Recombinant Human Myopalladin (MYPN), partial

Code CSB-YP015378HU
MSDS
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Source Yeast
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Code CSB-EP015378HU
MSDS
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Source E.coli
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Code CSB-EP015378HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015378HU
MSDS
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Source Baculovirus
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Code CSB-MP015378HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MYPN
Uniprot No.
Alternative Names
145 kDa sarcomeric protein; MYOP; Myopalladin; MYPN; MYPN_HUMAN; sarcomeric protein myopalladin
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of the sarcomere that tethers together nebulin (skeletal muscle) and nebulette (cardiac muscle) to alpha-actinin, at the Z lines.
Gene References into Functions
  1. Targeted sequencing revealed trigenic mutations: c.700G>A/p.E234K in DES, c.2966G>A/p.R989H in MYPN, and c.5918G>C/p.R1973P in CACNA1C in a family of hypertrophic cardiomyopathy with early repolarization and short QT syndrome. PMID: 28427417
  2. Homozygous truncating mutations in MYPN in 2 unrelated families with a slowly progressive congenital cap myopathy. PMID: 28220527
  3. results suggest that MYPN screening should be considered in individuals with mild nemaline myopathy, especially when cardiac problems or intranuclear rods are present PMID: 28017374
  4. Heterozygote Mypn(WT/Q526X) knock-in mice develop RCM due to persistence of mutant Mypn(Q526X) protein in the nucleus. PMID: 25541130
  5. the clinical significance of myopalladin for the functional integrity of the sarcomeric apparatus and the protection against dilated cardiomyopathy PMID: 22892539
  6. Two nonsense and 13 missense MYPN variants were identified in subjects with hypertrophic, dilated and/or restrictive cardiomyopathy. PMID: 22286171
  7. mutations in PDLIM3 and MYPN are infrequent in hypertrophic cardiomyopathies PMID: 20801532
  8. myopalladin plays a signaling role in targeting and orienting nebulin during sarcomere assembly PMID: 12482578
  9. myopalladin gene is a new gene associated with dilated cardiomyopathy and observed mutations in 3-4% of cases in a population. of European descent. PMID: 18006477

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Involvement in disease
Nemaline myopathy 11 (NEM11); Cardiomyopathy, dilated 1KK (CMD1KK); Cardiomyopathy, familial hypertrophic 22 (CMH22); Cardiomyopathy, familial restrictive 4 (RCM4)
Subcellular Location
Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere. Cytoplasm, myofibril, sarcomere, Z line.
Protein Families
Myotilin/palladin family
Tissue Specificity
Expressed in adult skeletal muscle and fetal heart.
Database Links

HGNC: 23246

OMIM: 608517

KEGG: hsa:84665

STRING: 9606.ENSP00000351790

UniGene: Hs.55205

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