Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

MBD2

Uniprot No.

Q9UBB5

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

(Demethylase)(DMTase)(Methyl-CpG-binding protein MBD2)

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

143-411aa

Target Protein Sequence

ATESGKRMDCPALPPGWKKEEVIRKSGLSAGKSDVYYFSPSGKKFRSKPQLARYLGNTVDLSSFDFRTGKMMPSKLQKNKQRLRNDPLNQNKGKPDLNTTLPIRQTASIFKQPVTKVTNHPSNKVKSDPQRMNEQPRQLFWEKRLQGLSASDVTEQIIKTMELPKGLQGVGPGSNDETLLSAVASALHTSSAPITGQVSAAVEKNPAVWLNTSQPLCKAFIVTDEDIRKQEERVQQVRKKLEEALMADILSRAADTEEMDIEMDSGDEA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

45.2 kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-KSI-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Human MBD2 was expressed with the amino acid range of 143-411. The theoretical molecular weight of the MBD2 protein is 45.2 kDa. This protein is generated in a e.coli-based system. The MBD2 coding gene included the N-terminal 6xHis-KSI tag, which simplifies the detection and purification processes of the recombinant MBD2 protein in following stages of expression and purification.

Methyl-CpG-binding domain protein 2 (MBD2) is a key epigenetic regulator that plays a crucial role in interpreting DNA methylation patterns in the genome. As a member of the MBD family, MBD2 specifically recognizes and binds to methylated CpG dinucleotides, influencing chromatin structure and gene expression. MBD2 is involved in gene silencing and transcriptional regulation by recruiting chromatin remodeling complexes and histone deacetylases to methylated DNA regions. It is implicated in various cellular processes, including embryonic development, genomic imprinting, and X-chromosome inactivation. Furthermore, MBD2 has been studied in the context of cancer, where alterations in DNA methylation patterns contribute to aberrant gene expression. Understanding the intricate functions of MBD2 provides insights into epigenetic mechanisms and their impact on cellular processes and disease.

Target Background

Function

Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.

Gene References into Functions

Suggest that reduced MBD2 expression could contribute to chronic airway inflammation in COPD. PMID: 29535511
knockdown of MBD2 by siRNA attenuated vancomycin-induced apoptosis, caspase activity, and the expression of BAX and cleaved caspase 3. PMID: 29022913
CpG and methylation-dependent DNA binding and dynamics of the MBD2 protein at the single-molecule level have been reported. PMID: 28637186
Results show that MBD2 mediates epigenetic silencing of Htra1. PMID: 27388476
MBD2 has a critical role in 'rewriting' the cancer methylome at specific regulatory regions. PMID: 27593931
MBD2 expression was elevated in HCC tissue, which suggesting MBD2 as a candidate prognostic marker of HCC. PMID: 27315121
downregulation of miR-221 inhibits cell migration and invasion at least partially through targeting MBD2 in the human OSCC cell line UM1. PMID: 26788506
Specifically, methyl-CpG-binding domain protein 2 (MBD2) is revealed to be recruited to DNA damage sites after laser microirradiation, which was mediated through MBD domain and MBD2 C-terminus. PMID: 26827827
MBD2 targets short interspersed nuclear elements, but does not exclude RNA Polymerase III. PMID: 25798578
The dynamics of MBD2 deposition across methylated DNA regions was associated with the oncogenic transformation of human mammary cells. PMID: 26007656
Data suggest that MBD2 binds primarily at highly methylated regions, with a strong preference for CpG islands and highlights that MBD2 binding sites display increased methylation in primary breast cancer tissues as compared to normal mammary cells. PMID: 24927503
Biophysical analyses show that the MBD2IDR is an intrinsically disordered region (IDR). However, despite this inherent disorder, MBD2IDR increases the overall binding affinity of MBD2 for methylated DNA. PMID: 25753662
This study investigates the genetic association between methyl-CpG-binding domain (MBD) gene polymorphisms and schizophrenia. PMID: 24849540
alternatively spliced isoforms support pluripotency of stem cells PMID: 24813856
The methylated-DNA binding protein MBD2 enhances NGFI-A (egr-1)-mediated transcriptional activation of the glucocorticoid receptor. PMID: 25135974
These data point to a potential new approach in targeting the DNA methylation machinery by combination of MBD2 and DNMT inhibitors. PMID: 25178277
MBD2 seems to play a selective role in gene repression depending on the CpG content of the promoter region PMID: 23888954
Methylated DNA binding domain protein 2 (MBD2) coordinately silences gene expression through activation of the microRNA hsa-mir-496 promoter in breast cancer cell line. PMID: 24204564
reduced mRNA expression of MBD2 and MBD3 is implicated in gastric carcinogenesis. PMID: 24338710
global DNA hypomethylation is one of the possible epigenetic variations associated with the complex etiology of TOF and significantly correlates with the aberrant expression of MBD2 mRNA in patients with TOF. PMID: 23820632
MBD2 binds a significant fraction of the hypomethylated genes. PMID: 23955541
Abnormal expression levels of DNA (cytosine-5-)-methyltransferase 1 and MBD2 mRNA may be important causes of the global hypomethylation observed in CD4+T cells in systemic lupus erythematosus. PMID: 23127209
In metastatic colorectal cancer cells, reduced levels of miR-221* and miR-224 increase levels of MBD2, thereby decreasing expression of the metastasis suppressor maspin. PMID: 23770133
MBD3 is enriched at active promoters, whereas MBD2 is bound at methylated promoters and enriched at exon sequences of active genes. PMID: 23361464
These factors lead to a binding affinity hierarchy of p66alpha for the different MBD2 homologues (MBD2 approximately MBD3 > MBD3L1 approximately MBD3L2). PMID: 23239876
repressive functions of MBD2-containing NuRD complexes are dependent on cooperative interactions between the major domains of CHD4 with histones and DNA and on binding of methylated DNA by MBD2 PMID: 23071088
MiR-373 behaves as a direct transcriptional target and negative regulator of MBD2 activity through a feedback loop of CpG island methylation in hilar cholangiocarcinoma PMID: 22876037
The association between MBD2 binding and transcriptional repression weakened as the distance between binding site and TSS increased, suggesting that MBD2 represses transcriptional initiation PMID: 22048253
A two-stage association study identifies methyl-CpG-binding domain protein 2 gene polymorphisms as candidates for breast cancer susceptibility. PMID: 22258532
These results show a role for MBD2 in cancer progression and provide support for the prospect of targeting MBD2 therapeutically in aggressive breast cancers. PMID: 21693597
MBD2 overexpression during gliomagenesis may drive tumor growth by suppressing the antiangiogenic activity of a key tumor BAI1. PMID: 21724586
human epsilon-globin gene is subject to multilayered silencing mediated in part by MBD2 PMID: 21296012
In hilar cholangiocarcinoma, down-expression of miR-373 leads to increase of MBD2, which in turn suppresses the methylation-mediated gene such as RASSF1A. PMID: 21086164
This study demonstrated that patients with SLE had a significantly lower level of DNA methylation than the controls, and that expression of both DNMT1 and MBD2 mRNA was significantly increased in the SLE patients compared with controls. PMID: 21078759
Expression level of MBD2 is significantly lower in endometriotic lesions compared with disease-free controls. PMID: 21316665
miR-373 is a methylation-mediated gene and the implication of MBD2 in methylation-mediated suppression of miR-373 plays an important role in tumourigenesis and development in hilar cholangiocarcinoma. PMID: 21165562
The anti-TNFalpha biological agents do not seem to affect DNA methylation and mRNA expressions of DNMT1 and MBD2 in RA PMID: 20937307
MBD2 and ERalpha drive opposite effects on pS2 expression, which are associated with specific steady state levels of histone H3 acetylation and methylation marks PMID: 20300195
Methyl-CpG binding domain protein 2 represses transcription from hypermethylated pi-class glutathione S-transferase gene promoters in hepatocellular carcinoma cells PMID: 11960994
MBD2 protein activates CpG sites within the promoter region of reporter genes PMID: 12177048
interaction with two highly related p66 proteins PMID: 12183469
interacts with latency-associated nuclear antigen of Kaposi's Sarcoma-associated herpesvirus (KSHV)to tether KSHV to cell chromosomes PMID: 12388720
MBDin relieves MBD2 repression potential and reactivates transcription from methylated promoters PMID: 12588985
Results show that methyl-CpG binding domain protein 1 (MBD1) is expressed in tumor cells, but methyl-CpG binding domain protein 2 (MBD2) and methyl CpG binding protein 2 (MeCP2) are not. PMID: 12646234
MBD2a and RNA helicase A cooperatively enhanced CREB-dependent gene expression. PMID: 12665568
MBD2 has a role in the methylation-mediated inhibition of ribosomal RNA gene expression PMID: 14610093
Antisenses oligoDNA suppresses tumor growth in nude mice, a potential anticaner therapy approach. PMID: 14688029
MBD2 gene expression may be significant factor in tumorigenesis. PMID: 15112265
role of MBD3L1 as a methylation-dependent transcriptional repressor that may interchange with MBD3 as an MBD2-interacting component of the NuRD complex PMID: 15456747
MBD3L2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing PMID: 15701600

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Subcellular Location

Nucleus. Note=Nuclear, in discrete foci. Detected at replication foci in late S phase.

Tissue Specificity

Highly expressed in brain, heart, kidney, stomach, testis and placenta.

Database Links

HGNC: 6917

OMIM: 603547

KEGG: hsa:8932

STRING: 9606.ENSP00000256429

UniGene: Hs.25674

Code	CSB-EP890654HU1
Abbreviation	Recombinant Human MBD2 protein, partial
MSDS	MSDS Datasheet
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Methyl-CpG-binding domain protein 2 (MBD2), partial

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