Recombinant Human Modulator of apoptosis 1 (MOAP1)

Code CSB-EP014693HU
MSDS
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
MOAP1
Uniprot No.
Research Area
Cell Biology
Alternative Names
MAP 1; MAP-1; MAP1; MOAP1; MOAP1_HUMAN; Modulator of apoptosis 1; Modulator of Apoptosis; Paraneoplastic antigen like 4; Paraneoplastic antigen Ma4; paraneoplastic Ma antigen family member 4; PNMA4
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-351aa
Target Protein Sequence
MTLRLLEDWCRGMDMNPRKALLIAGISQSCSVAEIEEALQAGLAPLGEYRLLGRMFRRDENRKVALVGLTAETSHALVPKEIPGKGGIWRVIFKPPDPDNTFLSRLNEFLAGEGMTVGELSRALGHENGSLDPEQGMIPEMWAPMLAQALEALQPALQCLKYKKLRVFSGRESPEPGEEEFGRWMFHTTQMIKAWQVPDVEKRRRLLESLRGPALDVIRVLKINNPLITVDECLQALEEVFGVTDNPRELQVKYLTTYQKDEEKLSAYVLRLEPLLQKLVQRGAIERDAVNQARLDQVIAGAVHKTIRRELNLPEDGPAPGFLQLLVLIKDYEAAEEEEALLQAILEGNFT
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
66.5kDa
Protein Length
Full Length
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Required for death receptor-dependent apoptosis. When associated with RASSF1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation.
Gene References into Functions
  1. RACK1 associates with MOAP-1 via electrostatic associations similar to those observed between MOAP-1/RASSF1A and MOAP-1/TNF-R1. These events illustrate the complex nature of MOAP-1 regulation and characterizes the important role of the scaffolding protein, RACK1, in influencing MOAP-1 biology. PMID: 29470995
  2. These findings reveal a role for MOAP-1 in Fas signaling in the liver by promoting MTCH2-mediated tBid recruitment to mitochondria. PMID: 27320914
  3. UBR5 downregulates proapoptotic MOAP-1 and suggest that UBR5 can confer cisplatin resistance in ovarian cancer. PMID: 27721409
  4. Paraneoplastic Ma antigen-like 1 is suggested as a novel potential clinically useful prognostic biomarker for patients with pancreatic ductal adenocarcinoma. PMID: 25251443
  5. PNMA2 functions as antagonist of MOAP-1 and PNMA1 through heterodimeric interaction PMID: 27003254
  6. MOAP-1 is a tumor suppressor protein linked to the RASSF1A protein PMID: 26269600
  7. miR-25 promotes cell proliferation and inhibits apoptosis in non-small cell lung cancer cells by negatively regulating MOAP1 expression. PMID: 25998847
  8. PB1-F2 protein of influenza A virus interacts with human MOAP-1 protein. PMID: 23236846
  9. Enforced miR-1228 expression can reduce MOAP1 expression and delay the progression of stress-induced cell apoptosis. PMID: 22434376
  10. RASSF1A and MAP-1 are important components between death receptors and the apoptotic machenery. PMID: 15949439
  11. RASSF1A is a tumor suppressor that activates Bax via MOAP-1 PMID: 16344548
  12. Inhibition of ubiquitin-mediated degradation of MOAP1 by apoptotic stimuli promotes BAX function in mitochondria. PMID: 17535899
  13. The association of RASSF1A and MOAP-1 with death receptors involves an ordered recruitment to receptor complexes to promote cell death and inhibit tumor formation. PMID: 18474619

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Protein Families
PNMA family
Tissue Specificity
Widely expressed, with high levels in heart and brain.
Database Links

HGNC: 16658

OMIM: 609485

KEGG: hsa:64112

STRING: 9606.ENSP00000298894

UniGene: Hs.24719

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