Recombinant Human Mitochondrial sodium/hydrogen exchanger 9B2 (SLC9B2), partial

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

SLC9B2

Uniprot No.

Alternative Names

SLC9B2; NHA2; NHEDC2; Sodium/hydrogen exchanger 9B2; Na(+)/H(+) exchanger NHA2; Na(+)/H(+) exchanger-like domain-containing protein 2; NHE domain-containing protein 2; Sodium/hydrogen exchanger-like domain-containing protein 2; Solute carrier family 9 subfamily B member 2

Species

Homo sapiens (Human)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Na(+)/H(+) antiporter that extrudes Na(+) or Li(+) in exchange for external protons across the membrane. Contributes to the regulation of intracellular pH, sodium homeostasis, and cell volume. Plays an important role for insulin secretion and clathrin-mediated endocytosis in beta-cells. Involved in sperm motility and fertility. It is controversial whether SLC9B2 plays a role in osteoclast differentiation or not.

Gene References into Functions

The exchange activity of NHA2 (SLC9B2) is electroneutral, despite harboring the 2 conserved aspartic acid residues found in NapA and other bacterial homologues. The equivalent residue to K305 in human NHA2 has been replaced with arginine, which is a mutation that makes NapA electroneutral. A transmembrane embedded lysine residue is essential for electrogenic transport in Na(+)/H(+) antiporters. PMID: 28154142
the ubiquitous tissue distribution of NHA2 suggests that H(+)-coupled transport is more widespread. The coexistence of Na(+) and H(+)-driven chemiosmotic circuits has implications for salt and pH regulation in the kidney. PMID: 22948142
mutation in AA residues V161 and F357 reduced ability of transfected BW31a cells to remove intracellular Na and grow in NaCl-medium; yeast expressing F357 F437 cannot grow in 0.4M NaCl, suggesting these residues also essential for antiporter activity PMID: 20713131
cluster of essential, highly conserved titratable residues located in an assembly region made of two discontinuous helices of inverted topology, each interrupted by an extended chain PMID: 20053353
NHA-oc/NHA2 displays the expected activities of a bona fide cation-proton antiporter and plays a key role(s) in normal osteoclast differentiation and function PMID: 17988971
Na+/H+ antiporter genes may contribute to sodium-lithium countertransport activity and salt homeostasis in humans[NHA1 and NHA2] PMID: 18000046
restricted to the distal convoluted tubule in the kidney PMID: 18508966

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Subcellular Location

Cell membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Cell projection, cilium, flagellum membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein.

Protein Families

Monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family

Tissue Specificity

Widely expressed. High levels detected in the distal tubules of the kidney nephron. Detected in red blood cells (at protein level).

Database Links

HGNC: 25143

OMIM: 611789

KEGG: hsa:133308

STRING: 9606.ENSP00000354574

UniGene: Hs.546482

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Recombinant Human Mitochondrial sodium/hydrogen exchanger 9B2 (SLC9B2), partial

Product Details

Customer Reviews and Q&A

Target Background

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