Recombinant Human Macrophage-capping protein (CAPG)

Code CSB-EP004489HU
MSDS
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
CAPG
Uniprot No.
Research Area
Immunology
Alternative Names
Actin capping protein GCAP39; Actin regulatory protein CAP G; Actin regulatory protein CAP-G; AFCP; Capg; CAPG_HUMAN; Capping actin protein gelsolin like; Capping protein (actin filament) gelsolin like; Capping protein gelsolin like; Epididymis secretory protein Li 66; gCap39; Gelsolin like capping protein; HEL S 66; Macrophage capping protein; Macrophage-capping protein; mbh1; MCP; Myc basic motif homolog 1
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-348aa
Target Protein Sequence
MYTAIPQSGSPFPGSVQDPGLHVWRVEKLKPVPVAQENQGVFFSGDSYLVLHNGPEEVSHLHLWIGQQSSRDEQGACAVLAVHLNTLLGERPVQHREVQGNESDLFMSYFPRGLKYQEGGVESAFHKTSTGAPAAIKKLYQVKGKKNIRATERALNWDSFNTGDCFILDLGQNIFAWCGGKSNILERNKARDLALAIRDSERQGKAQVEIVTDGEEPAEMIQVLGPKPALKEGNPEEDLTADKANAQAAALYKVSDATGQMNLTKVADSSPFALELLISDDCFVLDNGLCGKIYIWKGRKANEKERQAALQVAEGFISRMQYAPNTQVEILPQGRESPIFKQFFKDWK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
65.5kDa
Protein Length
Full Length of BC014549
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.
Gene References into Functions
  1. Study suggested that CapG could be used as a biomarker for metastatic CRC in the clinical specimens. Moreover, our in vitro study demonstrated that CapG might contribute on tumor metastasis in human CRCs. PMID: 30155403
  2. Results revealed that CapG is a novel independent prognostic predictor for glioma patients and highlight a key role of CapG in proliferation and metastasis of glioma. PMID: 29399702
  3. it is demonstrated that CapG is expressed in the cytoplasm and could be used as a prognostic or diagnostic biomarker for mHCC in clinical specimens PMID: 29970516
  4. The combination of CTNB1, XPO2, and CAPG achieved 95% sensitivity and 96% specificity for the discrimination of these subtypes. We developed two uterine aspirate-based signatures to diagnose Endometrial cancer and classify tumors in the most prevalent histologic subtypes. This will improve diagnosis and assist in the prediction of the optimal surgical treatment PMID: 28790116
  5. Overexpression of CAPG is associated with glioma. PMID: 26663173
  6. On the basis of these results, we propose a model in which dynamic vimentin filaments target CARMIL2 to critical membrane-associated locations, where CARMIL2 regulates CP, and thus actin assembly, to create cell protrusions PMID: 26466680
  7. The composite biomarker, CAPG and GIPC1 in primary breast tumors, predicted disease outcomes and benefit from zoledronate and may facilitate patient selection for adjuvant bisphosphonate treatment. PMID: 26757732
  8. A single nucleotide polymorphism rs6886 inside the CapG gene was identified, affecting a CapG phosphorylation site and thus potentially modifying CapG function. PMID: 24804218
  9. CapG was up-regulated in the tumor tissues of patients with lymph node metastasis (LNM), whereas it showed an equivalent expression level between non-tumor and tumor tissues of patients without LNM. PMID: 23782053
  10. CapG is involved in the process of metastasis by promoting the invasiveness of tumor cells. PMID: 23085225
  11. CapG was identified as a novel candidate biomarker to predict response to gemcitabine treatment and survival in cholangiocarcinoma. PMID: 22155129
  12. Expression of CapG, gelsolin, and P-gp was found to be associated with an increased risk of death from non-small cell lung cancer. PMID: 22190510
  13. These results suggest that overexpression of CapG may be associated with progression of lung adenocarcinoma. PMID: 21908955
  14. strong down-regulation of MCK activity contributes to F-actin instability and induces post-translational modification of alphaB-crystallin and desmin PMID: 21768101
  15. CapG was upregulated in the stroma cells of nasopharyngeal carcinoma (NPC) compared with normal nasopharyngeal epithelial tissues. CapG possibly plays a role in the complex interaction between NPC cells and the surrounding host tissue. PMID: 19242827
  16. Proteomic study of macrophages exposed to oxLDL identifies a CAPG polymorphism associated with carotid atherosclerosis. PMID: 19439302
  17. CapG lacks a nuclear export sequence present in structurally related proteins PMID: 12637565
  18. importin-beta-dependent nuclear import of the actin modulating protein CapG promotes cell invasion PMID: 15454578
  19. CapG is a new tumor suppressor gene involved in the tumorigenic progression of certain cancers PMID: 16767159
  20. Comprehension of the mobility and compartmentalization of the CapG protein in normal and in cancer cells in vivo could constitute a new basis to characterize the invasiveness and metastasizing potential of breast cancer. PMID: 18059028
  21. Dysregulated expression of gelsolin-like actin capping protein(CapG) was found in premalignant and malignant oral carcinogenesis. PMID: 18237446
  22. Results report that NTF2 and Ran control nuclear import of the filamentous actin capping protein CapG. PMID: 18266911
  23. CapG mobility in cell nuclei of live breast cancer cells PMID: 18461482
  24. the 4 biomarkers CLU, ITGB3, PRAME and CAPG may be used as prognostic factors for patients with stage III serous ovarian adenocarcinomas. PMID: 18709641
  25. These results suggest that filamentous actin in the nucleolus might be regulated by actin binding proteins such as CapG. PMID: 18938132
  26. several genes that are known to be regulated by DNA methylation were up-regulated dramatically by integrin alpha6beta4 expression, including S100A4, FST, PDLIM4, CAPG, and Nkx2.2. PMID: 19011242
  27. A role for the actin-binding protein CapG as a mediator of cross-talk between the actin cytoskeleton and microtubule-based organelles that regulate cell division was proposed. PMID: 19166812
  28. Hypoxia-inducible factor 1alpha up-regulated CapG protein expression under normoxia. Knockdown of HIF-1alpha expression in hPASMCs also inhibited hypoxia-induced CapG up-regulation. PMID: 19188659

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Subcellular Location
Nucleus. Cytoplasm. Melanosome. Cell projection, lamellipodium. Cell projection, ruffle.
Protein Families
Villin/gelsolin family
Tissue Specificity
Macrophages and macrophage-like cells.
Database Links

HGNC: 1474

OMIM: 153615

KEGG: hsa:822

STRING: 9606.ENSP00000263867

UniGene: Hs.516155

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