Recombinant Human Nuclear ubiquitous casein and cyclin-dependent kinases substrate (NUCKS1)

Code CSB-YP867127HU
MSDS
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Source Yeast
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Code CSB-EP867127HU
MSDS
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Source E.coli
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Code CSB-EP867127HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP867127HU
MSDS
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Source Baculovirus
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Code CSB-MP867127HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NUCKS1
Uniprot No.
Alternative Names
FLJ21480; FLJ32016; FLJ38536; JC7; NUCKS 1; NUCKS; NUCKS_HUMAN; Nucks1; Nuclear casein kinase and cyclin dependent kinase substrate 1; Nuclear ubiquitous casein and cyclin dependent kinases substrate; Nuclear ubiquitous casein and cyclin-dependent kinases substrate; Nuclear ubiquitous casein kinase and cyclin dependent kinase substrate; P1; Potential LAG1 interactor
Species
Homo sapiens (Human)
Expression Region
1-243
Target Protein Sequence
MSRPVRNRKV VDYSQFQESD DADEDYGRDS GPPTKKIRSS PREAKNKRRS GKNSQEDSED SEDKDVKTKK DDSHSAEDSE DEKEDHKNVR QQRQAASKAA SKQREMLMED VGSEEEQEEE DEAPFQEKDS GSDEDFLMED DDDSDYGSSK KKNKKMVKKS KPERKEKKMP KPRLKATVTP SPVKGKGKVG RPTASKASKE KTPSPKEEDE EPESPPEKKT STSPPPEKSG DEGSEDEAPS GED
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Chromatin-associated protein involved in DNA repair by promoting homologous recombination (HR). Binds double-stranded DNA (dsDNA) and secondary DNA structures, such as D-loop structures, but with less affinity than RAD51AP1.
Gene References into Functions
  1. our results suggested that there was a significant association of a minor allele (G) in rs823114 with reduced risk of Parkinson's disease development (P = 0.017, OR = 0.768, 95%CI = 0.618 - 0.955). We conclude that NUCKS1 rs823114 indicates a decreased risk of susceptibility to Parkinson's disease and shows a male genetic distribution bias in the Han Chinese population. PMID: 29607885
  2. High NUCKS1 expression is associated with drug resistance in lung cancer. PMID: 26989074
  3. NUCKS overexpression had prognostic potential for overall survival and disease-free survival in advanced ovarian cancers and only for disease-free survival in early ovarian cancers . Our data suggest that NUCKS overexpression may contribute to progression and poor prognosis in ovarian cancer especially in advanced ovarian cancer PMID: 28877654
  4. NUCKS1 was identified as a potential oncogene at chromosomal 1q32.1 in patients with HCC, and it might be a valuable immunodiagnostic marker for HCC PMID: 26968889
  5. The results show that NUCKS1 is a chromatin-associated protein with a role in the DNA damage response and in homologous recombination, a DNA repair pathway critical for tumor suppression. PMID: 26323318
  6. NUCKS is a physiological regulator of energy homeostasis and glucose metabolism that works by regulating chromatin accessibility and RNA polymerase II recruitment to the promoters of IR and other insulin pathway modulators. PMID: 24931609
  7. NUCKS1 expression did not increase Tat nuclear localization. PMID: 25116364
  8. NUCKS1 overexpression may be associated with tumour progression and recurrence in cervical squamous cell carcinoma PMID: 24819170
  9. NUCKS-1 overexpression is associated with obesity and with breast cancer. PMID: 24568505
  10. We would like to emphasize that NUCKS abundance in malignant skin tumors is higher than that of the well-known proliferation marker Ki67, thus allowing more precise assessment of tumor proliferation potential. PMID: 25119009
  11. Authors suggest that NUCKS is involved in cellular responses following PDT. PMID: 24284105
  12. The expression of Nucks in both proliferating and quiescent cells and its correlation with Bax levels and apoptosis strongly suggest that Nucks plays complex roles in cell homeostasis. PMID: 24140890
  13. Through a combination of gene expression and copy number analysis, NUCKS1 was identified as a candidate gene involved in the distant metastasis of colorectal cancer. PMID: 23065711
  14. The results show overexpression of the NUCKS protein and RNA in a number of non malignant breast lesions and cancerous tissues. In particular, the NUCKS overexpression in ADH and DCIS indicates a significant role of this protein in neoplastic progression. PMID: 19664271
  15. Overexpression of NUCKS can play significant role in breast cancer biology. PMID: 20069058
  16. Two putative nuclear localization signals have been identified and characterized in NUCKS. PMID: 17604136
  17. NUCKS appears to have the highest ratio of modified to unmodified residues of any protein so far described. PMID: 18491381

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Subcellular Location
Nucleus. Chromosome.
Tissue Specificity
Widely expressed, with highest levels in thyroid gland, prostate and uterus and in fetal liver, thymus and lung.
Database Links

HGNC: 29923

OMIM: 611912

KEGG: hsa:64710

STRING: 9606.ENSP00000356110

UniGene: Hs.213061

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