Recombinant Human Neurofilament heavy polypeptide(NEFH),partial

Code CSB-EP015686HU
Product Type Recombinant Protein
Size US$1726
Uniprot No. P12036
Relevance Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP015686HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) NEFH.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP015686HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) NEFH.
Storage Buffer Tris-based buffer,50% glycerol
Alias 200 kDa neurofilament protein Neurofilament triplet H protein
Species Homo sapiens (Human)
Purity Greater than 85% as determined by SDS-PAGE.
Sequence MMSFGGADALLGAPFAPLHGGGSLHYALARKGGAGGTRSAAGSSSGFHSWTRTSVSSVSASPSRFRGAGAASSTDSLDTLSNGPEGCMVAVATSRSEKEQLQALNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRSAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAAARALARFAQEAEAARVDLQKKAQALQEECGYLRRHHQEEVGELLGQIQGSGAAQAQMQAETRDALKCDVTSALREIRAQLEGHAVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRI
Research Area Neuroscience
Source E.coli
Gene Names NEFH
Protein Names Recommended name: Neurofilament heavy polypeptide Short name= NF-H Alternative name(s): 200 kDa neurofilament protein Neurofilament triplet H protein
Expression Region 1-413aa
Tag Info N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Mol. Weight 65.6kDa
Protein Description Partial
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Function Neurofilaments usually contain three intermediate filament proteins
Involvement in disease Amyotrophic lateral sclerosis (ALS); Charcot-Marie-Tooth disease 2CC (CMT2CC)
Subcellular Location Cytoplasm
Protein Families Intermediate filament family
Database Links

HGNC: 7737

OMIM: 105400

KEGG: hsa:4744

STRING: 9606.ENSP00000311997

UniGene: Hs.198760

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