Recombinant Human Nanos homolog 3 (NANOS3)

Code CSB-YP015438HU
MSDS
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Source Yeast
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Code CSB-EP015438HU
MSDS
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Source E.coli
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Code CSB-EP015438HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015438HU
MSDS
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Source Baculovirus
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Code CSB-MP015438HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NANOS3
Uniprot No.
Alternative Names
NANO3_HUMAN; Nanos homolog 3; NANOS3; NOS-3; Nos3
Species
Homo sapiens (Human)
Expression Region
1-173
Target Protein Sequence
MGTFDLWTDY LGLAHLVRAL SGKEGPETRL SPQPEPEPML EPDQKRSLES SPAPERLCSF CKHNGESRAI YQSHVLKDEA GRVLCPILRD YVCPQCGATR ERAHTRRFCP LTGQGYTSVY SHTTRNSAGK KLVRPDKAKT QDTGHRRGGG GGAGFRGAGK SEPSPSCSPS MST
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a role in the maintenance of the undifferentiated state of germ cells regulating the spermatogonia cell cycle and inducing a prolonged transit in G1 phase. Affects cell proliferation probably by repressing translation of specific mRNAs. Maintains the germ cell lineage by suppressing both Bax-dependent and -independent apoptotic pathways. Essential in the early stage embryo to protect the migrating primordial germ cells (PGCs) from apoptosis.
Gene References into Functions
  1. despite the prominent role of NANOS3 in ovarian development, our findings suggest that NANOS3 mutations were not associated with POF in the present cohort of Brazilian women. PMID: 28076512
  2. data can be used to further elucidate the role of NANOS3 and DAZL in germ cell development both in vitro and in vivo PMID: 27768780
  3. NANOS3 has role in the acquisition of invasiveness by human lung tumour cells and propose a new mechanism of post-transcriptional regulation of epithelial-mesenchymal transition. PMID: 25904364
  4. an inactivating missense mutation in NANOS3 has a role in primary ovarian insufficiency that involves increased primordial germ cell apoptosis during embryonic cell migration PMID: 25054146
  5. NANOS3 mutation is one possible cause of primary ovarian insufficiency. PMID: 24091668
  6. NANOS3 modulates essential aspects of human germ cell development perhaps during the cell cycle. PMID: 21421998
  7. Mutations in NANOS3 exons are rare in both Chinese and Caucasian women with premature ovarian failure. PMID: 17418157
  8. Report for the first time analysis of the NANOS3 gene in infertile males. PMID: 19565640
  9. Observational study of genotype prevalence. (HuGE Navigator) PMID: 17418157

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Subcellular Location
Nucleus. Cytoplasm. Cytoplasm, Stress granule. Cytoplasm, P-body.
Protein Families
Nanos family
Tissue Specificity
Ovary, testis and brain (at protein level). In the ovaries, expressed during multiple stages of oogenesis, including primordial, primary, secondary and antral follicles with the highest expression in the oocytes. In the testis, expressed in germ cells, ty
Database Links

HGNC: 22048

OMIM: 608229

KEGG: hsa:342977

UniGene: Hs.127982

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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