Recombinant Human Male-specific lethal 1 homolog (MSL1)

Code CSB-YP731563HU
MSDS
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Source Yeast
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Code CSB-EP731563HU
MSDS
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Source E.coli
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Code CSB-EP731563HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP731563HU
MSDS
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Source Baculovirus
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Code CSB-MP731563HU
MSDS
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Source Mammalian cell
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Product Details

Purity
≥85% (SDS-PAGE)
Target Names
MSL1
Uniprot No.
Alternative Names
hMSL1; male specific lethal 1; male specific lethal 1 homolog 1; male specific lethal 1 homolog; male specific lethal 1 like 1; Male-specific lethal 1 homolog; Male-specific lethal 1-like 1; Male-specific lethal-1 homolog 1; MSL 1; MSL-1; Msl1; MSL1 like 1; MSL1-like 1; MSL1_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-614
Target Protein Sequence
MTMRSAVFKA AAAPAGGNPE QRLDYERAAA LGGPEDEPGA AEAHFLPRHR KLKEPGPPLA SSQGGSPAPS PAGCGGKGRG LLLPAGAAPG QQEESWGGSV PLPCPPPATK QAGIGGEPAA AGAGCSPRPK YQAVLPIQTG SLVAAAKEPT PWAGDKGGAA SPAATASDPA GPPPLPLPGP PPLAPTATAG TLAASEGRWK SMRKSPLGGG GGSGASSQAA CLKQILLLQL DLIEQQQQQL QAKEKEIEEL KSERDTLLAR IERMERRMQL VKKDNEKERH KLFQGYETEE REETELSEKI KLECQPELSE TSQTLPPKPF SCGRSGKGHK RKSPFGSTER KTPVKKLAPE FSKVKTKTPK HSPIKEEPCG SLSETVCKRE LRSQETPEKP RSSVDTPPRL STPQKGPSTH PKEKAFSSEI EDLPYLSTTE MYLCRWHQPP PSPLPLRESS PKKEETVARC LMPSSVAGET SVLAVPSWRD HSVEPLRDPN PSDLLENLDD SVFSKRHAKL ELDEKRRKRW DIQRIREQRI LQRLQLRMYK KKGIQESEPE VTSFFPEPDD VESLMITPFL PVVAFGRPLP KLTPQNFELP WLDERSRCRL EIQKKQTPHR TCRK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of histone acetyltransferase complex responsible for the majority of histone H4 acetylation at 'Lys-16' (H4K16ac) which is implicated in the formation of higher-order chromatin structure. Greatly enhances MSL2 E3 ubiquitin ligase activity, promoting monoubiquitination of histone H2B at 'Lys-34' (H2BK34Ub). This modification in turn stimulates histone H3 methylation at 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) and leads to gene activation, including that of HOXA9 and MEIS1. In the MSL complex, acts as a scaffold to tether MSL3 and KAT8 together for enzymatic activity regulation.
Gene References into Functions
  1. Studies identi fi ed a novel signal of nuclear localization (NLS) in all MSL1 protein isoforms and found that the combination of both NLS allows for its intra-nuclear focal accumulation and nuclear transport of TTC4 while all MSL1 isoforms affect H4K16Ac. PMID: 24913909
  2. The MSL1 bound to Nupr1, with a moderate affinity (2.8 microM) in an entropically-driven process. MSL1 did not bind to non-damaged DNA, but it bound to chemically-damaged-DNA with a moderate affinity (1.2 microM) also in an entropically-driven process. PMID: 24205110
  3. MSL1 plays an important role in mediating irradiation-induced DNA repair through formation of HAT complexes and interaction with 53BP1. P8 acts as a negative regulator of this process by interacting with MSL1 and preventing its role on HAT activity. PMID: 19650074
  4. A multisubunit human histone acetylase complex that contains homologs of the Drosophila MSL proteins MOF, MSL1 (hampin A), MSL2, and MSL3 was described. This complex is responsible for histone H4 lysine-16 acetylation of all cellular chromosomes. PMID: 16227571
Subcellular Location
Nucleus. Nucleus, nucleoplasm. Nucleus speckle.
Protein Families
Msl-1 family
Database Links

HGNC: 27905

OMIM: 614801

KEGG: hsa:339287

STRING: 9606.ENSP00000462945

UniGene: Hs.532786

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