Recombinant Human Transport and Golgi organization protein 1 homolog (MIA3), partial

Code CSB-YP711448HU
MSDS
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Source Yeast
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Code CSB-EP711448HU
MSDS
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Source E.coli
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Code CSB-EP711448HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP711448HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP711448HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MIA3
Uniprot No.
Alternative Names
ARNT; C219 reactive peptide; C219-reactive peptide; D320; Melanoma inhibitory activity family; member 3; Melanoma inhibitory activity protein 3; Mia3; MIA3_HUMAN; RP11-378J18.4; TANGO; Transport and Golgi organization protein 1; UNQ6077
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum. Required for correct assembly of COPII coat components at endoplasmic reticulum exit sites (ERES) and for the localization of SEC16A and membrane-bound ER-resident complexes consisting of MIA2 and PREB/SEC12 to ERES.
Gene References into Functions
  1. ADTRP positively regulates PIK3R3 expression, which leads to activation of AKT and up-regulation of MIA3/TANGO1, thereby regulating endothelial cell functions directly relevant to atherosclerosis. PMID: 28341552
  2. TANGO1/CTAGE5 receptor serves as a polyvalent template for assembly of large COPII coats. PMID: 27551091
  3. Mammalian endoplasmic reticulum exit sites are organized by TANGO1 acting as a scaffold, in cooperation with Sec16 for efficient secretion. PMID: 28442536
  4. TANGO1 is thus pivotal in concentrating procollagen VII in the lumen and recruiting ERGIC membranes on the cytoplasmic surface of the endoplasmic reticulum. PMID: 26568311
  5. TANGO expression was strongly associated with tumour progression, nodal metastasis, clinical stage and number of blood or lymph vessels in oral squamous cell carcinoma. PMID: 24889917
  6. We conclude that SNP rs17465637 in MIA3 is indeed a genetic risk factor for CAD across different ethnic populations including Han Chinese. PMID: 24125424
  7. results suggest a potential association of the MIA3 rs17465637 with cardiovascular disease in dyslipidemic patients with rheumatoid arthritis PMID: 22577832
  8. genetic polymorphism is associated with an increased risk of myocardial infarction PMID: 21264445
  9. Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator) PMID: 20738937
  10. Observational study of gene-disease association. (HuGE Navigator) PMID: 20971364
  11. Observational study of gene-disease association. (HuGE Navigator) PMID: 20694560
  12. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
  13. TANGO transfected cell lines revealed that TANGO expression reduces motility, whereas reduction of TANGO enhances migration. PMID: 17044017
  14. The results from this study present TANGO as a novel ligand for CD11c, regulating migratory processes of hematopoietic cells. PMID: 17726152
  15. Reduced expression of TANGO is associated with colon and hepatocellular carcinomas PMID: 17786351
  16. Observational study of gene-disease association. (HuGE Navigator) PMID: 19955471
  17. Observational study of gene-disease association. (HuGE Navigator) PMID: 19336575
  18. Observational study and genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 19198609

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Subcellular Location
Endoplasmic reticulum membrane; Single-pass membrane protein.
Protein Families
MIA/OTOR family, Tango1 subfamily
Tissue Specificity
Broadly expressed, except in bone marrow and peripheral blood mononuclear cells. Down-regulated in melanoma tissue.
Database Links

HGNC: 24008

OMIM: 613455

KEGG: hsa:375056

STRING: 9606.ENSP00000340900

UniGene: Hs.118474

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