Recombinant Human N-alpha-acetyltransferase 25, NatB auxiliary subunit (NAA25), partial

Code CSB-YP617948HU
MSDS
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Source Yeast
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Code CSB-EP617948HU
MSDS
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Source E.coli
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Code CSB-EP617948HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP617948HU
MSDS
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Source Baculovirus
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Code CSB-MP617948HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NAA25
Uniprot No.
Alternative Names
C12orf30; DKFZp667K2112; FLJ1308; MDM20; Mitochondrial distribution and morphology 20; Mitochondrial distribution and morphology protein 20; N(alpha)-acetyltransferase 25, NatB auxiliary subunit; N-alpha-acetyltransferase 25, NatB auxiliary subunit; N-terminal acetyltransferase B complex subunit MDM20; N-terminal acetyltransferase B complex subunit NAA25; Naa25; NAA25_HUMAN; NAP1; NatB complex subunit MDM20; p120; TPR repeat-containing protein C12orf30
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Non-catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp-Glu. May play a role in normal cell-cycle progression.
Gene References into Functions
  1. Mdm20 acts as a novel regulator of Rictor, thereby controlling mTORC2 activity, and leading to the activation of PKCalphaS657 and FoxO1. PMID: 26600389
  2. The human NatB complex composed of Naa20 (NAT3) and Naa25 (MDM20) N-terminally acetylates Met-Glu-, Met-Asp-, Met-Asn- and Met-Gln- N-termini, and is important for the structure and function of actomyosin fibers and for proper cellular migration. PMID: 22814378
  3. The C120rf30 gene maps into a region of extensive linkage disequilibrium including several genes that represent functional candidates of type 1 diabetes-susceptibility. PMID: 20089178
  4. Human N-acetyltransferase complexe NatB consists of human N-acetyltransferase 3 and human MDM20. The hMDM20 knockdown decreased the fraction of cells in G(0)/G(1)-phase and increased the fraction of cells in the sub-G(0)/G(1)-phase. PMID: 18570629
  5. There are associations between juvenile idiopathic arthritis and variants in the TNFAIP3, STAT4, and C12orf30 regions that have previously shown associations with other autoimmune diseases, including rheumatoid arthritis and systemic lupus erythematosus. PMID: 19565500
  6. hMdm20 (C12orf30) is an auxiliary subunit of the human NatB N-terminal acetyltransferase complex while hNat3 (NAT5) is the catalytic subunit. This ribosome associated complex acetylates nascent Met-Asp/Glu- polypeptides. PMID: 18570629

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Subcellular Location
Cytoplasm.
Protein Families
MDM20/NAA25 family
Database Links

HGNC: 25783

OMIM: 612755

KEGG: hsa:80018

STRING: 9606.ENSP00000261745

UniGene: Hs.530941

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