Recombinant Human MyoD family inhibitor (MDFI)

Code CSB-YP013617HU
MSDS
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Source Yeast
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Code CSB-EP013617HU
MSDS
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Source E.coli
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Code CSB-EP013617HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013617HU
MSDS
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Source Baculovirus
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Code CSB-MP013617HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MDFI
Uniprot No.
Alternative Names
MDFI; MyoD family inhibitor; Myogenic repressor I-mf
Species
Homo sapiens (Human)
Expression Region
1-246
Target Protein Sequence
MYQVSGQRPS GCDAPYGAPS AAPGPAQTLS LLPGLEVVTG STHPAEAAPE EGSLEEAATP MPQGNGPGIP QGLDSTDLDV PTEAVTCQPQ GNPLGCTPLL PNDSGHPSEL GGTRRAGNGA LGGPKAHRKL QTHPSLASQG SKKSKSSSKS TTSQIPLQAQ EDCCVHCILS CLFCEFLTLC NIVLDCATCG SCSSEDSCLC CCCCGSGECA DCDLPCDLDC GILDACCESA DCLEICMECC GLCFSS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways.
Gene References into Functions
  1. MDFI was significantly hypermethylated in colorectal cancer tissues when compared with adjacent normal colorectal tissues. PMID: 28782576
  2. I-mfa domain proteins may be involved in their oncogenic functions by negatively regulating their transcriptional activities. PMID: 21664411
  3. I-mfa functions as a molecular switch to suppress the store dependence of TRPC1 PMID: 14530267
  4. These results suggest that the physical and functional interaction between Zic and I-mfa proteins can play a role in the vertebrate development. PMID: 15207726
Subcellular Location
Nucleus. Cytoplasm.
Protein Families
MDFI family
Database Links

HGNC: 6967

OMIM: 604971

KEGG: hsa:4188

STRING: 9606.ENSP00000230321

UniGene: Hs.520119

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