Product Details

Purity

>85% (SDS-PAGE)

Target Names

MYOF

Uniprot No.

Q9NZM1

Alternative Names

Fer 1 like 3, myoferlin (C. elegans) ; Fer 1 like family member 3; Fer 1 like protein 3; Fer-1-like protein 3; FER1L3; myoF; MYOF_HUMAN; Myoferlin

Species

Homo sapiens (Human)

Source

Yeast

Protein Length

Extracellular domain

Tag Info

N-terminal His-tagged
Tag-Free

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR.

Gene References into Functions

Myoferlin is a general component of cancer cell derived exosomes from different breast and pancreatic cancer cell lines. PMID: 27845903
the cleavage of myoferlin, yielding a membrane-associated dual C2 domain 'mini-myoferlin', is reported. PMID: 28192161
A novel regulator, myoferlin, of ADAM12 is discovered in HeLa cells and this protein increases ADAM12 expression level, stability, and its enzymatic activity, leading to the reduction of its substrate, E-cadherin, which plays important roles in the regulation of cell adhesion and tumor metastasis. PMID: 27432471
Data show myoferlin depletion did not affect STAT3 transcription factor (STAT3) phosphorylation, but completely blocked STAT3 translocation to nucleus. PMID: 28745314
This study is the first to report robust age associations for DNA methylation in MYOF and DDO, both of which have plausible functional roles in aging PMID: 28255110
Results demonstrate for the first time that nuclear myoferlin expression independently predicts poor clinical outcome in OPSCC patients. PMID: 26919244
Myoferlin plays a key role in VEGFA secretion and impacts tumor-associated angiogenesis in human pancreas cancer. PMID: 26311411
MYOF regulates cell adhesions and cell-substrate adhesion strength and may account for the high degree of motility in invasive breast cancer cells. PMID: 25631868
These data provide the first evidence of myoferlin expression in solid human and mouse tumors. PMID: 23499551
Data indicate that dysferlin, otoferlin, and myoferlin do not merely passively adsorb to membranes but actively sculpt lipid bilayers. PMID: 23859474
High myoferlin expression is associated with breast cancer. PMID: 23864327
The effect of myoferlin on the expression of ZO-1 in airway epithelial cells indicates its role in membrane fusion events that regulate cell detachment and apoptosis within the airway epithelium. PMID: 22808170
suggest a novel role of MYOF in breast tumor cell invasion and a potential reversion to an epithelial phenotype upon loss of MYOF PMID: 22761893
MYOF plays a previously unrecognized role in cancer cell invasion. PMID: 22135466
myoferlin forms a complex with dynamin-2 and VEGFR-2, which prevents CBL-dependent VEGFR-2 polyubiquitination and proteasomal degradation. PMID: 17702744
solution structure of the inner DysF domain of the dysferlin paralogue myoferlin, which has a unique fold held together by stacking of arginine and tryptophans, mutations that lead to clinical disease in dysferlin PMID: 18495154
interaction of myoferlin with EHD2 identifies molecular overlap between the endocytic recycling pathway and the machinery that regulates myoblast membrane fusion PMID: 18502764
In trophoblastic cells, there was a positive correlation between cell fusion and increased dysferlin expression but not myoferlin expression. PMID: 19228595
These data describe a new role for myoferlin in receptor-dependent endocytosis and an overlapping role for myoferlin-dynamin 2-caveolin 1 protein complexes in membrane fusion and fission events. PMID: 19494235

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Subcellular Location

Cell membrane; Single-pass type II membrane protein. Nucleus membrane; Single-pass type II membrane protein. Cytoplasmic vesicle membrane; Single-pass type II membrane protein.

Protein Families

Ferlin family

Tissue Specificity

Expressed in myoblast and endothelial cells (at protein level). Highly expressed in cardiac and skeletal muscles. Also present in lung, and at very low levels in kidney, placenta and brain.

Database Links

HGNC: 3656

OMIM: 604603

KEGG: hsa:26509

STRING: 9606.ENSP00000352208

UniGene: Hs.602086