasmic iron-sulfur cluster protein assembly. PMID: 23150669
  10. study demonstrates MMS19 forms a complex with the cytoplasmic Fe-S assembly (CIA) proteins CIAO1, IOP1 and MIP18; cytoplasmic MMS19 also binds to multiple nuclear Fe-S proteins involved in DNA metabolism; propose that MMS19 functions as a platform to facilitate Fe-S cluster transfer to proteins critical for DNA replication and repair PMID: 22678361
  11. identified MMS19 as a member of the cytosolic iron-sulfur protein assembly (CIA) machinery; MMS19 functions as part of the CIA targeting complex that interacts with and facilitates iron-sulfur cluster insertion into apoproteins involved in methionine biosynthesis, DNA replication, DNA repair, and telomere maintenance PMID: 22678362
  12. Results indicate that the MMS19-XPD protein complex is required for proper chromosome segregation, an abnormality of which could contribute to the pathogenesis in some cases of xeroderma pigmentosum. PMID: 20797633
  13. Cloning of the human MMS19 genes and functional complementation in Saccharomyces cerevisiae PMID: 11328871
  14. MMS19 HEAT repeat domain is essential for MMS19 function in NER and transcription, while domains A and B, within MMS19 N-terminus, modulate the balance between DNA repair and transcription. PMID: 16797255
  15. Single nucleotide polymorphisms in MMS19L is associated with pancreatic cancer. PMID: 19318433

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Subcellular Location
Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Protein Families
MET18/MMS19 family
Tissue Specificity
Ubiquitously expressed with higher expression in testis.
Database Links

HGNC: 13824

OMIM: 614777

KEGG: hsa:64210

STRING: 9606.ENSP00000359818

UniGene: Hs.500721

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