Recombinant Human Melanoma-associated antigen D4 (MAGED4)

Code CSB-YP836236HU
MSDS
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Source Yeast
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Code CSB-EP836236HU
MSDS
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Source E.coli
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Code CSB-EP836236HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP836236HU
MSDS
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Source Baculovirus
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Code CSB-MP836236HU
MSDS
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Source Mammalian cell
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Product Details

Purity
≥85% (SDS-PAGE)
Target Names
MAGED4
Uniprot No.
Alternative Names
MAGED4; KIAA1859; MAGED4A; MAGEE1;; MAGED4BMelanoma-associated antigen D4; MAGE-D4 antigen; MAGE-E1 antigen
Species
Homo sapiens (Human)
Expression Region
1-741
Target Protein Sequence
MAEGSFSVQS ESYSVEDMDE GSDEVGEEEM VEGNDYEEFG AFGGYGTLTS FDIHILRAFG SLGPGLRILS NEPWELENPV LAQTLVEALQ LDPETLANET AARAANVARA AASNRAARAA AAAARTAFSQ VVASHRVATP QVSGEDTQPT TYAAEAQGPT PEPPLASPQT SQMLVTSKMA APEAPATSAQ SQTGSPAQEA ATEGPSSACA FSQAPCAREV DANRPSTAFL GQNDVFDFTQ PAGVSGMAFP RPKRPAPAQE AATEGPSAAS GVPQTGPGRE VAATRPKTTK SGKALAKTRW VEPQNVVAAA AAKAKMATSI PEPEGAAAAT AQHSAEPWAR MGGKRTKKSK HLDDEYESSE EERETPAVPP TWRASQPSLT VRAQLAPRPP MAPRSQIPSR HVLCLPPRNV TLLQERANKL VKYLMIKDYK KIPIKRADML KDVIREYDEH FPEIIERATY TLEKKFGIHL KEIDKEEHLY ILVCTRDSSA RLLGKTKDTP RLSLLLVILG VIFMNGNRAS EAVLWEALRK MGLRPGVRHP FLGDLRKLIT DDFVKQKYLE YKKIPNSNPP EYEFLWGLRA RHETSKMRVL RFIAQNQNRD PREWKAHFLE AVDDAFKTMD VDMAEEHARA QMRAQMNIGD EALIGRWSWD DIQVELLTWD EDGDFGDAWA RIPFAFWARY HQYILNSNRA NRRATWRAGV SSGTNGGAST SVLDGPSTSS TIRTRNAARA GASFFSWIQH R
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.
Gene References into Functions
  1. The MAGE-D4 polyclonal antibody with high specificity and sensitivity has been successfully prepared. PMID: 28031124
  2. Preoperative serum MAGED4 was significantly higher in esophageal squamous cell carcinoma patients than in controls. PMID: 25951896
  3. Results suggest that MAGE-D4 overexpression influences tumor progression, and MADE-D4 can be a prognostic marker and a potential molecular target in squamous cell esophageal cancer. PMID: 24147998
  4. These findings suggest MAGE-D4 may serve as a potentially prognostic biomarker and an attractive target of immunotherapy in colorectal cancer PMID: 25120768
  5. MAGE-D4 is highly expressed in glioma and can develop specifically humoral response in glioma patients. PMID: 24966945
  6. MAGED4 (mRNA and protein) is frequently and highly expressed in glioma and is partly regulated by DNA methylation. PMID: 24870746

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Tissue Specificity
Expressed only in brain and ovary among normal tissues. Isoform 1 and isoform 2 are specifically expressed in glioma cells among cancer cells. Detected in some renal cell carcinoma samples.
Database Links

HGNC: 23793

OMIM: 300702

KEGG: hsa:728239

STRING: 9606.ENSP00000364777

UniGene: Hs.522650

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China
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