Recombinant Human Mucin-5B (MUC5B), partial

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Code CSB-EP875718HU
MSDS
Size $306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
Uniprot No.
Research Area
Cell Biology
Alternative Names
MUC-5B;Cervical mucin;High molecular weight salivary mucin MG1;Mucin-5 subtype B, tracheobronchial;Sublingual gland mucin
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
4186-4295aa
Target Protein Sequence
ELGQVVECSLDFGLVCRNREQVGKFKMCFNYEIRVFCCNYGHCPSTPATSSTAMPSSTPGTTWILTELTTTATTTASTGSTATPSSTPGTAPPPKVLTSPATTPTATSSK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
18.9 kDa
Protein Length
Partial
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The process for producing recombinant Human MUC5B protein in e.coli cells follows a sequence of steps: creating the expression vector that codes for the Human MUC5B protein (4186-4295aa), introducing it into e.coli cells, cultivating and inducing of positive cells for protein expression, lysing cells, and conducting expression analysis. The resultant recombinant Human MUC5B protein is purified from the cell lysate using affinity purification, with its purity exceeding 85%, as determined by SDS-PAGE.

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Target Background

Function
Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus.
Gene References into Functions
  1. the combination of intracellular packing of the MUC5B mucin and the submucosal gland morphology creates an efficient machine for producing linear mucin bundles. PMID: 29440393
  2. resistin induced MUC5AC and MUC5B expression via activation of different signaling pathways in human airway epithelial cells. PMID: 29604272
  3. Genetic variations in the MUC5B gene can influence dental caries. PMID: 28944591
  4. The expression of MUC5B mRNA increased with increases in NH concentration, and was significantly higher compared to that in untreated cells. H. pylori may affect the expression of MUC5AC, MUC2, MUC5B, and MUC6 in AGS cells via CagA and/or NH, but not E-cadherin. PMID: 29869461
  5. MUC5B was identified as PRP-1 receptor in human chondrosarcoma JJ012 cell line using Ligand-receptor capture technology. PMID: 29138803
  6. The MUC5B promoter polymorphism is strongly associated with interstitial lung abnormalities and specific radiologic subtypes of ILA, with varying degrees of heterogeneity in the underlying populations PMID: 28893869
  7. The associations between MUC5B rs35705950 and short telomere length with extent of fibrosis, histopathological features of usual interstitial pneumonia, and reduced survival in patients with chronic hypersensitivity pneumonitis suggest shared pathobiology with IPF, and might help to stratify risk. PMID: 28648751
  8. High-Mobility Group Box 1 Upregulates MUC5AC and MUC5B Expression in Primary Airway Epithelial Cells PMID: 29286856
  9. IL-33 induced MUC5AC mRNA and MUC5AC protein, and also goblet cell hyperplasia at air liquid interface culture in human nasal epithelial cells. In addition to that, IL-33 induced MUC5B and FOXA3, and reduces FOXJmRNA. PMID: 27776277
  10. MUC5B has physiologic functions in the mucus gel that ensure normal mucus clearance. PMID: 27845589
  11. Down-regulation of MUC5B profoundly alters proliferation, migration and invasion of gastrointestinal cancer cells. PMID: 28972071
  12. Mucins and AQP5 gene expression were significantly higher in patients with OME relative to controls. A 2-fold increase in MUC5B correlated with increased hearing loss (air-bone gap: 7.45 dB [95% CI, 2.65-12.24 dB]; sound field: 6.66 dB [95% CI, 6.63-6.69 dB]), effusion viscosity (2.75 mL/mg; 95% CI, 0.89-4.62 mL/mg), middle ear epithelial thickness (3.5 mum; 95% CI, 1.96-5.13 mum), and neutrophil infiltration (odds rat... PMID: 28594978
  13. This study identified rare and common variants in the MUC5B gene that are associated with type 2 diabetes in Han Chinese. These findings suggest that dysregulated MUC5B expression may be involved in the pathogenesis of type 2 diabetes. PMID: 28346466
  14. a critical regulatory domain that contains the MUC5B promoter variant and has a highly conserved forkhead box protein A2 (FOXA2) binding motif, is identified. PMID: 28272906
  15. There is no evidence of major proteolytic processing of D-domains during the production of the mature secreted polymeric mucin in normal and cystic fibrosis primary bronchial epithelial cells. PMID: 26993521
  16. Different rs35705950 SNP alleles are associated with different CT imaging phenotypes of pulmonary fibrosis. PMID: 26836909
  17. MUC5B was significantly more often detected in middle ear effusion fluid relative to MUC5AC. MUC5B presence was statistically associated with mucoid effusions relative to serous effusions. PMID: 27729120
  18. Results collectively indicate unique links between dual-specificity phosphatase 28 (DUSP28) and mucins MUC5B/MUC16 and their roles in pancreatic cancer. PMID: 27230679
  19. Study provides evidence showing that MUC5B expression in cancer cells contributes to certain tumorigenic properties of breast cancer cells, such as cell growth, adhesion, clonogenic ability and drug chemo-resistance. PMID: 26984395
  20. this study shows that histamine activated the NF-kappaB pathway, contributing to MUC5B overproduction and secretion in nasal epithelial cells PMID: 26574733
  21. This study showed that MUC5B minor allele predisposes to poradic idiopathic pulmonary fi brosis (spIPF), familial interstitial pneumonia (FIP) and idiopathic non-speci fi c interstitial pneumonia. In spIPF, survival is not in fl uenced by MUC5B alleles. In FIP, MUC5B minor allele predicts better survival. PMID: 26699835
  22. MUC5B may play a role in the development of pediatric fibrotic lung disease in patients with Surfactant Protein C mutations PMID: 25858779
  23. the MUC5B polymorphism rs35705950 is associated with increased risk of idiopathic pulmonary fibrosis susceptibility, severity, and the decreased overall survival. PMID: 26823827
  24. The MUC5B promoter polymorphism is the strongest and the most replicated genetic risk factor for Idiopathic pulmonary fibrosis. It is involved in disease pathogenesis through an increase in MUC5B expression in terminal bronchi and honeycombed cysts. PMID: 26595739
  25. We propose a mechanism whereby MUC5B decreases surface tension lowering capacity of alveolar surfactant at areas with maximal mechanical stress PMID: 26539479
  26. These results suggest that MUC5B production can be regulated by ECM components and that MUC5B is upregulated by fibronectin and laminin via the integrin, ERK, and NF-kappaB dependent pathway. PMID: 26057585
  27. MUC5B promoter genotype was not associated with high attenuation areas on lung computed tomography. PMID: 26514822
  28. Overexpression of MUC5B has been described in idiopathic pulmonary fibrosis lungs. Read More: http://www.atsjournals.org/doi/full/10.1164/rccm.201507-1322LE#.V2WAGNLrtNs PMID: 26871672
  29. The variant allele of a common MUC5B promoter variant, rs35705950, is significantly associated with both familial and sporadic idiopathic pulmonary fibrosis. Read More: http://www.atsjournals.org/doi/full/10.1164/rccm.201509-1872LE#.V2WBpNLrtNs PMID: 26871673
  30. Mucin 5B promoter polymorphism is associated with the risk for interstitial lung diseases mainly in older male Chinese subjects PMID: 25121989
  31. MUC5B is a novel prognostic biomarker for patients with non-small cell lung cancer (NSCLC)carrying EGFR mutations but not for patients with NSCLC carrying wild-type EGFR PMID: 26224019
  32. MUC5B has recently been shown to be associated with idiopathic pulmonary fibrosis susceptibility and survival. Read More: http://www.atsjournals.org/doi/full/10.1164/rccm.201505-1010OC#.VwqiYdLrvyA PMID: 26331942
  33. strong association between the MUC5B promoter rs35705950 minor T allele and idiopathic pulmonary fibrosis susceptibility particularly evident in the Caucasian population, milder but still significant in the Asian population [meta-analysis] PMID: 26512610
  34. this is the first study to successfully validate the association between rs35705950 and IPF in a Japanese ethnicity. PMID: 25581455
  35. Increased expression of MUC5B was associated with bacterial biofilm formation in chronic rhinosinusitis patients. PMID: 25638393
  36. MUC5B polymorphism confers susceptibility to idiopathic pulmonary fibrosis in Europeans and Asians--{review} PMID: 25926289
  37. Both compounds down regulated mucin 5 subtype B, and peptidoglycan recognition protein 1 in vaginal tissue PMID: 25333937
  38. the combination of MUC5B and TTF-1 expression is useful for discriminating adenocarcinomas from squamous cell carcinomas, yielding prognostic significance in patients with lung adenocarcinoma. PMID: 25733373
  39. An increase of MUC5B abundance found in the sinus secretions of pediatric patients with chronic rhinosinusitis. PMID: 25420179
  40. The T allele at rs35705950 of the MUC5B gene is associated with usual interstitial pneumonitis PMID: 25317858
  41. The expression of a subset of mucins (MUC2, MUC6, MUC5B) was also correlated with sialyl-Tn expression in LS174T cells. PMID: 24840470
  42. These results show for the first time that Staphylococcus enterotoxin A induces MUC5B expression via TLR2, ERK1/2, and p38 MAPK signaling pathway in human airway epithelial cells. PMID: 24717875
  43. The MUC5B rs2672794 CC genotype was associated with a significantly increased risk of coal workers' pneumoconiosis, compared with the TT genotype. PMID: 24924948
  44. Adolescents with very high intensity of dental caries disease had increased levels of MUC1 and MUC5B. PMID: 24441930
  45. Sputum is not inert and degradation reduces apparent mucin concentrations and sputum elasticity. PMID: 24332705
  46. These results suggest that visfatin induces MUC8 and MUC5B expression through p38 MAPK/ROS/NF-kappaB signaling pathway in human airway epithelial cells. PMID: 24885580
  47. TSLP induces MUC5B expression via the ERK1/2 and p38 MAPK signaling pathway in human airway epithelial cells. PMID: 24792379
  48. we report the expression pattern of MUC2, MUC5AC, MUC5B, and MUC6 in a large series of colorectal carcinomas PMID: 23807779
  49. This study suggests that although both MUC5B and TERT polymorphisms confer independent risks for interstitial lung disease (ILD), MUC5B rs35705950 may, in particular, contribute differentially to idiopathic pulmonary fibrosis and other ILD entities. PMID: 24434656
  50. One SNP in the MUC5B gene having association with chronic otitis media with effusion in study population. PMID: 23929584

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Involvement in disease
Pulmonary fibrosis, idiopathic (IPF)
Subcellular Location
Secreted.
Tissue Specificity
Expressed on surface airway epithelia. Expressed mainly in mucous cells of submucosal glands of airway tissues. Highly expressed in the sublingual gland. Also found in submaxillary glands, endocervix, gall bladder, and pancreas.
Database Links

HGNC: 7516

OMIM: 178500

KEGG: hsa:727897

STRING: 9606.ENSP00000436812

UniGene: Hs.523395

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