Recombinant Human Metallothionein-1X(MT1X),partial

In Stock
Code CSB-EP015119HU
Size $1812
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details


The DNA coding sequence translated into the human MT1X protein sequence (1-59aa) was fused with the N-terminal GST tag sequence to form the recombinant DNA, which was inserted into an expression vector. The reconstructed expression vector was transformed into the E.coli for follow-up expression. The product underwent purification to obtain the recombinant human MT1X protein with N-terminal GST tag. The SDS-PAGE analysis determined its purity higher than 90%. After electrophoresis, a 32 kDa protein band was observed on the gel.

MT1X is a gene encoding a protein named Metallothionein-1X (abbreviated MT1X) in human and belongs to Metallothionein superfamily. Metallothionein (MT) is a kind of metal-binding protein rich in cysteine and ubiquitous. It has important significance for maintaining the metabolic balance of some essential metals in the body, resisting toxic metal toxicity, and clearing free radicals. Human MT genes include 10 functional genes: MT1A, MT1B, MT1E, MT1F, MT1G, MT1H, MT1X, MT2A, MT3 and MT4. A study has shown that cells with high expression of MT1X have strong anti-apoptotic properties, which can induce cisplatin resistance in cells.

Purity Greater than 90% as determined by SDS-PAGE.
Target Names MT1X
Uniprot No. P80297
Research Area Others
Alternative Names
MT1X; Metallothionein-1X; MT-1X; Metallothionein-IX; MT-IX
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-59aa
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 32.9kDa
Protein Length Partial
Tag Info N-terminal GST-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 3-7 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids. May be involved in FAM168A anti-apoptotic signaling.
Gene References into Functions
  1. Data show that in the MCF-7 breast cancer cell line, only MT1X metallothioneins (MTs) positively correlated with vascular endothelial growth factor C (VEGFC). PMID: 27107086
  2. MT1XT20 Single Quasi-Monomorphic Mononucleotide Marker showing Microsatellite Instability is associated with Persian Lynch Syndrome PMID: 27797228
  3. These findings suggest that functional interactions between FHL3 and MT-1X may provide some clues to the mechanisms of FHL3-regulated cell proliferation. PMID: 24690879
  4. Results show variation in MTX1 is implicated in disordered gambling PMID: 22780124
  5. siRNA-mediated knockdown of TCRP1 and MT1X was found to sensitize cells to cisplatin, leading to increased apoptosis and inhibition of cell proliferation PMID: 23251525
  6. Our results suggest that MT1XT20 represents a sensitive and specific marker for MSI testing and could be included in a complete set of MSI markers for the confident identification of familial or sporadic dMMR patients in colorectal cancers. PMID: 22108904
  7. It plays a role in the detoxification of heavy metals and reactive oxygen species. (review) PMID: 19881214

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Protein Families Metallothionein superfamily, Type 1 family
Database Links

HGNC: 7405

OMIM: 156359

KEGG: hsa:4501

STRING: 9606.ENSP00000377995

UniGene: Hs.374950

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