Recombinant Human Neuroplastin (NPTN), partial

Code CSB-YP016028HU
MSDS
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Source Yeast
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Code CSB-EP016028HU
MSDS
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Source E.coli
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Code CSB-EP016028HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP016028HU
MSDS
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Source Baculovirus
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Code CSB-MP016028HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NPTN
Uniprot No.
Alternative Names
GP55; GP65; Neuroplastin; np55; np65; Nptn; NPTN_HUMAN; SDFR1; SDR-1; SDR1; stromal cell derived factor receptor 1; Stromal cell-derived receptor 1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probable homophilic and heterophilic cell adhesion molecule involved in long term potentiation at hippocampal excitatory synapses through activation of p38MAPK. May also regulate neurite outgrowth by activating the FGFR1 signaling pathway. May play a role in synaptic plasticity. Also acts as a chaperone for ATP2B1; stabilizes ATP2B1 and increases its ATPase activity. Promotes localization of XKR8 at the cell membrane.
Gene References into Functions
  1. Cryoelectron microscopy structure offers important insight into how the NPTN binds to the PMCA1. PMID: 30190470
  2. Xkr8-BSG/NPTN complex is required for phosphatidylserine scrambling in apoptotic cell membranes. PMID: 27503893
  3. This study showed that Single nucleotide polymorphism in the neuroplastin locus associates with cortical thickness and intellectual ability in adolescents. PMID: 24514566
  4. correct synaptic membrane protein localization, neuronal energy supply, expression of LTP and LTD, animal and human behaviour, and pathophysiology and disease--{review} PMID: 25040546
  5. NPTN may be involved in genetic susceptibility to schizophrenia. PMID: 17123723
  6. In human brain np65 is prominently present in cerebellum while np55 is the predominant isoform in mouse and rat cerebellum. PMID: 17196182

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cell junction, synapse, postsynaptic density.
Tissue Specificity
Isoform 1 is ubiquitously expressed. Isoform 2 is expressed in brain cortex and cerebellum (at protein level).
Database Links

HGNC: 17867

OMIM: 612820

KEGG: hsa:27020

STRING: 9606.ENSP00000290401

UniGene: Hs.744867

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