Recombinant Human Matrilin-3 (MATN3)

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Code CSB-EP013522HU
Size US$306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
MATN3
Uniprot No.
Research Area
Others
Alternative Names
AV009181; DIPOA; EDM5; HOA; MATN3; MATN3_HUMAN; Matrilin 3; Matrilin-3; OADIP; OS2
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
29-486aa
Target Protein Sequence
DPVARPGFRRLETRGPGGSPGRRPSPAAPDGAPASGTSEPGRARGAGVCKSRPLDLVFIIDSSRSVRPLEFTKVKTFVSRIIDTLDIGPADTRVAVVNYASTVKIEFQLQAYTDKQSLKQAVGRITPLSTGTMSGLAIQTAMDEAFTVEAGAREPSSNIPKVAIIVTDGRPQDQVNEVAARAQASGIELYAVGVDRADMASLKMMASEPLEEHVFYVETYGVIEKLSSRFQETFCALDPCVLGTHQCQHVCISDGEGKHHCECSQGYTLNADKKTCSALDRCALNTHGCEHICVNDRSGSYHCECYEGYTLNEDRKTCSAQDKCALGTHGCQHICVNDRTGSHHCECYEGYTLNADKKTCSVRDKCALGSHGCQHICVSDGAASYHCDCYPGYTLNEDKKTCSATEEARRLVSTEDACGCEATLAFQDKVSSYLQRLNTKLDDILEKLKINEYGQIHR
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
57.2 kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The synthesis of the recombinant plasmid containing the gene encoding the Human MATN3 protein (29-486aa) is the first step to produce the recombinant Human MATN3 protein. After that, the recombinant plasmid is transformed into e.coli cells. e.coli cells capable of enduring a specific antibiotic are selected, demonstrating successful uptake of the recombinant plasmid. The e.coli cells containing the recombinant plasmid are cultured under conditions that encourage the expression of the gene of interest. A N-terminal 10xHis tag and C-terminal Myc tag is linked to the protein. Following expression, affinity purification is employed to isolate and purify the recombinant Human MATN3 protein from the cell lysate. Denaturing SDS-PAGE is applied to resolve the resulting recombinant Human MATN3 protein, indicating a purity level exceeding 90%.

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Target Background

Function
Major component of the extracellular matrix of cartilage and may play a role in the formation of extracellular filamentous networks.
Gene References into Functions
  1. miR-448 contributed to the progression of osteoarthritis by directly targeting matrilin-3. PMID: 29483929
  2. Study confirmed that MATN3 protein was highly expressed in GAC patients, and MATN3 overexpression could be used as an independent predictor of poor prognosis in GAC patients. PMID: 29343680
  3. our results revealed miR-483-5p directly targeted to the cartilage matrix protein matrilin 3 (Matn3) and tissue inhibitor of metalloproteinase 2 (Timp2) to stimulate chondrocyte hypertrophy, extracellular matrix degradation, and cartilage angiogenesis, and it consequently initiated and accelerated the development of OA. PMID: 28139355
  4. The results of the study indicate a potential role for the MATN3 rs28598872 polymorphism in the pathogenesis of Temporomandibular Joint Internal Derangement. PMID: 27533128
  5. This report is the first to show the involvement of MATN3 in C-type natriuretic peptide/natriuretic peptide receptor-B signaling pathway during the process of transforming growth factor-beta induced chondrogenic differentiation of mesenchymal stem cells. PMID: 24934313
  6. MATN3 plays a regulatory role in cartilage homeostasis due to its capacity to induce IL-1Ra, upregulate gene expression of major cartilage matrix components, and downregulate the expression of OA-associated matrix-degrading proteinases in chondrocytes. PMID: 22967398
  7. MATN3 may have the inherent ability to inhibit premature chondrocyte hypertrophy by suppressing BMP-2/Smad1 activity PMID: 25331953
  8. The VWA1 domain of matrilin-3 is primarily responsible for the induction of IL-6 release from primary human chondrocytes. PMID: 23523902
  9. Polymorphism in the MATN3 gene might play a role in osteoarthritis in the Chinese Han population. PMID: 22973175
  10. Haplotype-4 of MATN3 is associated with vertebral fracture risk independent of bone mineral density in Chinese postmenopausal women. PMID: 22270056
  11. MATN3 mutations were identified in 13 multiple epiphyseal dysplasia patients and comprised predominantly of missense mutations. PMID: 21922596
  12. Radiographic findings in patients with COMP and MATN3 mutations showed marked abnormalities in hip and knee joints. PMID: 21965141
  13. a matrilin-3 mutation associated with osteoarthritis does not affect collagen affinity but promotes the formation of wider cartilage collagen fibrils PMID: 20077500
  14. increased expression of MATN3 in osteoarthritis might contribute to the degeneration of articular cartilage. PMID: 18759284
  15. potential of matrilin-3 to modulate gene expression profile of primary chondrocytes; tested matrilin3-dependent induction of pro-inflammatory cytokines, inducible nitric oxide synthetase & cyclooxygenase-2, MMP1, -3 & -13, & matrilin-3 itself PMID: 19840795
  16. Mutation in MATN3 had significant association for patients with osteoarthritis. PMID: 12736871
  17. Four novel missense mutations and one recurrent missense mutation were identified in MATN3 in seven families with multiple epiphyseal dysplasia. PMID: 14729835
  18. MATN3 mutations is associated with multiple epiphyseal dysplasia PMID: 14994237
  19. Contrary to the previous assumption that the MATN3 mutation in multiple epiphyseal dysplasia is confined to the beta-sheet regions, one novel mutation is located outside the beta-sheet region, within an alpha-helix region PMID: 15459972
  20. COMP, type IX collagen and MATN3 play important roles in matrix assembly PMID: 15694129
  21. mutations in matrilin-3 causing chondrodysplasias (R116W and C299S) interfere with intracellular protein trafficking and formation of filamentous extracellular structures PMID: 16199550
  22. Multiple epiphyseal dysplasia caused by MATN3 mutations is the result of an intracellular retention of the mutant protein. PMID: 16287128
  23. Patients carrying the T(303)M mutation in the gene for matrilin-3 express a form of HOA that is radiologically indistinguishable from idiopathic HOA in individual patients but they have more severe thumb-base involvement, particularly in the STT joint. PMID: 16641049
  24. We have demonstrated intergenic splicing between two sets of family genes, the matrilin-3 (MATN3) and lysosomal-associated protein transmembrane 4alpha (LAPTM4A). PMID: 16769693
  25. recombinant ADAMTS-4 effectively cleaved intact matrilin-3 at the predicted motif at Glu435/Ala436 generating two species of 45 and 5 kDa PMID: 17311924
  26. a matn3 mutation causes decreased chondrocyte proliferation and dysregulated apoptosis leading to epiphyseal dysplasia PMID: 17517694
  27. the matrilin-3 A-domain appears to bind exclusively to the COL3 domain of type IX collagen and this binding is abolished in the presence of a disease causing mutation in type IX collagen PMID: 17881354
  28. The characterization of two additional alpha-helical mutations (p.Ala173Asp and p.Lys231Asn) is described. Both p.Phe105Ser and pAla173Asp prevent the secretion of A-domain in vitro. PMID: 18205203

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Involvement in disease
Multiple epiphyseal dysplasia 5 (EDM5); Spondyloepimetaphyseal dysplasia MATN3-related (SEMD-MATN3); Osteoarthritis 2 (OS2)
Subcellular Location
Secreted.
Tissue Specificity
Expressed only in cartilaginous tissues, such as vertebrae, ribs and shoulders.
Database Links

HGNC: 6909

OMIM: 140600

KEGG: hsa:4148

STRING: 9606.ENSP00000383894

UniGene: Hs.656199

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