Recombinant Human NF-kappa-B-activating protein (NKAP)

Code CSB-YP847637HU
MSDS
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Source Yeast
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Code CSB-EP847637HU
MSDS
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Source E.coli
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Code CSB-EP847637HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP847637HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP847637HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NKAP
Uniprot No.
Alternative Names
NF-kappa-B-activating protein; NFKB activating protein; Nkap; NKAP_HUMAN; Nuclear NF kappaB activating protein
Species
Homo sapiens (Human)
Expression Region
1-415
Target Protein Sequence
MAPVSGSRSP DREASGSGGR RRSSSKSPKP SKSARSPRGR RSRSHSCSRS GDRNGLTHQL GGLSQGSRNQ SYRSRSRSRS RERPSAPRGI PFASASSSVY YGSYSRPYGS DKPWPSLLDK EREESLRQKR LSERERIGEL GAPEVWGLSP KNPEPDSDEH TPVEDEEPKK STTSASTSEE EKKKKSSRSK ERSKKRRKKK SSKRKHKKYS EDSDSDSDSE TDSSDEDNKR RAKKAKKKEK KKKHRSKKYK KKRSKKSRKE SSDSSSKESQ EEFLENPWKD RTKAEEPSDL IGPEAPKTLT SQDDKPLNYG HALLPGEGAA MAEYVKAGKR IPRRGEIGLT SEEIASFECS GYVMSGSRHR RMEAVRLRKE NQIYSADEKR ALASFNQEER RKRENKILAS FREMVYRKTK GKDDK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Acts as a transcriptional repressor. Plays a role as a transcriptional corepressor of the Notch-mediated signaling required for T-cell development. Also involved in the TNF and IL-1 induced NF-kappa-B activation. Associates with chromatin at the Notch-regulated SKP2 promoter.
Gene References into Functions
  1. SUMOylated NKAP is essential for chromosome alignment by anchoring CENP-E to kinetochores PMID: 27694884
  2. NKAP is required for T cell maturation and the acquisition of functional competency PMID: 21624937
  3. NKAP is a novel nuclear regulator of TNF- and IL-1-induced NF-kappaB activation PMID: 14550261
  4. NKAP functions as a transcriptional repressor, acting on Notch target genes, and is required for alphabeta T cell development. PMID: 19409814
Subcellular Location
Nucleus.
Protein Families
NKAP family
Database Links

HGNC: 29873

OMIM: 300766

KEGG: hsa:79576

STRING: 9606.ENSP00000360464

UniGene: Hs.522771

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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