Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Target Names

MXD1

Uniprot No.

Q05195

Research Area

Cancer

Alternative Names

Protein MAD

Source

E.coli

Expression Region

1-221aa

Target Protein Sequence

MAAAVRMNIQMLLEAADYLERREREAEHGYASMLPYNNKDRDALKRRNKSKKNNSSSRSTHNEMEKNRRAHLRLCLEKLKGLVPLGPESSRHTTLSLLTKAKLHIKKLEDCDRKAVHQIDQLQREQRHLKRQLEKLGIERIRMDSIGSTVSSERSDSDREEIDVDVESTDYLTGDLDWSSSSVSDSDERGSMQSLGSDEGYSSTSIKRIKLQDSHKACLGL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

54.3 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal EGFP-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Component of a transcriptional repressor complex together with MAX. In complex with MAX binds to the core DNA sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing with MYC for MAX binding. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.

Gene References into Functions

Results suggest a role for MAX dimerization protein 1 (MXD1) in the control of ribosome biogenesis. PMID: 27588501
Study revealed that HIF-1alpha-induced Mxd1 contributes to cisplatin-resistance in hypoxic osteosarcoma cells by directly repressing PTEN, which leads to the activation of PI3K/AKT antiapoptotic and survival pathway. PMID: 28543796
the miR-382-5p/MXD1 axis plays a critical role in myelopoiesis by affecting the lineage choice of CD34(+) hematopoietic stem/progenitor cells. PMID: 27520398
TCP10L stabilizes MAD1 protein level through direct interaction, and they cooperatively regulate cell cycle progression. PMID: 26698869
down regulation of miR-202 increased the expression of its target Mxd1, followed by Mxd1 recruitment to the Sin3A repressor complex and through its dimerization with Max, and increased repression of Myc-Max target proteins. PMID: 25611699
miR-17-92 cluster members miR-19a/b facilitated gastric cancer cell migration, invasion and metastasis through targeting the antagonist of c-Myc -- MXD1. PMID: 24675462
This study suggests that genetic variants of MXD1 may modulate the effect of smoking on carotid plaque burden. PMID: 24954085
Mad1 gene transfer inhibits the proliferation of human melanoma cells PMID: 12366697
HBP1 and Mad1 repressors bind the Sin3 corepressor PAH2 domain with opposite helical orientations PMID: 15235594
MAD1 and Proto-Oncogene Proteins c-myc reciprocally regulate ribosomal DNA transcription, providing a mechanism for coordination of ribosome biogenesis and cell growth PMID: 15282543
c-myc and mad1 can regulate the hTERT transcript in a different manner in hTERT positive cells, but not in normal cells PMID: 15645079
Mad1, Mxi1 and Rox genes were expressed and displayed mutations in haematological malignancies. PMID: 17577784
This study provides a direct link between the growth factor signaling pathways regulated by PI3 kinase/Akt and MAP kinases with Myc-mediated transcription. PMID: 18451027
TGF-beta can override Myc activity despite a stabilizing cancer mutation and induce senescence in myeloid tumor cells, at least in part by induction of Mad1 PMID: 19766114
examination of functionality of basic domains compared with Myc PMID: 12538578
X-ray structures of Myc-Max and Mad-Max recognizing DNA. Molecular bases of regulation by proto-oncogenic transcription factors. PMID: 12553908
Mad expression and Id2 down-regulation are important events during the TGF-beta cytostatic program in epithelial cells. PMID: 12824180
Max as a novel co-activator of C/EBPalpha functions, thereby suggesting a possible link between C/EBPalpha and Myc-Max-Mad network. PMID: 17082780
The PTEN tumor suppressor gene is a target of MAD1. PMID: 17998413
Mxd1 D112a and Max N78a and H81d, which are located in the leucine zippers of the proteins, can dictate the specificity of heterodimerization and whether or not the Mxd1/Max/DNA complex forms. PMID: 18155722
Results suggest STAT3 functions as a C/EBPbeta cofactor in the regulation of the MAD1 gene. PMID: 18203738
Missense mutations in Mad1, Mxi1 and Rox were found in acute leukemia patients. PMID: 18457265
results suggest that phosphorylation of MAD1 by AKT inhibits MAD1-mediated transcription suppression and subsequently activates the transcription of MAD1 target genes PMID: 19526459

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Subcellular Location

Nucleus.

Database Links

HGNC: 6761

OMIM: 600021

KEGG: hsa:4084

STRING: 9606.ENSP00000264444

UniGene: Hs.468908

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Code	CSB-EP015251HU
Abbreviation	Recombinant Human MXD1 protein
MSDS	MSDS Datasheet
Size	$224
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Max dimerization protein 1 (MXD1)

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