Recombinant Human Nitrogen permease regulator 2-like protein(NPRL2)

Code CSB-YP840976HU
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Source Yeast
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Code CSB-EP840976HU
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Source E.coli
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Code CSB-EP840976HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP840976HU
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Source Baculovirus
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Code CSB-MP840976HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names NPRL2
Uniprot No. Q8WTW4
Alternative Names 2810446G01Rik; G21 protein; Gene 21 protein; Homologous to yeast nitrogen permease (candidate tumor suppressor); Homologous to yeast nitrogen permease; Nitrogen permease regulator 2-like protein; Nitrogen permease regulator-like 2 (S. cerevisiae); NPR 2L; NPR L2; NPR like 2; NPR2; NPR2 like; NPR2-like protein; NPR2L; NPRL 2; NPRL2; NPRL2_HUMAN; Tumor suppressor candidate 4; TUSC 4; TUSC 4 protein; TUSC4; TUSC4 protein
Species Homo sapiens (Human)
Expression Region 1-380
Target Protein Sequence MGSGCRIECI FFSEFHPTLG PKITYQVPED FISRELFDTV QVYIITKPEL QNKLITVTAM EKKLIGCPVC IEHKKYSRNA LLFNLGFVCD AQAKTCALEP IVKKLAGYLT TLELESSFVS MEESKQKLVP IMTILLEELN ASGRCTLPID ESNTIHLKVI EQRPDPPVAQ EYDVPVFTKD KEDFFNSQWD LTTQQILPYI DGFRHIQKIS AEADVELNLV RIAIQNLLYY GVVTLVSILQ YSNVYCPTPK VQDLVDDKSL QEACLSYVTK QGHKRASLRD VFQLYCSLSP GTTVRDLIGR HPQQLQHVDE RKLIQFGLMK NLIRRLQKYP VRVTREEQSH PARLYTGCHS YDEICCKTGM SYHELDERLE NDPNIIICWK
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.; Suppresses Src-dependent tyrosine phosphorylation and activation of PDPK1 and its downstream signaling. Down-regulates PDPK1 kinase activity by interfering with tyrosine phosphorylation at 'Tyr-9', 'Tyr-373' and 'Tyr-376' residues. May act as a tumor suppressor. Suppresses cell growth and enhances sensitivity to various anticancer drugs.
Gene References into Functions
  1. NPRL2 overexpression enhances sensitivity to CPT-11 treatment in colon cancer cells. PMID: 29519997
  2. This study demonstrated that NPRL2 mutation in familial focal epilepsies and focal cortical dysplasia. PMID: 27173016
  3. Data confirmed NPRL2 was downregulated in gliomas. More importantly, NPRL2 was able to inhibit cell proliferation in vitro and repress tumorigenicity in vivo, suggesting its role as a tumor suppressor. PMID: 26455908
  4. The positive regulation of mTORC1 activity by NPRL2 is mediated through NPRL2 interaction with Raptor. PMID: 26582740
  5. NPRL2 mutations are significant cause of focal epilepsy. PMID: 26505888
  6. NPRL2 acts as a functional tumor suppressor in colorectal cancer cell lines. PMID: 26044952
  7. NPRL2 enhances sensitivity to oxaliplatin in colon cancer cells. PMID: 25777765
  8. Decreased expression of NPRL2 is associated with renal cancer. PMID: 24789683
  9. NPRL2 mRNA blood levels could be a potentially useful marker for the detection of early stage adenomas and colorectal cancers. PMID: 24521741
  10. Results provide a set of genetic and biologic proofs that TUSC4 functions as a bona fide tumor suppressor by regulating the protein stability and function of BRCA1 in breast cancer. PMID: 25480944
  11. NPRL2 expression is negatively related with the survival of osteosarcoma patients, indicating its value as a prognosis factor of osteosarcoma. PMID: 23321467
  12. Lower NPRL2 expression was observed significantly more frequently in poorly differentiated tumor samples than in highly or moderately differentiated colon tumors. PMID: 23079973
  13. Data indicate that Npr2, a homolog of human NPRL2, is a phosphorylation-dependent target of the SCF(Grr1) E3 ubiquitin ligase that plays a role in cell growth on some nitrogen sources. PMID: 20154027
  14. NPRL2 is a multiple tumor suppressor gene. PMID: 15374952
  15. Expression of NPRL2 was significantly and reciprocally correlated to cisplatin sensitivity. PMID: 17018626
  16. TUSC4/NPRL2, a novel PDK1-interacting protein, plays a role in regulating the Src/PDK1 signaling pathway and cell sensitivity to multiple cancer chemotherapeutic drugs. PMID: 18616680
  17. Our results suggest that NPRL2 mRNA expression has prognostic significance for the survival of patients with HCC. PMID: 19274676

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Involvement in disease Epilepsy, familial focal, with variable foci 2 (FFEVF2)
Subcellular Location Lysosome membrane.
Protein Families NPR2 family
Tissue Specificity Most abundant in skeletal muscle, followed by brain, liver and pancreas, with lower amounts in lung, kidney, placenta and heart. Expressed in the frontal lobe cortex as well as in the temporal, parietal, and occipital lobes. Expressed in most lung cancer
Database Links

HGNC: 24969

OMIM: 607072

KEGG: hsa:10641

STRING: 9606.ENSP00000232501

UniGene: Hs.437083

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