Recombinant Human Mini-chromosome maintenance complex-binding protein (MCMBP)

Code CSB-YP880100HU
MSDS
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Source Yeast
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Code CSB-EP880100HU
MSDS
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Source E.coli
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Code CSB-EP880100HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP880100HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP880100HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MCMBP
Uniprot No.
Alternative Names
MCMBP; C10orf119; Mini-chromosome maintenance complex-binding protein; MCM-BP; MCM-binding protein
Species
Homo sapiens (Human)
Expression Region
1-642
Target Protein Sequence
MPCGEDWLSH PLGIVQGFFA QNGVNPDWEK KVIEYFKEKL KENNAPKWVP SLNEVPLHYL KPNSFVKFRC MIQDMFDPEF YMGVYETVNQ NTKAHVLHFG KYRDVAECGP QQELDLNSPR NTTLERQTFY CVPVPGESTW VKEAYVNANQ ARVSPSTSYT PSRHKRSYED DDDMDLQPNK QKDQHAGARQ AGSVGGLQWC GEPKRLETEA STGQQLNSLN LSSPFDLNFP LPGEKGPACL VKVYEDWDCF KVNDILELYG ILSVDPVLSI LNNDERDASA LLDPMECTDT AEEQRVHSPP ASLVPRIHVI LAQKLQHINP LLPACLNKEE SKTCKFVSSF MSELSPVRAE LLGFLTHALL GDSLAAEYLI LHLISTVYTR RDVLPLGKFT VNLSGCPRNS TFTEHLYRII QHLVPASFRL QMTIENMNHL KFIPHKDYTA NRLVSGLLQL PSNTSLVIDE TLLEQGQLDT PGVHNVTALS NLITWQKVDY DFSYHQMEFP CNINVFITSE GRSLLPADCQ IHLQPQLIPP NMEEYMNSLL SAVLPSVLNK FRIYLTLLRF LEYSISDEIT KAVEDDFVEM RKNDPQSITA DDLHQLLVVA RCLSLSAGQT TLSRERWLRA KQLESLRRTR LQQQKCVNGN EL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Associated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP; probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion.
Gene References into Functions
  1. the MCMBP protein accumulates to high levels in cancer cells, whereas in normal proliferating tissue its abundance is low, indicating that MCMBP could be exploited as a novel diagnostic marker for this type of carcinoma. PMID: 25246271
  2. Data indicate that MCM-BP binds USP7 on chromatin and can mediate an interaction between the USP7 and MCM proteins. PMID: 24190967
  3. results suggest that MCM-BP makes multiple contributions to human cells that are not limited to unloading of the MCM complex PMID: 22250201
  4. MCM-BP can decrease phosphorylation by DDK but is not a substrate. PMID: 22540012
  5. MCM-BP silencing in human cells also delays MCM dissociation in late S phase PMID: 21196493
  6. Knockdown of the human ETG1 results in defective chromatid cohesion. PMID: 20090939
  7. MCM-BP is conserved in multicellular eukaryotes and shares limited homology with MCM proteins. MCM-BP formed a complex with MCM3 to MCM7, which excluded MCM2. PMID: 17296731

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Subcellular Location
Nucleus. Note=Associates with chromatin. Highly associated with chromatin in G1/S and S phases, reduced binding to chromatin in G2, and further decreased binding in early M phase. It then reassociates with chromatin in late M phase. Dissociates from chromatin later than component of the MCM complex.
Protein Families
MCMBP family
Database Links

HGNC: 25782

OMIM: 610909

KEGG: hsa:79892

STRING: 9606.ENSP00000353098

UniGene: Hs.124246

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