Recombinant Human Nardilysin (NRD1), partial

Code CSB-YP016075HU
MSDS
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Source Yeast
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Code CSB-EP016075HU
MSDS
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Source E.coli
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Code CSB-EP016075HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP016075HU
MSDS
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Source Baculovirus
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Code CSB-MP016075HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NRDC
Uniprot No.
Alternative Names
NRDC; NRD1; Nardilysin; EC 3.4.24.61; N-arginine dibasic convertase; NRD convertase; NRD-C; Nardilysin convertase
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Cleaves peptide substrates on the N-terminus of arginine residues in dibasic pairs.
Gene References into Functions
  1. NRDC expression was reduced in infarcted regions in autopsy samples from acute myocardial ischemia patients. PMID: 28747015
  2. Gene expression level of NRD1 is significantly higher in AD patients when compared to normal controls. PMID: 26943237
  3. MRNA expression of NRD1 was upregulated in 56% of ESCC tissue samples. PMID: 24168165
  4. This study demonistrated that alcohol-dependent reduction of nardilysin in cell culture and nervous tissue points to an implication of the enzyme in the pathophysiology of alcoholism. PMID: 23219461
  5. possible roles of nardilysin in Alzheimer disease, Down syndrome, schizophrenia, mood disorders, alcohol abuse, heroin addiction and cancer; show that nardilysin is a Janus-faced enzyme with regard to brain pathology-- probably neuropathogenic in some diseases, but neuroprotective in others [review] PMID: 23604405
  6. SH-SY5Y cells, stably transfected with green fluorescent protein-tagged-p42(IP4) show enhanced NRD protein expression already at an earlier time point after retinoic acid stimulation. PMID: 21801775
  7. NRD1 interacts with p53 mutant R273H PMID: 22653443
  8. These results demonstrate that gastric cancer cell growth is maintained by autonomous TNF-alpha-NF-kappaB and IL-6-STAT3 signalling, and that NRDc and ADAM proteases turn on these signalling cascades by stimulating ectodomain shedding of TNF-alpha. PMID: 22351606
  9. Identification and characterization of nardilysin as a novel dimethyl H3K4-binding protein involved in transcriptional regulation. PMID: 22294699
  10. Several flanking SNPs of the top hits in the meta-analysis demonstrated borderline associations with alcohol dependence in the family sample for KIAA0040, NRD1 and THSD7B, respectively. PMID: 21703634
  11. Tubulin potentiates the interaction of the metalloendopeptidase nardilysin with the neuronal scaffold protein p42IP4/centaurin-alpha1 (ADAP1). PMID: 21972134
  12. mediates antigen processing that generates cytotoxic T cell epitopes PMID: 21151101
  13. nardilysin (NRDc) is potently inhibited by heparin-binding epidermal growth factor-like growth factor (HB-EGF) PMID: 12095415
  14. Nardilysin has an essential role in HB-EGF ectodomain shedding, which is regulated by the modulation of sheddase activity PMID: 16923819
  15. We found high staining intensity in the hypothalamus, neocortex and brain stem nuclei. The cellular localization is almost exclusively confined to neurons. In pre- and perinatal human brain cortex, most neurons express the enzyme. PMID: 17442499
  16. These results indicate the involvement of NRDc in ectodomain shedding of TNF-alpha. PMID: 18355445
  17. Nardilysin convertase regulates the function of the maxi-K channel isoform mK44 in human myometrium. PMID: 19118164
  18. N-arginine dibasic convertase is a specific receptor for heparin-binding EGF-like growth factor (HB-EGF) that modulates HB-EGF-induced cell migration. PMID: 11432822
  19. The acidic stretch of nardilysin, expressed as a fusion protein with glutathione S-transferase and compared to the native enzyme with respect to spermine binding, functions as an autonomous domain. PMID: 11478915

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Protein Families
Peptidase M16 family
Tissue Specificity
Primarily in adult heart, skeletal muscle, and testis and at much lower levels in other tissues.
Database Links

HGNC: 7995

OMIM: 602651

KEGG: hsa:4898

STRING: 9606.ENSP00000346890

UniGene: Hs.584782

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